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新型抗高血壓藥物-依普沙坦
...斷劑,是由史克必成公司開發,其商品名爲Teveten。別名SKF108566[1]。1藥物動力學1.1吸收依普沙坦口服後吸收迅速,富含脂質食物,減慢吸收,但不影響吸收率。生物利用度約13%,這是口服吸收不完全引起的。正常青年人血藥濃...
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DesensitizationoftheDopamineD1andD2ReceptorHetero-OligomerMediatedCalciumSignalbyAgonistOccupancyofEitherReceptor
...oro-7,8-dihydroxy-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepinehydrobromide(SKF-81297)ortheD2-selectiveagonistquinpirole.DesensitizationofthecalciumsignalmediatedbyD1-D2receptorhetero-oligomerswasinitiatedbyagonistoccupancyofeitherreceptorsubtypeeventhoughthesignalwasgeneratedonlybyoccupancyofbothrec...
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DifferentialActivationofAdenylateCyclaseandReceptorInternalizationbyNovelDopamineD1ReceptorAgonists
...andbenzazepines[7,8-dihydroxy-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine(SKF38393),(±)-7,8-dihydroxy-3-allyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine(SKF77434),6-chloro-7,8-dihydroxy-3-allyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine(SKF82958),3-methyl-6-chloro-7,8-hydroxy-1-[3-methylphenyl...
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ReducedrenaldopamineD1receptorfunctioninstreptozotocin-induceddiabeticrats
...tdecreaseininsulinlevelscomparedwithcontrolrats.IntravenousadministrationofSKF-38393,aD1receptoragonist,causedathreefoldincreaseinsodiumexcretionincontrolrats.However,SKF-38393failedtoproducenatriuresisindiabeticrats.SKF-38393causedaconcentration-dependentinhibitionofNa-K-ATPaseactivityinrenalproxim...
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RenaldopamineD1receptordysfunctionisacquiredandnotinheritedinobeseZuckerrats
...pithelialcells(PTECs)fromSprague-Dawley(SD)ratstoinsulinbluntedtheabilityofSKF-38393,aD1-receptoragonist,toinhibitNKAactivity.AdecreaseinD1receptornumberanddefectivereceptor-GproteincouplingledtothefailureofSFK-38393toinhibitNKAactivityininsulin-exposedPTECs(2).Ithasbeenreportedthatrenalproximaltubu...
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Voltage-DependentBlockofN-Methyl-D-aspartateReceptorsbyDopamineD1ReceptorLigands
...alf-block,andkbisthevoltagedependenceoftheblock.Fig.1.EffectsoftheD1agonistSKF81297onNMDAreceptor(NR1A/NR2A)mediatedcurrents.A,controlresponseto100µMglutamate.B,30µMSKF81297(blackbar)significantlyinhibitedtheNMDAreceptormediatedcurrent.C,noinhibitionwasdetectedwhenSKF81297wasonlyappliedb...
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Agonist-InducedCellSurfaceTraffickingofanIntracellularlySequesteredD1DopamineReceptorHomo-Oligomer
...hendopaminewasused.Cellsweretreatedwithoneofthefollowingagonists(pergolide,SKF81297,SKF38393,apomorphine,ordopamine),antagonists(SCH23390),orinverseagonists[(+)-butaclamol,flupenthixol,orfluphenazine].RadioligandBinding.Near-maximalsaturationreceptorbindingwasdeterminedwith15to25µgofmembranepr...
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RoleofoxidativestressindefectiverenaldopamineD1receptor-GproteincouplingandfunctioninoldFischer344rats
...ultinginrestorationofD1receptor-GproteincouplingandthenatriureticresponsetoSKF-38393.Thustempol,byloweringoxidativestress,normalizestheage-relateddeclineindopaminereceptorfunction.【關鍵詞】GproteinreceptorkinasesantioxidanttempolSKFAGINGISASSOCIATEDWITHANincreaseinoxidativestressandischaracter...
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30例嚴重少精症、無精子症患者的遺傳學分析
...目的探討嚴重少精症和無精子症與遺傳因素的關係。方法260例染色體G帶分析;116例Y染色體AZF區18個微缺失位點進行多重PCR基因擴增檢測分析。結果30例嚴重少精症和無精子症患者發現遺傳異常,其中26例染色體異常,4例AZF基因有...
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BooksReceived
...ainAdolescents—AFamily-BasedApproach,DanielleGrangeandJamesLock,2007,260pages,hardcover,$35.00.TheGuilfordPress,72SpringStreet,NewYork,NY10012.Internet:www.guilford.com.TheHealthImpactofSmokingandObesityandWhattoDoAboutIt,HansKrueger,DanWilliams,BarbaraKaminsky,andDavidMcLean,2007,374pages,sof...
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InvolvementofaDysfunctionalDopamine-D1/N-Methyl-D-aspartate-NR1andCa2+/Calmodulin-DependentProteinKinaseIIPathwayintheImpairmentofLatentLearni
...endentproteinkinaseII(CaMKII)inhibitor],KN92(aninactiveCaMKIIinhibitor),andSKF81297(adopamine-D1receptoragonist)werepurchasedfromSigma-Aldrich(St.Louis,MO).PCP,glycine,andSKF81297weredissolvedinsalinesolution.KN92andKN93weredissolvedina0.01%dimethylsulfoxide-containingsalinesolution.PCPwasadminister...
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藥物篩選技術爲遺傳疾病藥物研發提供新途徑
...KAP的表達,而IKBKAP是Riley-Day綜合症中的致病基因。其中,SKF-86466能夠通過調節細胞內PKA依賴的CREB磷酸化和cAMP水平來誘導IKBKAP轉錄,是在細胞水平上中止或逆轉疾病進程的潛在分子。Lee介紹道,這類疾病開展臨牀試驗難度較大,...
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WorkloadDuetoAspergillusfumigatusandSignificanceoftheOrganismintheMicrobiologyLaboratoryofaGeneralHospital
...edlaboratoryandclinicalparameterswasapplied.Wefound404A.fumigatusisolatesin260patients(1/1,000microbiologylaboratorysamples;2.1patients/10,000admissions).Atotalof90isolates(22.3%)werefrompatientswithIA.Ofthe260patients,31(12%)hadinvasivedisease(IA),andtheremaining229had“nonsignificant“disease.As...
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山東省大學生肥胖與體質健康的關係
...褶、腹皮褶等。用迴歸方程:男身體密度=1.0977-0.00146(∑2SkF1),女:身體密度=1.0931-0.00160(∑2SkF1),其中,∑2SkF1:肱三頭肌與肩胛下角處皮褶厚度之和[2]。體脂轉化公式(Brozek公式)爲:體脂百分比=[(4.570/Db-4.142)]×100%,Db爲...
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TempolreducesoxidativestressandrestoresrenaldopamineD1-likereceptor-Gproteincouplingandfunctioninhyperglycemicrats
...關鍵詞】GproteinreceptorkinasesproteinkinaseCantioxidantstreptozotocinSKFRENALDOPAMINEPLAYSANIMPORTANTroleinmaintainingsodiumhomeostasis,particularlyduringincreasesinsodiumintake.Intherenalproximaltubule,dopamineinhibitstheactivityofsodiumtransporterssuchasthesodiumhydrogenexchanger3andNa-K-ATPa...
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ReceptorRegulationofGeneExpressionofAxonGuidanceMolecules:ImplicationsforAdaptation
...oro-7,8-dihydroxy-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepinehydrobromide(SKF81297)alteredEphA5,EphrinA1,EphrinA5,andneuropilin1.cAMPandothersignaltransductionpathwaysmayregulategeneexpressionofaxonguidancemolecules,potentiallylinkingmonoaminereceptoractivationtosignaltransductioncascades,transcrip...
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ActivationofdopamineD1-likereceptorsinducesacuteinternalizationoftherenalNa+/phosphatecotransporterNaPi-IIainmousekidneyandOKcells
...atedkidneysliceswiththeselectiveD1-likereceptoragonistsfenoldopam(10μM)andSKF-38393(10μM)for1hinducedNaPi-IIainternalizationandreducedexpressionofNaPi-IIainthebrushbordermembrane(BBM).TheD2-likeselectiveagonistquinpirole(1μM)hadnoeffect.TheD1andD2agonistsdidnotaffecttherenalNa+/sulfatecotransport...
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用核酸序列分析鑑別甲肝病毒疫苗株與野毒株
...驗動物中心,公母各一,編號103,106,猴齡2~3歲,體重260~350克。感染前經檢測血清抗-HAV陰性,ALT、AST、ICD等肝臟酶活性和肝組織病理檢查均正常。該品系狨猴對甲肝病毒感染的敏感性高,是研究甲肝病毒可靠動物模型〔4〕...
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生物檢材中利血平的高效液相色譜/質譜和高效液相色譜的檢測
...定所,利血平片(石家莊市太行醫藥有限公司提供);內標:SKF525A(中國標準技術開發公司);10%鹽酸溶液;乙腈爲色譜純,其他試劑均爲分析純,水爲重蒸水。 1.2儀器與色譜條件 島津LC210ATvp高效液相色譜儀,SPD210AVvp紫外可見檢...
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ComparisonoftotalbodypotassiumwithothertechniquesformeasuringleanbodymassinmenandwomenwithAIDSwasting
...(DXA),bioelectricalimpedanceanalysis(BIA),andskinfold-thicknessmeasurement(SKF)in132patients(63men,69women)withAIDSwasting(weight90%ofidealbodyweight,orweightloss10%oforiginal,orboth).Noneofthesubjectsexhibitedclinicallipodystrophy.ComparisonsweremadebyusingdifferentBIAequations.Results:Leanbodymass...
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將幹細胞應用於藥物開發
...人員很快就確定了8個化合物並進行進一步的測試,其中SKF-86466最終證明在細胞水平上能停止或逆轉疾病的進程。實驗結果的影響不只限於Riley-Day綜合徵,有許多罕見的遺傳性疾病是通過昂貴的藥物開發模式來解決的。作者:
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p38MAPkinaseinhibitionamelioratescisplatinnephrotoxicityinmice
...reaseininfiltratingleukocytes.However,animalstreatedwiththep38MAPKinhibitorSKF-86002alongwithcisplatinshowedlessrenaldysfunction(BUN:55±14mg/dl,creatinine:0.3±0.02mg/dl,P<0.05),lessseverehistologicaldamage,andfewerleukocytescomparedwithcisplatin+vehicle-treatedanimals.SerumlevelsofTNF-,sTNFRI,ands...
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沙芬僞麻咀嚼片
...加入十二烷基硫酸鈉2.0g和磷酸1.0ml爲流動相,檢測波長爲260nm,理論板數按鹽酸僞麻黃鹼計算,應不低於2000;鹽酸僞麻黃鹼和氫溴酸右美沙芬的分離度均應符合要求。測定法精密稱取氫溴酸右美沙芬對照品15mg及經105℃乾燥至恆...
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AnalyticalVerificationofaPCRAssayforIdentificationofBordetellaavium
...aviumchromosomalDNA,rangingfrom25pgto1,300pg,wasestimatedtobebetween25pgand260pg(K.B.Register,unpublisheddata).TheeffectsofalteringtheconcentrationofDMSO,MgCl2,orprimersonproductyieldofPCRscontainingtemplateamountswithinthisrangewerenextcompared.Optimalconcentrationswereidentifiedas5%DMSO,0.5μMprim...
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HPLC法測定苦玄蔘中苦玄蔘苷IA的含量
【摘要】目的建立苦玄蔘藥材PicriafelterraeLour.的含量測定方法。方法RP-HPLC法,採用C18ODS2柱(5μm,4.6×250mm),乙腈-0.3%磷酸(34∶66)爲流動相,流速爲1ml/min,檢測波長爲264nm。結果苦玄蔘苷IA進樣量在0.42~2.10μg的範圍內呈良好的線性...
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辛伐他汀片
...液,照分光光度法(中國藥典1995年版二部附錄IVA),於260~210nm的波長範圍內測定吸收度,在247、238與231nm的波長處有最大吸收。(2)在含量測定項下記錄的色譜圖中,供試品峯的保留時間應與對照品峯的保留時間一致。 檢...
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第二節 MHC抗原的結構及檢測
...方塊表示尚未確定的多肽摺疊。 2.I區相關抗關 (1)Ia抗原的概念:Klein、Shrefler(1974、1975年)將小鼠I區基因所編碼的抗原稱爲Ia抗原(Iregionassociatedantigen),也有人將I區中的Ir基因的產物稱爲Ia抗原。目前一般把人類Ⅱ類抗原,尤...
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胰島β細胞自身抗體IA-2Ab的ELISA方法建立——附110例正常人測定值
....4P.294-2969(天津)爲了以本實驗室研製的重組人自身抗原IA-2作爲抗原,建立血清中抗IA-2的自身抗體的ELISA測定方法,並對正常人羣進行檢測。研究者在間接ELISA法建立過程中對封閉劑、血清稀釋度及二抗稀釋度及其他測試條件進...
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糖尿病相關胰島自身抗體檢測的臨牀意義
...(ICA)、穀氨酸脫羥酶抗體(GADA)、胰島素自身抗體(IAA)、以及蛋白酪氨酸磷酸酶抗體(IA-2A)等四種抗體的臨牀意義較大。本文複習了近年文獻報道的一些結果。 1檢測方法的完善與標準化 ICA檢測是臨牀上應用較早...
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CT和PET:Ia期肺癌診斷分期偏低
...eting.2008May10-14醫學空間(MEDcyber.com)7月3日消息-臨牀分期Ia期非小細胞肺癌目前傾向於縮小切除範圍。這一治療策略依賴於臨牀分期的準確,這依賴於影像學檢查技術。紐約的Brendon等研究考察了CT和PET在患者臨牀分期的準確性,...