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南海海洋所揭示L-別異亮氨酸的生物合成機制
...Soc.(2016,138(1),408-415)發表文章首次闡述天然產物中L-allo-Ile結構單元的生物合成機制,論文被同期JACSSpotlights(2016,138,461)作爲研究亮點評述。該成果爲研究其它生物體中L-allo-Ile的來源和生物醫療提供了思路;“氨基轉移酶”和異...
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白細胞介素4受體α鏈基因多態性與支氣管哮喘的相關性研究
...440-44310(廣州)爲了檢測白細胞介素4受體α鏈(IL-4Rα)基因Ile50Val位點的多態性在漢族人羣中的分佈頻率,探討Ile50Val位點多態性與支氣管哮喘(簡稱哮喘)的相關性。研究者對2002至2004年間暨南大學附屬第一醫院呼吸科門診及住院患...
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SingleMutationsatAsn295andLeu305intheCytoplasmicHalfofTransmembrane-HelixDomain7oftheAT1ReceptorInducePromiscuousAgonistSpecificityfor
...cells—apseudo-constitutiveactivity.ItissurprisingthattheAngIIanalog[Sar1,Ile4,Ile8]AngIIandthenativeangiotensinIIfragmentsAng1-7,AngIV,andAng5-8,whichareinactiveinactivatingthewild-typereceptor,activatedN295SandL305Q.ResultsgeneratedbyloweringtheNa+concentrationsuggestthatthemutantN295SandL305Qmay...
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系統性紅斑狼瘡患者白細胞介素-4受體基因多態性的研究
...)爲了初步探討白細胞介素-4受體(IL-4R)的兩個氨基酸位點ile50val和gln576arg多態性與系統性紅斑狼瘡(SLE)患者發病的相關性。研究者採用聚合酶鏈反應-單鏈構象多態性(PCR-SSCP)和聚合酶鏈反應限制性片段多態性(PCR-RFLP)及DNA測序方法...
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磷脂酰肌醇3-激酶P85α亞單位基因Met326Ile變異與2型糖尿病的相關關係
...爲了探討磷脂酰肌醇3-激酶(PI3-K)基因P85α調節亞單位Met326Ile突變及基因表達與2型糖尿病(T2DM)的關係。研究者採用雙引物競爭PCR法檢測T2DM者240例及正常對照(NC)組150例Met326Ile突變,對部分受檢者的外周血白細胞RNA進行RT-PCR擴增,觀...
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STK15基因單核苷酸多態與大腸癌風險
...中華腫瘤雜誌2006Vol.28No.1P.43-467(北京)爲了探討STK15Phe31Ile基因單核苷酸多態與大腸癌風險的關係。研究者以聚合酶鏈反應(PCR)和限制性片段長度多態性(RFLP)分析方法,檢測了283例大腸癌患者和283例配對的正常對照者STK15Phe31Ile基...
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Thehumanorganicaniontransporter3(OAT3;SLC22A8):geneticvariationandfunctionalgenomics
...rogeneityamongOAT3variants,withthreevariants(p.Arg149Ser,p.Gln239Stop,andp.Ile260Arg)thatresultedincompletelossoffunction,andseveralotherswithsignificantlyreducedfunction.Oneofthemorecommonvariants(p.Ile305Phe),foundin3.5%ofAsian-Americans,appearedtohavealteredsubstratespecificity.Thisvariantexhibit...
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ActivationoftheAngiotensinIIType1ReceptorLeadstoMovementoftheSixthTransmembraneDomain:AnalysisbytheSubstitutedCysteineAccessibilityMetho
...monium(MTSEA).Thistreatmentledtoasignificantreductioninbindingof125I-[Sar1,Ile8]AngIItotheF249C,H256C,T260C,andV264Cmutantreceptors,suggestingthattheseresiduesorientthemselveswithinthewater-accessiblebindingpocketoftheAT1receptor.ItisnoteworthythatthispatternofacquiredMTSEAsensitivitywasalteredforTM...
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利用噬菌體肽庫確定IL-2Rα表位序列①
...守序列:Ser-Ser-Phe包含在4個序列爲Ser-Ser-Phe-Tyr-Pro(Leu)-Ile的噬菌體克隆中;Ser-Ser-Arg包含在10個序列爲Lys-Phe(Cys)-Ser-Ser-Arg-Leu(Lys)的噬菌體克隆中(見表2)。表2 DNA測序所得的氨基酸序列 Tab.2 AminoacidsequencesdeducedfromDNAsequencesNo...
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晚期結直腸癌GSTP1基因多態性與奧沙利鉑化療效果關係
【摘要】目的探討谷胱甘肽S轉移酶P1(GSTP1)基因Ile105Val(A→G)多態性與晚期結直腸癌對以奧沙利鉑爲主方案化療效果的關係。方法Ⅳ期結直腸癌病人102例,化療前取靜脈血應用TaqMan-MGB探針等位基因分型技術檢測GSTP1基因Ile105Val的多...
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內皮脂肪酶與血脂和冠心病的關係
...有潛在功能,其中有4個可引起氨基酸替換(Gly26Ser,Thr111Ile,Thr298Ser,Asn396Ser),其他2個變異在EL基因啓動子區域(-410C/G,-303A/C);Mank-Seymour等[18]在2004報道了13個新的EL基因單核苷酸變異,12個與HDL-C水平有顯著相關性,其中5個單核苷...
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Renalmedullarytissueoxygenationisdependentonbothcorticalandmedullarybloodflow
...itherthecortexormedulla.Inanesthetizedrabbits,renalarterialinfusionof[Phe2,Ile3,Orn8]-vasopressinselectivelyreducedmedullaryperfusionandPO2(54±24and50±10%,respectively)butdidnotsignificantlyaffectcorticalperfusionortissueoxygenation.Incontrast,stimulationoftherenalnervesresultedinrenalcorticalisch...
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藥物代謝酶CYP1家族相關基因的研究進展
...限制性內切酶MspI酶切後,可明確MspI多態性類型。1.1.2m2是Ile/Val多態性(CYP1A1*2C),又稱爲Exon7多態性。它也有三種基因型:野生型(Ile/Ile型),雜合型(Ile/Val型)及突變純合型(Val/Val型)三種形式。m2與m1位點突變高度關聯。1.1.3m3...
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單核苷酸多態性與靜脈血栓栓塞症的相關性分析
...,靜脈血栓栓塞症與Asp79His、Arg306The、Arg306Gly、Arg506Gln和Ile359The多態性基本無關;His1299Arg在VTE組中的分佈頻率高於對照組,但差異無統計學意義。Hisl299Arg與Metl736Ⅴal和As02194Gly多態性在人羣中有連鎖不平衡特性。深靜脈血栓和肺...
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一種現存的氨基酰-tRNA合成酶(AaLeuRS)具有祖先編校特性
...先編校特性的氨基酰-tRNA合成酶,揭示了AaLeuRS可能有着比IleRS及ValRS更爲原始的編校特性,其編校結構域可能保留了三種酶共同的祖先編校結構域,該發現爲研究氨基酰-tRNA合成酶催化特異性進化提供了的重要線索。氨基酰-tRNA合...
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3-2-5氨基酸的活化與轉運
...酸的活化過程。到目前爲止,另發現有二種氨基酸(Gln和Ile)的活化有其特殊條件。Gln-tRNA複合物的形成如下,即先由一個Glu經GluRS作用接在tRNAGln上,形成Glu-tRNA;然後再經轉氨基酶作用,將Glu(或Asn)的酰胺基接到Glu-tRNA複合物...
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一例乙型肝炎患者體內不同HBV克隆的序列差異
...並提出第三對等位基因亞型決定簇(i/t)的概念,即aa126位Ile爲i亞型,aa126位Thr爲t亞型,人們纔開始認識到這一位點在血清學分型中的重要性[10]。由於迄今已發表的所有adr亞型HBV在該位均爲Ile密碼子ATT,No.24患者感染的HBV顯然是一...
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ProteochemometricMappingoftheInteractionofOrganicCompoundswithMelanocortinReceptorSubtypes
...receptorsubtypesisadifficulttaskthatwouldbecomesimplifiedifonehadinhanddetailedknowledgeofthedeterminantsforligand-receptorinteraction.Wehaverecentlyinventedtheproteochemometricmodelingtechnologyfortheanalysisofprotein-ligandinteractions(Wikbergetal.,2003,2004).Thistechnologyisparticularlysuitedtoga...
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GSTP1在東北地區酒精性肝病發病機制中的研究
...[2]。是GSTs超基因家族中π家族的成員。GSTP1*D等位基因(Ile105-Val114)已被證實:具有Val105的酶比具有Ile105的酶對多環芳烴二醇環氧化作用的效力高7倍,GSTP1第5外顯子的一個位點的A→G突變導致兩個氨基酸的酶的活性部位被取代即...
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NeutralaminoacidtransportmediatedbyorthologofiminoacidtransporterSIT1/SLC6A20inopossumkidneycells
...transportresembles,butdoesnotmatch,thatdescribedformammalianB0AT1.UsingRNAsilencingtechniquesinOKcellsandX.laevisoocyteexpression,weshowherethattheB0-liketransportofOKcellsismediated,atleastinpart,bytheorthologofthemammalianIMINOsystemtransporterSIT1(SLC6A20).EXPERIMENTALPROCEDURESCellculture.Theest...
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Mass-SpectrometricIdentificationofaNovelAngiotensinPeptideinHumanPlasma
...LDI-TOF/TOF)revealedanangiotensinoctapeptidewiththesequenceAla-Arg-Val-Tyr-Ile-His-Pro-Phe,whichdiffersfromAngIIinAla1insteadofAsp1.Des[Asp1]-[Ala1]-AngII,inthefollowingnamedAngiotensinA(AngA),ismostlikelygeneratedenzymatically.Inthepresenceofmononuclearleukocytes,AngIIisconvertedtoAngAbydecarboxyla...
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鄰苯二甲醛柱前衍生反相高效液相色譜法測定水牛角中氨基酸的含量
...mnpackedwith5μmODS(octadecylsilance).AnacetatebufferatpH5.6wasusedasthemobilephaseA,whilemethanolwasemployedasthemobilephaseB.Lineargradientelutionwasadoptedwithafluorescentdetector(Ex=340 nm,Em=460 nm).Twelveaminoacidsinbuffalohornwereseparatedanddetectedin32min.Fromtheresults,agoodlinearrelat...
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關於食道癌相關基因的研究報告
...間的交互作用。在有抽菸嗜好的病人中,GSTM1null型和GSTP1ile/ile基因型有更高罹患食道癌風險。而飲酒且同時帶有GSTM1null和GSTP1val/val基因型,會造成中度的食道癌風險。GST基因型於職業上的暴露之間的交互作用,並不會影響食道癌...
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人可溶性IL-6R及其突變體基因在COS7細胞中的表達及活性分析①
...術擴增天然人sIL-6R基因片段,然後將第280位His殘基突變成Ile。天然基因和突變體基因分別轉染COS7細胞,經ELISA和Westernblot檢測證實轉染細胞上清中的表達產物,並利用BindingassayELISA和生物學功能分析法檢測表達產物與IL-6的結合能...
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IschemicNeoangiogenesisEnhancedby2-AdrenergicReceptorOverexpression
...doIaccarino,MicheleCiccarelli,DanielaSorriento,GennaroGalasso,AlfonsoCampanile,GaetanoSantulli,ErsiliaCipolletta,VincenzoCerullo,VincenzoCimini,GiovannaGiuseppinaAltobelli,FedericoPiscione,OrnellaPriante,LucioPastore,MassimoChiariello,FrancescoSalvatore,WalterJ.Koch,BrunoTrimarcotheDipartimentodiMed...
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BindingSiteofaNovelKv1.5Blocker:A“FootintheDoor“againstAtrialFibrillation
...tagenesisoftheporedomainofKv1.5identifiedtheaminoacidsThr479,Thr480,Val505,Ile508,Val512,andVal516asimportantresiduesforblockbyAVE0118.AhomologymodeloftheporeregionofKv1.5predictsthatthesesixresiduesfacetowardthecentralcavityofthechannel.Inaddition,mutationoftwootherS6residues(Ile502andLeu510)thatar...
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肽的溶解性及儲存
...極性氨基酸(疏水性)小的:Cys、Pro、Ala、Thr大的:Val、Ile、Leu、Met、Phe中性氨基酸大的:Trp、Tyr、His極性氨基酸(親水性)小的:Ser、Gly、Asp、Asn大的:Glu、Gln、Lys、Arg可溶性的問題許多合成的肽粗品,尤其是那些經過幾次沉...
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瘧原蟲抗藥性的遺傳學研究進展
...報道了6個DHFR基因編碼區變異:108位Ser→Asn或Thr,51位Asn→Ile,59位Cys→Arg,16位Ala→Val,164位Ile→Leu。單一點突變所致DHFR108位Ser變異成Asn即可產生乙胺嘧啶抗性,但對環氯胍的反應性僅稍有降低。對乙胺嘧啶產生高度抗性則需其他位...
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Thebeneficialeffectof-linolenicacidincoronaryarterydiseaseisnotquestionable
...talityfromCAD(P0.04)andfromallcauses(P0.02).TheintakeofALAinthehighestquintilewas3.2-foldthatinthelowestquintile.Morerecently,2largeprospectivestudiesin76 283nurses(4)and43 757healthprofessionals(5)showedthatALAwastheonlyfattyacidthatprotectedagainstcardiacdeath(4)andagainstnonfatalmyocard...
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急性呼吸窘迫綜合徵相關基因的研究
...R4(Asp299Gly)多態性進行分析,發現人TLR4基因的Asp299Gly和Thr399Ile多態性與吸入LPS後低應答相關,增加了由革蘭陰性菌引起的膿毒血癥的易感性。在這些人中,上皮細胞對LPS的刺激免疫應答能力減弱。進一步研究發現Asp299Gly比Thr399Ile更能...