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點突變p53肽誘導肽特異性CTL的研究
...ontNEN,美國)。 4.肽合成:P132F肽(LNKLFFQL)和仙台病毒肽SEV-9(FAPGNYPAL,作爲對照肽)的合成見參考文獻〔2〕。 5.肽免疫、CTL誘導及其殺傷活性測定:肽與佐劑(IFA或RAS)充分混勻,給C57BL/6小鼠免疫(尾根部皮下注射,免疫劑量爲100...
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北大、武大《PNAS》文章介紹MDA5的一種生理抑制劑
...或TLR3介導的IFN-Β感應。DAK過量表達還會抑制細胞質dsRNA和SeV誘導的IFN-β啓動子的活化;而RNAi沉默內源性DAK後,IFN-β啓動子活化,dsRNA或者Sev激發的INF-β的活化水平上升。另外,DAK的過量表達會抑制MDA5但不會抑制RIG-I介導的抗病毒...
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Alcoholintakeinrelationtobrainmagneticresonanceimagingfindingsinolderpersonswithoutdementia
...fmanandMoniqueMBBreteler1FromtheDepartmentsofEpidemiologyBiostatistics(TdH,SEV,EjvD,NDP,CMvD,AH,andMMMB)andofNeurology(TdH,SEV,EjvD,NDP,andPJK),ErasmusMedicalCenter,Rotterdam,Netherlands.2SupportedbytheNetherlandsOrganizationforScientificResearch(NWOgrant904-61-096),theNetherlandsHeartFoundation(gra...
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AIRE研究
...tients,aged18years,withevidenceofheartfailure3–10daysafterMI;patientswithsevereheartfailure(usuallyNYHAclassIV)orongoingischemiaexcludedFollowupandprimaryendpointAverage15monthsfollowup.Primaryendpointall-causemortalityTreatmentPlaceboorramiprilinitiatedat2.5mgtwicedaily;increasedto5mgtwicedailyaf...
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隔蛋白7逆轉裸鼠移植膠質瘤的惡性表型
...TJ905細胞的裸鼠皮下移植瘤模型,將荷瘤鼠分爲治療組(SEVIT/pcDNA3)、空載體組(pcDNA3)和對照組(PBS),觀察各組腫瘤的大小及生長速度;採用免疫組化法檢測各組腫瘤組織標本中SEPT7、增殖細胞核抗原(PCNA)、膠質纖維酸...
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武漢病毒所天然免疫通路調控研究取得進展
...P抑制MITA介導的I型干擾素信號通路,即使存在仙台病毒(SeV)和細菌第二信號分子(c-di-GMP)的刺激過表達MRP仍可以劑量依賴的抑制MITA介導的天然免疫通路。後期的研究表明MRP的抑制機制主要是通過和MITA作用從而影響MITA和TBK1的...
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NonendothelialMesenchymalCell-DerivedMCP-1IsRequiredforFGF-2-MediatedTherapeuticNeovascularization
...ctor)andarteriogenic(MCP-1)signalsviaindependentsignaltransductionpathways.SeverehindlimbischemiastimulatedMCP-1expressionthatwasstronglyenhancedbyFGF-2genetransfer,andablockadeofMCP-1activityviaadominantnegativemutantaswellasadeficiencyofitsfunctionalreceptorCCR2resultedinthediminishedrecoveryofblo...
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Cognitiveperformanceamongtheelderlyanddietaryfishintake:theHordalandHealthStudy
...slo,Norway(EN,CAD,HRandKS);theDepartmentofPublicHealthandPrimaryHealthCare(SEV,HAN,andGST)andtheInstituteofMedicine(ON),UniversityofBergen,Bergen,Norway;andtheDepartmentofGeriatricMedicine,NorwegianCentreforDementiaResearch,UllevålUniversityHospital,Oslo,Norway(KE)Seecorrespondingeditorialonpa...
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新病毒誘導I型干擾素負性調控機制
...頭蛋白VISA的相互作用,從而抑制由仙台病毒(SendaiVirus,SeV)引起的IRF3入核以及I型干擾素的產生。在HCV慢性感染的病人外周血細胞中,發現TBK1s的表達高低與HCV慢性感染相關聯。本研究成果對進一步豐富I型干擾素產生的調控網絡...
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昆明動物所揭示新型HIV病毒限制因子TRIMCyp的起源和功能
...成的。有趣的是,樹鼩TRIMCyp而非TRIM5-4能負調控仙台病毒(SeV)介導的抗病毒天然免疫應答,下調NF-κB和IFN-β的表達,提示樹鼩TRIMCyp喪失了HIV病毒限制功能而產生了新的免疫調控功能。該研究不僅拓展了病毒限制因子TRIM5/TRIMCyp的功...
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Incidenceofkeratitisofvaryingseverityamongcontactlenswearers
...Acceptedforpublication29August2004ABSTRACTAim:Todeterminetheincidenceofnon-severekeratitis(NSK)andseverekeratitis(SK)amongwearersofcurrentgenerationcontactlenses.Methods:A12month,prospective,hospitalbasedepidemiologicalstudywasconductedbyexaminingallcontactlenswearerspresentingwithacornealinfiltrate...
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β1整合素表達下調對人結腸癌細胞增殖和化療敏感性影響
...ta;兩個亞基組成的異二聚體,β亞基的胞漿區含有Asp-X-Sev-X-Sev序列,參與細胞骨架蛋白的相互作用和胞內信號傳導。整合素將胞外的ECM分子與細胞內的骨架蛋白連接起來形成焦點黏附物。焦點黏附物是整合素內外雙向信號傳導...
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PlasmavitaminB-6formsandtheirrelationtotranssulfurationmetabolitesinalarge,population-basedstudy
...ysteineandRelatedVitamins,UniversityofBergen,Bergen,Norway(ØM,SH,JS,SEV,andPMU);theHormoneLaboratory,HaukelandUniversityHospital,Bergen,Norway(SH);theSectionforPharmacology,InstituteofMedicine,UniversityofBergen,andHaukelandUniversityHospital,Bergen,Norway(SH);andtheDepartmentofBiomedicalScie...
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MolecularPhenotypesofAtherosclerosis
...ncedlesionisdesirable.Thepresenceatautopsyofatheroscleroticlesionsofvaryingseverityandclassifications1withinthesameindividualreflectsthecomplexityofthischronicinflammatoryprocess.Furthermore,certainarteriallocationsarepredictiveforlesionformationbeforeanydetectablepathologicalchangeisapparent,wherea...
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Patternsandpredictorsoffolicacidsupplementuseamongpregnantwomen:theNorwegianMotherandChildCohortStudy
...ologyandMedicalStatistics,DepartmentofPublicHealthandPrimaryHealthCare(RMN,SEV,andHKG),andtheLOCUSforHomocysteineandRelatedVitaminsandtheSectionforPharmacology,InstituteofMedicine(PMU),UniversityofBergen,Bergen,Norway;theMedicalBirthRegistry,NorwegianInstituteofPublicHealth,Bergen,Norway(SEV);andthe...
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RelationsofglutamatecarboxypeptidaseII(GCPII)polymorphismstofolateandhomocysteineconcentrationsandtoscoresofcognition,anxiety,anddepression
...ord,UnitedKingdom(HRandADS);andtheUniversityofBergen,Bergen,Norway(OKN,PMU,SEV,andGST)2HRiscurrentlyattheDepartmentofNutrition,InstituteofBasicMedicalSciences,UniversityofOslo,Oslo,Norway.3SupportedbyresearchgrantsDK56085andDK35747fromtheUSNationalInstitutesofHealthtoCHHandbyagrantfromtheNormanColli...
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Dietaryfatandplasmatotalhomocysteineconcentrationsin2adultagegroups:theHordalandHomocysteineStudy
...ay(PB,HR,EN,CAD,andGU);theDepartmentofPublicHealthandPrimaryHealthCare(SVK,SEV,andGST)andtheSectionforPharmacology,InstituteofMedicine(HRandPMU),UniversityofBergen,Norway;theOxfordCentreforGeneFunction,DepartmentofPhysiology,AnatomyGenetics,UniversityofOxford,UnitedKingdom(HR);andtheDepartmentofPrev...
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DetectionofHumanInfluenzaAVirusesbyLoop-MediatedIsothermalAmplification
....Earlyidentificationofthevirusisthereforecriticalforthecontrolofthedisease.Severalmoleculartechniqueshavebeendevelopedasrapidtestsforclinicaldiagnosisofinfluenzavirus(1,10).Therecentinventionofloop-mediatedisothermalamplification(LAMP)providesanewalternativeformoleculardiagnosis(8).Oneofthemajoradva...
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Bodymassindexandfatmassaretheprimarycorrelatesofinsulinresistanceinnondiabeticstage3–4chronickidneydiseasepatients
...donESRDpatients,butanincreasingrecognitionofthehighprevalenceofmoderate-to-severeCKDhasredirectedtheattentiontothispatientpopulationtoidentifyriskfactorsassociatedwithhospitalization,death,andprogressiontoESRD.Indeed,studieshaveshownthatthereisagreaterriskofatheroscleroticeventsandahigherriskofdeath...
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Changesinlifestyleandplasmatotalhomocysteine:theHordalandHomocysteineStudy
...dPerMUeland1FromtheDepartmentsofPublicHealthandPrimaryHealthCare(EN,GST,andSEV)andPharmacology(HRandPMU),theInstituteofMedicine(ON),andtheLocusforHomocysteineandRelatedVitamins(EN,GST,SEV,ON,andPMU),theUniversityofBergen,Norway;theDepartmentofPharmacology,UniversityofOxford,UnitedKingdom(HR);andtheM...
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ThemetabolicsyndromeinadultsprenatallyexposedtotheDutchfamine
...49;prenatalfaminebirthweightINTRODUCTIONThemetabolicsyndromeisknownbyseveralnames:syndromeX,thedeadlyquartet,andtheinsulinresistancesyndrome(1-3).Itisaconstellationofinterrelatedmetabolicriskfactorsthatpredisposesapersontodeveloptype2diabetesandcardiovasculardisease(4,5).Clinicalmanifestations...
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ApplicationofaMolecularPanelToDemonstrateEnterotropicVirusSheddingbyHealthyandHumanImmunodeficiencyVirus-InfectedPatients
...Department,VastoHospital,Vasto,ItalyABSTRACTWeusedamolecularpanel,targetingsevenentericviruses,toexploretheadvantageofusingmolecularmethodstoestablishtheetiologyofentericdiseasesandtoevaluatetheprevalenceofentericvirusesinasymptomatichumanimmunodeficiencyvirus-infectedpatients.Thisapproachfavorsrapi...
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EssentialroleofIPS-1ininnateimmuneresponsesagainstRNAviruses
...hecriticalroleofIPS-1inantiviralresponsesinvivo.IPS-1–deficientmiceshowedseveredefectsinbothRIG-I–andMda5-mediatedinductionoftypeIinterferonandinflammatorycytokinesandweresusceptibletoRNAvirusinfection.RNAvirus–inducedinterferonregulatoryfactor-3andnuclearfactorBactivationwasalsoimpairedinIPS-...
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DevelopmentofaTriplexReal-TimePCRAssayforDetectionofPanton-ValentineLeukocidinToxinGenesinClinicalIsolatesofMethicillin-ResistantStaphyloco
...n2002(8).Recently,MRSAstrainsproducingPVLhavebeenassociatedwithanincreaseinsevereskininfectionsandanewsyndromeofcommunity-associatednecrotizingpneumoniainchildren(19).ThePVLgenesarecarriedonthephageSLT(15),buttodate,PVLhasnotbeenidentifiedwithepidemicorendemichospital-acquiredMRSAstrains(12).Withthe...
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腦脊液鼻漏致複發性化膿性腦膜炎的外科診治探討
...m1982to2004werereviewed.Allofcasesunderwentsurgicaltreatment.ResultsThirty-sev-enoperationsweredoneon25cases.ThetotalsuccessrateofclosureofCSFrhinorrheawas92%.Elevencasesweretreatedbyneurosurgeon.Ofthe11cases,9receivedrepairsurgeryinextracranialapproachesthroughsphenoidsinus,and2casesinextradura...
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Plasmaconcentrationoffolateasabiomarkerfortheintakeoffruitandvegetables:theHordalandHomocysteineStudy
...ofOslo(AB,EBL,CAD,andLFA);theDepartmentsofPublicHealthandPrimaryHealthCare(SEVandGST)andPharmacology(HRandPMU),UniversityofBergen,Bergen,Norway;andtheDepartmentofPharmacology,UniversityofOxford,Oxford,UnitedKingdom(HR)2SupportedbytheNorwegianInstituteofPublicHealth,TheCommunityHealthServiceofHordala...
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InhaledCorticosteroidsandtheBeneficialEffectofDeepInspirationinAsthma
...orticosteroidsonthesephenomena.Twogroupsofsubjectswithasthma,9withmoderate/severehyperresponsivenesstomethacholine,and12withmild/borderlinehyperresponsivenesstomethacholine,receivedinhaledfluticasone(880edaily)for12weeks.SerialbronchoprovocationswereperformedatWeeks0,6,and12.Theimpactofdeepinspirati...
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保留喉功能下嚥癌手術切除與修復
...tsThe3-yearsurvivalratewas73.9%(17/23)and5-yearsurvivalratewas47.8%(11/23).Severecomplicationoccurredin8of23(34.8%).Completerestorationoflaryngealfunctionwasachievedin20cases(87%)anddeglutitionrestoredinallpatients(100%).Themeandurationoforalintakewas11.8days(6~33days)andmeanhospitalizationwas32da...
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AssociationBetweenCarotidPlaqueCharacteristicsandSubsequentIschemicCerebrovascularEvents
...ompositionsuchasthesizeofthelipidcoreandpresenceofintraplaquehemorrhage.1,2Severalprospectivestudiesusingultrasoundhaveshownthatecholucentcarotidplaquesareassociatedwithincreasedriskforcerebrovascularevents.3–6Echolucentplaquesareknowntohaveahighercontentoflipidandhemorrhagethandoechogenicplaques,...
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Paediatriccataractblindnessinthedevelopingworld:surgicaltechniquesandintraocularlensesinthenewmillennium
...edtoavoidnystagmusandalifetimeofpoorvisualacuity. Currently,approximatelysevenmillionadultcataractsurgeriesareperformedannuallyintheworld.15Precisedatarelatedtothetotalnumberofpaediatriccataractsurgeriesperformedannuallyarenotavailable.However,paediatriccataractblindnesspresentsanenormousproblemto...