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陝西省調查處理輸入性登革熱病例的報告
...。 1.2.2使用美國Cortez公司的DengueIgM/IgG硒標試劑、德國IBL公司的DengueIgGELISA試劑和中國疾病預防控制中心提供的登革熱病毒RNA引物,進行登革熱特異性IgM、IgG抗體和病毒RNA擴增檢測。 1.2.3媒介監測分別在陝西的西、中、東...
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海南省2005~2006年登革熱血清流行病學調查
...劑檢測登革IgM抗體,但2005年52份發熱病人血樣同時用德國IBL公司的ELISA試劑平行檢測登革IgM和IgG抗體。 1.3數據分析個案調查資料和實驗室結果錄入SPSS11.5進行統計分析。 2結果0.05),既往高發區總的登革IgG抗體陽性率爲1.9...
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Zincandpneumonia
...esult,noapparentexplanationwasforthcoming.Apharmacologiceffectofzincisplausible(5),but,asinthereportbyBoseetal,itiswidelyacceptedthatthebeneficialeffectsofzincsupplementsinthepreventionandtreatmentofdiarrheaandpneumoniaaremostlikelytobeduetothepreventionorcorrectionofzincdeficiency.Hence,beneficiale...
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UpregulatedExpressionof14-3-3ProteinsinAstrocytesFromHumanCerebrovascularIschemicLesions
...d1:400),arabbitpolyclonalanti-14-3-3antibody(Immuno-BiologicalLaboratories[IBL];diluted5μg/mL),andarabbitpolyclonalanti-14-3-3antibody(IBL;diluted2μg/mL).Theanti-14-3-3antibody(H-8)recognizesallhuman14-3-3isoforms,9,10whereastheotherantibodiesare14-3-3isoformspecific.10,11ImmunohistochemistryForma...
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肺結核患者血清和尿液新喋呤水平的變化
...並收集當天晨尿,保存於-20℃直至測定。NT測定採用德國IBL公司試劑盒(ELISA法),按照說明書操作步驟進行。爲排除尿液濃縮的影響,尿液NT水平以尿NT水平與尿肌酐(Cr)水平的比值表示。 1.3數據處理採用SPSS11.0軟件包。 2...
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肺炎衣原體慢性感染與冠心病的血清學相關性研究
...風溼因子(BF)吸附試驗後重新檢測(RF吸附液購自德國IBL公司)。 123診斷標準:①急性肺炎衣原體感染:雙份血清抗體(IgG或IgM)滴度升高4倍及其以上;IgM≥1∶16;IgG≥1∶512。上述3條符合其中1條即可診斷。②既往感...
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肺炎衣原體慢性感染與冠心病的血清學相關性研究
...風溼因子(BF)吸附試驗後重新檢測(RF吸附液購自德國IBL公司)。 123診斷標準: ①急性肺炎衣原體感染:雙份血清抗體(IgG或IgM)滴度升高4倍及其以上;IgM≥1∶16;IgG≥1∶512。上述3條符合其中1條即可診斷。②既...
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肺炎衣原體慢性感染與冠心病的血清學相關性研究
...風溼因子(BF)吸附試驗後重新檢測(RF吸附液購自德國IBL公司)。 123診斷標準:①急性肺炎衣原體感染:a雙份血清抗體(IgG或IgM)滴度升高4倍及其以上;b.IgM≥1∶16;c.IgG≥1∶512。上述3條符合其中1條即可診斷。②...
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深圳寶安區2~6歲兒童水痘疫苗免疫成功率監測
...所有標本隨機編號後,送寶安區CDC檢驗科檢驗,採用德國IBL公司的水痘-帶狀皰疹病毒IgG抗體檢測試劑及按照說明書操作步驟(ELISA法)檢測水痘IgG抗體,以8U/ml爲陰性,8U/ml爲陽性。 2結果 檢測147人,其中免疫前或後不合...
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2006年深圳市寶安區麻疹人羣免疫水平調查
...抗體濃度≥10ng/ml爲陽性,≤10ng/ml爲陰性。檢驗試劑德國IBL公司生產的MeaslesVirusIgGELISA試劑,批號爲RE56611,有效期爲2006年10月6日,在有效期內使用。2結果7個年齡組共監測352例,總陽性率爲84.09%。其中男181例,抗體陽性的有144例...
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ThyroidAutoimmunityandSpontaneousCervicalArteryDissection
...antidouble-strandeddeoxyribonucleicacidantibodiesbyradioimmunosorbentassay(IBL);antiextractablenuclearantigenantibodiesbyenzyme-linkedimmunosorbentassay(Innogenetics);rheumatoidfactorandC3toC4complementfractionbynephelometry(DadeBehring);andcryoglobulinsbystandardprocedure.Insubjectswithraisedlevels...
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不同年齡男性胃癌患者雄激素水平分析
...0。性激素結合球蛋白(SHBG)採用ELISA法測定,試劑盒系德國IBL公司產品,批內CV爲5.5,批間CV爲9.0。遊離睾酮根據Vermeulen公式計算,睾酮分泌指數(TSI)=總睾酮/LH(nmol/IU),遊離睾酮指數(FTI)=總睾酮/SHBG(nmol/nmo1)。標本的測定由同一檢測...
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EtiologyofMumps-LikeIllnessesinChildrenandAdolescentsVaccinatedforMeasles,Mumps,andRubella
...tyCentralHospital,HospitalforChildrenandAdolescents,Helsinki,FinlandThepossibleviraletiologyofmumps-likeillnessesinpatientsvaccinatedformeasles,mumps,andrubella(MMR)wasstudiedbyuseofserumsamplesprospectivelycollected,during19831998,from601acutelyillFinnishchildrenandadolescentswithmumps-likesymptoms...
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兒童風溼病患者肺炎支原體和EB病毒感染的研究
...IgA的測定全病毒EBV-IgM、IgA測定採用ELISA,試劑盒購自德國IBL公司(批號爲RE56211),操作步驟按常規進行,判斷標準CUTOFF爲陽性。 2結果 檢測結果見表1、表2、表3。 表1風溼病組與對照組血清MP-IgM、IgA,EBV-IgM、IgA陽性...
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降鈣素基因相關肽對大鼠急性肺損傷的治療作用及其可能機制
...碼】A【文章編號】1684-2030(2010)03-0129-04 Effectandpossiblemechanismofcalcitoningene-relatedpeptideonacutelunginjury LIBei-hua,LIUHui-lin,LIUTao.Hospitalof69250PLATroops,Urumchi830013,China 【Abstract】ObjectiveTostudytheprotectedeffectofcalcitoningene-relatedpeptide(...
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瑞芬太尼不同靶濃度與全血實測濃度的差異以及對血流動力學的影響
...,注入肝素抗凝管,離心後分離血清,-20℃保存,用德國IBL公司試劑盒,根據鄒遠高等[5]報道高效液相色譜-電化學法測定E和NE的含量。 1.5統計學方法採用SPSS10.0統計軟件包,計量資料以±s表示,二組間比較採用t檢驗,檢...
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Effectsofdietarysaturated,monounsaturated,andn3fattyacidsonbloodpressureinhealthysubjects
...unction,hemostaticfactors,andmicroalbuminuria.Geneticfactorsseemtoberesponsibleforasmuchas2040%ofBPvariationsinthegeneralpopulation(9).However,epidemiologicdataimplicatethatlifestylefactors(eg,dietaryhabits)areamajorcontributortothehighprevalenceofhypertension(10).Nevertheless,ourknowledgeofth...
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CharacterizationofMycobacteriumcapraeIsolatesfromEuropebyMycobacterialInterspersedRepetitiveUnitGenotyping
...ctivebandsizewascalculatedaccordingtoinformationprovidedbyPhilipSupply(www.ibl.fr/mirus/mirus.html)andenteredintoaspreadsheetfile.AtBorstel,MIRUcodesweredeterminedbyT.K.andS.N.bymultiplex-PCRMIRUtypingaccordingtoapreviouslydescribedmethod(45).SpoligotypingandIS6110RFLPanalysis.Spoligotypingwascarrie...
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中醫藥治療宮頸癌研究進展
...清掃加經陰道根治性宮頸切除尤其適宜有生育要求的Ia2~Ibl期且局部腫瘤直徑小於2cm的早期宮頸癌患者。但其中也存在着一些亟待解決的問題。諸如根治性子宮切除術可能會影響盆腔自主神經的功能甚至影響生殖及性功能,而盆...
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單核細胞激活及Th1/Th2細胞因子的變化與腎綜合徵出血熱病情的關係
...IL-12、IFN-γ均採用ELISA方法檢測,試劑盒分別購自德國IBL公司、比利時Biosource公司和法國Diaclone公司(IFNγ);IL-10用放射免疫法(RIA)檢測,試劑盒購自解放軍總醫院科技開發中心放免所。爲避免反覆凍融而影響檢測結果...
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Dietaryglycemicloadandriskofage-relatedcataract
...aselinesemiquantitativefood-frequencyquestionnaire(SFFQ),thosewhohadimplausiblecaloricintakes(600or3500kcal/dforwomenand800or4200kcal/dformen),andthosewholeft70itemsblankontheSFFQ;atotalof82126womenand49934menwerethuspotentiallyavailableforanalysis.Fromthesegroups,wefurtherexcludedallparticipantswho...
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EvidenceforaroleofproteinkinaseC-inurineconcentration
...usealbumin(CellTrend,Luckenwalde,Germany)oracommercialRIAkitforvasopressin(IBL,Hamburg,Germany),respectively.Afterurinecollectionwascompleted,themicewereanesthetizedwithetherandbloodwasdrawnfromtheretrobulbarplexusfordeterminationofhematocritandplasmaosmolality.Twenty-four-hoururinecollectionswerepe...
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RedistributionofvitaminAafterironsupplementationinIndonesianinfants
...f0.05andapowerof0.90.Toallowfordropouts,atargetof60infants/groupwasset.Eligibleinfantswereidentifiedbyvillagehealthvolunteers,andthemotherswereinvitedtoparticipateinthestudy.Motherswereinformedoftheproceduresandpurposeofthestudy.Afterwritteninformedconsentwasgivenbythemother,infantswereassessedanthr...
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AblationoftheSympatheticNervousSystemDecreasesGram-NegativeandIncreasesGram-PositiveBacterialDissemination:KeyRolesforTumorNecrosisFactor/Ph
...100,and1000cfuofP.aeruginosaorS.aureus(ATCC5638)/mL;10cfu/mLyieldedreproducibledatawiththesmallestcoefficientofvariation.Thisamountwasthenusedinallexperimentsshown(theuseofmorebacteriaresultedinbacterialovergrowthandthedestructionofcells).Supernatantwascollected,sodiumazidewasaddedtostopbacterialgro...
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DualMechanismsRegulatingAMPKKinaseActionintheIschemicHeart
...tingofancatalyticsubunitandandregulatorysubunits.TheprimarymechanismresponsibleforAMPKactivationinvolvesphosphorylationoftheThr172residuelocatedwithintheactivationloopofthecatalyticsubunit.9Additionalphosphorylationsiteshavebeenidentifiedontheandsubunits,buttheirfunctionalrolesremainuncertain.10,11A...
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Stimulationofsolubleguanylylcyclaseinhibitsmesangialcellproliferationandmatrixaccumulationinexperimentalglomerulonephritis
...asurementofcGMPwasperformedintriplicateusingacommercialradioimmunoassaykit(IBL,Hamburg,Germany)asdescribedbefore(33).Westernblotanalysis.Proteinsamplescontaining20μgofproteinfromisolatedglomeruliwereseparatedby8%SDS-PAGEandblottedontoPVDFmembranes.Blotswereblockedfor12hat4°Cinblockingsolutionconta...
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TheEffectofHydroxylamineonKATPChannelsinVascularSmoothMuscleandUnderlyingMechanisms
...esentericarteriesandunderlyingmechanisms.Itwasfoundthatbath-appliedHAreversiblyenhancedKATPcurrentsinaconcentration-dependentfashionwithanEC50of54±3.4eandhyperpolarizedthecellmembranefromeC48±5.2toeC65±7.5mV(n=6,p0.01).TheincreaseinKATPcurrentsinducedbyHAwassuppressedbysuperoxidedismutase(eC380±...
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Zincplusß-carotenesupplementationofpregnantwomenissuperiortoß-carotenesupplementationaloneinimprovingvitaminAstatusinbothmothers
...ingasinglehigh-doseofvitaminAtothemotherdirectlypostpartum.BecauseofthepossibleteratogenicityofvitaminAinhumans(7),theWorldHealthOrganizationrecommendsthatonlysmalldoses(10000IU/dor25000IU/wk)shouldbegiventomothersduringpregnancy(8).Similarly,onlysmalldoses(3dosesof50000IUwithintervalsofatleast1mo)o...
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AnalysisofMycobacteriumtuberculosisGenotypesinMadridandIdentificationofTwoNewFamiliesSpecifictoSpain-RelatedSettings
...ulatedbymeansofthereferencesincludedintheMIRUprovisionalwebpage(http://www.ibl.fr/mirus/mirus.html).TheMIRUtypeconsistsofa12-numbercodethatindicatesthenumberoftandemrepeatsfoundforeachoftheMIRUloci.PhylogeneticcomparisonsbetweengenotypeswereperformedusingBionumericsversion3.0(AppliedMaths,St.Marten-...
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Acyl-CoenzymeA:CholesterolAcyltransferase-2(ACAT-2)IsResponsibleforElevatedIntestinalACATActivityinDiabeticRats
...hatincontrolrats.Conclusions—OurdatademonstratethatACAT-2isozymeisresponsiblefortheincreasedintestinalACATactivityofdiabeticrats,suggestinganimportantroleofACAT-2fordyslipidemiaindiabeticpatients.Diabeticratsexhibitdyslipidemiacaused,inpart,byelevatedintestinalacyl-coenzymeA:cholesterolacyltransfe...