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抗纖複方及TGF-β對膠原基因啓動子活性的影響①
...pCOL0.8重組體構建 含小鼠α2(Ⅰ)膠原-2kb~+54bp序列的質粒pAZ1009由美國NICCrombrugghe教授惠贈(參見圖1)[6],重新構建的重組體所用載體爲不含啓動子,但含有SV40增強子、氯黴素乙酰基轉移酶(CAT)報告基因的質粒pCAT-enhancer(promega...
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RNA干擾作用原理及其應用
...斷(siRNA)[5]。Dicer含有解旋酶活性以及dsRNA結合域和PAZ結構。在RNAi的效應階段,siRNA雙鏈結構解旋並形成有活性的蛋白/RNA複合物(RNA-inducedsilencingcomplexorRISC),在siRNA解雙鏈即RISC激活過程需一個ATP[5]。由RISC中siRNA反義鏈與...
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細胞因子對小鼠α2(I)型前膠原基因啓動子活性影響的研究①
...,將它與氯黴素乙酰基轉移酶(CAT)報告基因組成的重組體pAZ1009用脂質體法轉染至膠原產生細胞NIH/3T3細胞,討論IFNα、IFNγ、TNFα對小鼠α2(I)型前膠原基因上游啓動子活性的影響。結果:TNFα抑制小鼠α2(I)型前膠原基因啓動子活性...
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DoesResistancetoPyrazinamideAccuratelyIndicatethePresenceofMycobacteriumbovis
...A.Hahn,P.M.Small,C.L.Daley,M.A.Behr,A.R.Moss,J.M.Creasman,G.F.Schecter,E.A.Paz,andP.C.Hopewell.1999.AmolecularepidemiologicanalysisoftuberculosistrendsinSanFrancisco,1991-1997.Ann.Intern.Med.130:971-978.Kamerbeek,J.,L.Schouls,A.Kolk,M.vanAgterveld,D.vanSoolingen,S.Kuijper,A.Bunschoten,H.Molhuizen,R....
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SensitivitiesandSpecificitiesofSpoligotypingandMycobacterialInterspersedRepetitiveUnit-Variable-NumberTandemRepeatTypingMethodsforStudyingMole
...epidemiologicmethods.N.Engl.J.Med.330:1710-1716.Behr,M.A.,P.C.Hopewell,E.A.Paz,L.M.Kawamura,G.F.Schecter,andP.M.Small.1998.PredictivevalueofcontactinvestigationforidentifyingrecenttransmissionofMycobacteriumtuberculosis.Am.J.Respir.Crit.CareMed.158:465-469.Daley,C.L.,P.M.Small,G.F.Schecter,G.K.Schoo...
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RNA干涉過程中“小干涉RNA”的識別
...特定信使RNA進行降解的指導要素。現在,結構研究確定,PAZ(Argonaute蛋白家族特有的一個區域,該家族的蛋白與RNA沉寂機制有關)專門識別siRNA,方法是通過將siRNA的3個懸臂固定。作者:
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RNA誘導沉默複合體中的生物大分子及其裝配
...結構和功能的作用;Argonaute蛋白是RISC中的核心蛋白,有PAZ和PIWI兩個主要的結構域,前者爲siRNA的傳遞提供結合位點,後者是RISC中的酶切割活性中心;siRNA是RISC完成特異性切割作用的嚮導,在成熟的RISC中雖然只包含siRNA的一條鏈...
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牽牛花和RNA干擾
...basemadeupoftheamino-terminal,middle,andPIWIdomains.ThePiwiArgonauteZwille(PAZ)domainisheldabovethebasebyastalk-likeregion.ThePIWIdomain(namedfortheproteinpiwi)issimilartoribonucleaseH,withaconservedactivesiteaspartate-aspartate-glutamatemotif,stronglyimplicatingArgonauteasSlicer.Thearchitectureofth...
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研究發現抑制RNAi抗病毒先天免疫新機制
...制蛋白B2的研究發現,B2可以通過直接作用於果蠅Dicer-2的PAZ及RNaseIII結構域,從siRNA產生及RNA沉默複合體(RISC)組裝兩個層面抑制Dicer-2蛋白在細胞中的功能。這是第一次證明了宿主Dicer蛋白可以直接被病毒抑制,並詳細的闡釋了...
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噬菌體雞尾酒拯救葡萄
...,根據生理和遺傳性質將其分爲四種,Sano、Salvo、Prado和Paz,它們各不相同。”課題組成員TusharSuvraBhowmick博士說道。生長在溫室裏的葡萄接種了細菌和四種噬菌體雞尾酒製劑。實驗團隊既試驗了植物感染葉緣焦枯病菌後供應其噬...
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Anthropometricmeasuresinmiddleageafterexposuretofamineduringgestation:evidencefromtheDutchfamine
...ta,GA(HSK);theMailmanSchoolofPublicHealth,ColumbiaUniversity,NewYork,NY(AR,PAZ,andLHL);andTNOQualityofLife,Leiden,Netherlands(KvdP)2ThefindingsandconclusionsinthisreportarethoseoftheauthorsanddonotnecessarilyrepresenttheviewsoftheCentersforDiseaseControlandPrevention.3SupportedbygrantRO1HL067914(Pri...
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Long-TermMolecularAnalysisofTuberculosisStrainsinAlabama,aStateCharacterizedbyaLargelyIndigenous,Low-RiskPopulation
...iemer,P.M.Small,A.P.deLeon,R.Steinhart,G.F.Schecter,C.L.Daley,A.R.Moss,E.A.Paz,R.M.Jasmer,C.B.Agasino,andP.C.Hopewell.1998.DifferencesincontributingfactorstotuberculosisincidenceinU.S.-bornandforeign-bornpersons.Am.J.Respir.Crit.CareMed.158:1797-1803.Cronin,W.A.,J.E.Golub,M.J.Lathan,L.N.Mukasa,N.Hoo...
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RNA干擾技術及其在泌尿系腫瘤治療中的應用
...eotide)的siRNA(smallinterferenceRNA)雙鏈。②效應階段:Dicer通過PAZ結構域與含有PAZ結構域的Argonaute蛋白互相作用,核酸內外切酶、解旋酶與Argonaute蛋白相連,進而與siRNA一起形成具有多個亞單元的核糖核苷酸蛋白複合物(RNAinducingsilencingc...
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胸腔鏡術後發生肺漏氣的護理
...CardiovascSurg,1991,102:721.4CuschieriA,BerciG,Paz-PartlowM,etal.Laparoscopicbiliarysurgery,2nded.Oxford,Blackwellscientificpublications,1991,51-55.5孫靜.電視胸腔靜微創手術治療肺大泡護理體會.蚌埠醫學院學報,2003,28(2):648.作者單位:21406...
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ASCO2015帶給我的10大臨牀決策改變
...常昂貴。2.推薦Nivolumab用於難治性晚期非鱗非小細胞肺癌Paz-Ares等[2]在ASCO上報道,一項全球多中心III期臨牀研究發現,將接受鉑類爲基礎的雙藥方案進展的晚期非鱗非小細胞肺癌(NSCLC)患者隨機分爲Nivolumab組或多西他賽組,接...
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VascularEndothelialGrowthFactorIsInducedbytheInflammatoryCytokinesInterleukin-6andOncostatinMinHumanAdiposeTissueInVitroandinMurineAdip
...ndvisceralwhiteadiposetissueandinthehumanbrownadiposetissuederivedcell-linePAZ6.FurthermorewedeterminedpossibleeffectsofIL-6andOSMontheexpressionofVEGFinadiposetissueinmiceinvivo.MaterialsandMethodsPrimaryculturesofhumanvisceralandsubcutaneouspreadipocytes,adipocytes,andexvivocultureswerepreparedfro...
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MycobacteriummarinumInfectionsinFishandHumansinIsrael
...erizationofagenecodingfor16SribosomalRNA.NucleicAcidsRes.17:7843-7853.Even-Paz,Z.,H.Haas,T.Sacks,andE.Rosenmann.1976.Mycobacteriummarinumskininfectionsmimickingcutaneousleishmaniasis.Br.J.Dermatol.94:435-442.Falkinham,J.O.1996.Epidemiologyofinfectionbynontuberculousmycobacteria.Clin.Microbiol.Rev.9:...
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PresenceofDNAofHumanPapillomavirus16butNoOtherTypesinTumor-FreeTonsillarTissue
...lomavirustype16DNAincarcinomasofthepalatinetonsil.J.Clin.Pathol.43:918-921.Paz,I.B.,N.Cook,T.Odom-Maryon,Y.Xie,andS.P.Wilczynski.1997.Humanpapillomavirus(HPV)inheadandneckcancer.AnassociationofHPV16withsquamouscellcarcinomaofWaldeyer‘stonsillarring.Cancer79:595-604.Resnick,R.M.,M.T.Cornelissen,D.K...
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EndophthalmitisCausedbyFusariumproliferatum
...ology,andclinicalaspectsofFusariumspecies.Clin.Microbiol.Rev.7:479-504.Nir-Paz,R.,J.Strahilevitz,M.Shapiro,N.Keller,A.Goldschmied-Reouven,O.Yarden,C.Block,andI.Polacheck.2004.ClinicalandepidemiologicalaspectsofinfectionscausedbyFusariumspecies:acollaborativestudyfromIsrael.J.Clin.Microbiol.42:3456-3...
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AssociationsofPPARGC1AHaplotypesWithPlaqueScorebutNotWithIntima-MediaThicknessofCarotidArteriesinMiddle-AgedSubjects
...indingsiteandaffectsPGC-1expressioninINS1-EcellsbuthaslittleeffectinHepG2orPAZ6cells.6PreliminarystudiesinTHP-1orhumanumbilicalveinendothelialcellsrevealedminoreffectsofthisSNPontheexpressionofareportergeneunderbasalcultureconditions(datanotshown).However,phasedgenotypingin46allelesshowedcompletelin...
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EpidemiologicImportofTuberculosisCasesWhoseIsolatesHaveSimilarbutNotIdenticalIS6110RestrictionFragmentLengthPolymorphismPatterns
...berculosis.Theor.Popul.Biol.63:347-363.Small,P.M.,P.C.Hopewell,S.P.Singh,A.Paz,J.Parsonnet,D.C.Ruston,G.F.Schecter,C.L.Daley,andG.K.Schoolnik.1994.TheepidemiologyoftuberculosisinSanFrancisco.Apopulation-basedstudyusingconventionalandmolecularmethods.N.Engl.J.Med.330:1703-1709.Thierry,D.,M.D.Cave,K.D...
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MolecularFingerprintingofLeishmaniainfantumStrainsfollowinganOutbreakofVisceralLeishmaniasisinCentralIsrael
...orthernMinnesotabeaverponds.Proc.Natl.Acad.Sci.USA92:5001-5005.Ephros,M.,A.Paz,andC.L.Jaffe.1994.AsymptomaticvisceralleishmaniasisinIsrael.Trans.R.Soc.Trop.Med.Hyg.88:651-652.Gdalevich,M.,D.Mimouni,I.Ashkenazi,andJ.Shemer.2000.RabiesinIsrael:decadesofpreventionandahumancase.PublicHealth114:484-487.G...
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DendriticcellsstarinVancouver
...indendriticcells.Nature.425:397–402.Zuniga,E.I.,D.B.McGavern,J.L.Pruneda-Paz,C.Teng,andM.B.Oldstone.2004.Bonemarrowplasmacytoiddendriticcellscandifferentiateintomyeloiddendriticcellsuponvirusinfection.Nat.Immunol.5:1227–1234.Lindquist,R.L.,G.Shakhar,D.Dudziak,H.Wardemann,T.Eisenreich,M.L.Dustin,...
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Flow-MediatedDilatationIsImpairedbyaHigh–SaturatedFatDietbutNotbyaHigh-CarbohydrateDiet
...lCardiol.1999;33:805–812.FuentesF,Lopez-MirandaJ,SanchezE,SanchezF,PaezJ,Paz-RojasE,MarinC,GomezP,Jimenez-PereperezJ,OrdovasJM,Perez-JimenezF.Mediterraneanandlow-fatdietsimproveendothelialfunctioninhypercholesterolemicmen.AnnInternMed.2001;134:1115–1119.deRoosNM,BotsML,SiebelinkE,SchoutenE,Katan...
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DiabeticMicroangiopathyinIschemicLimbIsaDiseaseofDisturbanceofthePlatelet-DerivedGrowthFactor-BB/ProteinKinaseCAxisbutNotofImpairedExpre
...etes.2002;51:1556eC1564.Guzman-GardearzabalE,Leyva-BohorquezG,Salas-ColinS,Paz-JaneiroJL,Alvarado-RuizR,Garcia-SalazarR.Treatmentofchroniculcersinthelowerextremitieswithtopicalbecaplermingel.01%:amulticenteropen-labelstudy.AdvTher.2000;17:184eC189.RivardA,SilverM,ChenD,KearneyM,MagnerM,AnnexB,Peters...
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AssociationsoftheUCP2GeneLocusWithAsymptomaticCarotidAtherosclerosisinMiddle-AgedWomen
...expression,increasedtranscriptionofareportergeneinthehumanadipocytecelllinePAZ6,anddecreasedriskofobesityinmiddle-agedhumans.12Ina?-cellenvironment,thevariantallelewasmoreeffectivelyactivatedbythetranscriptionfactorPAX6andwasassociatedwithareduceddispositionindex(theproductoftheacuteinsulinresponset...
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ReducedmitochondrialdensityandincreasedIRS-1serinephosphorylationinmuscleofinsulin-resistantoffspringoftype2diabeticparents
...ate-1tyrosinephosphorylationrequiresserine612.Biochemistry.36::12939-12947.Paz,K.etal.1999..Phosphorylationofinsulinreceptorsubstrate-1(IRS-1)byproteinkinaseBpositivelyregulatesIRS-1function.J.Biol.Chem.274::28816-28822.Hirosumi,J.etal.2002..AcentralroleforJNKinobesityandinsulinresistance.Nature.420...
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MolecularTypingofMycobacteriumtuberculosisbyMycobacterialInterspersedRepetitiveUnit-Variable-NumberTandemRepeatAnalysis,aMoreAccurateMethodfo
...l.39:69-74.Geng,E.,B.Kreiswirth,C.Driver,J.Li,J.Burzynski,P.DellaLatta,A.LaPaz,andN.W.Schluger.2002.ChangesinthetransmissionoftuberculosisinNewYorkCityfrom1990to1999.N.Engl.J.Med.346:1453-1458.Hawkey,P.M.,E.G.Smith,J.T.Evans,P.Monk,G.Bryan,H.H.Mohammed,M.Bardhan,andR.N.Pugh.2003.Mycobacterialintersp...
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LongQTsyndrome:fromchannelstocardiacarrhythmias
...ogicalandtemperatureeffects.J.Biol.Chem.274::31123-31126.Ficker,E.,Obejero-Paz,C.A.,Zhao,S.,andBrown,A.M.2002..ThebindingsiteforchannelblockersthatrescuemisprocessedhumanlongQTsyndrometype2ether-a-gogo-relatedgene(HERG)mutations.J.Biol.Chem.277::4989-4998.Paulussen,A.etal.2002..Anovelmutation(T65P)i...
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MProteinGene(emmType)AnalysisofGroupABeta-HemolyticStreptococcifromEthiopiaRevealsUniquePatterns
...-Goor,J.Jaffe,I.Shetzigovsky,M.Ravins,Z.Korenman,R.Cohen-Poradosu,andR.Nir-Paz.2003.emmTypingofMnontypeableinvasivegroupAstreptococcalisolatesinIsrael.J.Clin.Microbiol.41:4655-4659.Pruksakorn,S.,N.Sittisombut,C.Phornphutkul,C.Pruksachatkunakorn,M.F.Good,andE.Brandt.2000.Epidemiologicalanalysisofnon-...