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國內自身抗體檢測的現狀及質控分析
...tochondrialantibody,AMA)和抗平滑肌抗體(antismoothmuscleantibody,ASMA)。結果ANA、抗dsDNA抗體、抗ENA抗體、AMA和ASMA的檢測正確率分別是86.2%、75.4%、62.7%、100%和61.1%。可見與2002和2003年的質控相比,各項抗體檢測的正確率呈上升趨勢,但差...
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異基因造血幹細胞移植後自身抗體與cGVHD有關
...體包括抗核抗體(ANA)、抗線粒體抗體(AMA)、抗平滑肌抗體(ASMA)、抗心肌磷脂抗體(ACLA)、抗肝腎微粒體抗體(LKM)、抗DNA抗體、抗中性粒細胞胞質抗體(ANCA)及抗甲狀腺抗體,還檢測外周血表達抗CD3抗體、CD4抗體、CD8抗體、CD19抗體、CD20抗...
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IsolationofSidePopulationProgenitorCellsFromHealthyArteriesofAdultMice
...cubationwith10%serumofappropriatespeciesinPBS.Mouseanti--smoothmuscleactin(ASMA)(clone1A4;OncogeneResearchProducts,Boston,Mass),mouseanti-calponin(clonehCP;Sigma),ratanti-CD31(cloneMEC13.3),ratanti-VE-cadherin(clone11D4.1)(BDPharmingen),polyclonalrabbitanti-humanvonWillebrandfactorIg(DAKOCytomation)...
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CirculatingMatrixMetalloproteinase-9LevelsasaPossibleMarkerofAorticStiffness
...atartificiallyhigherMMP-9concentrationsarefoundinserumsamplescomparedwithplasmasamples.2–5Importantly,althoughahighcorrelationexistsbetweenMMP-9levelsinplasmaseparatedfromblooddrawnintotubeswithdifferentanticoagulants,nocorrelationwasfoundbetweenMMP-9levelsinserumandinplasma.Insummary,preanalytica...
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第二節 自身抗體的檢測及臨牀意義
...現假陽性。 (三)抗平滑肌抗體 抗平滑肌抗體(ASMA)產生機制不明,但與肝和膽管的自身免疫疾病有關,尤其是慢性活動性肝炎(狼瘡性肝炎)和原發性膽汁性肝硬化(PBC)。該類疾病的發生與自身免疫性密切相關,...
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Isdes-acylghrelincontributingtouremicanorexia?
...rsforhemodialysis-associatedappetitedisorders.Consistentevidenceshowsthatplasmaghrelinconcentrationsareincreasedinadvancedrenalfailure(4)andthatthosehigherconcentrationsdirectlycorrelatewiththeimpairmentofkidneyfunction,whichsuggeststhatreducedrenalexcretionofghrelinisthemaincausativefactorofghrelin...
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ReplytoDSKalmanandCMColker
...icksonD.Estimationoftheconcentrationoflow-densitylipoproteincholesterolinplasma,withoutuseofthepreparativeultracentrifuge.ClinChem1972;18:499502.SchaeferE,McNamaraJ.Overviewofthediagnosisandtreatmentoflipiddisorders.In:RifaiN,WarnickG,eds.Laboratorymeasurementoflipids,lipoproteinsandapolipopro...
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Evidence-basedmedicineandvitaminEsupplementation
...yfindingsfromthetrialperiodoftheATBCStudy.Insubjectsinthelowestquintileofplasma-tocopherolconcentration,thesimilarmortalityinthegroupswithsupplementintakesof50and0mg-tocopherol(n=1628and1610,respectively;seeTable3inreference2)refutesthenotionsthatalow-tocopherolintakeie,9.4mg/disthespeci...
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Disappearanceofeyelidxanthelasmafollowingoralsimvastatin(Zocor)
...cology.comAcceptedforpublication13September2004Keywords:simvastatin;xanthelasmaThemajorriskfactorsforcoronaryheartdiseaseincludesmoking,elevatedbloodpressure,andelevatedserumcholesterol.1Riskreductionstartswithidentificationofthoseatriskandthenalterationoffactorssuchasdiscontinuationofsmoking,loweri...
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Anewmethodfordeterminingplasmawatercontent:applicationinpseudohyponatremia
...aisaclinicalconditioncharacterizedbyanincreasedfractionofproteinorlipidinplasma,therebyresultinginanartificiallylowplasmasodiumconcentration([Na+]p).Sincetheautomatedmethodofmeasuring[Na+]pinmostlaboratoriesinvolvestheuseofanindirection-selectiveelectrode(I-ISE),thismethoddoesnotcorrectforelevatedpr...
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Zincdeficiencyinpatientswithsicklecelldisease
...rdiagnosingzincdeficiencyinpatientswithSCDincludeslowzincconcentrationsinplasma,erythrocytes,hair,lymphocytes,andgranulocytesandlowactivitiesofcertainzinc-dependentenzymes,suchascarbonicanhydraseinredbloodcells,alkalinephosphataseinneutrophils,andthymidinekinaseinnewlysynthesizingskinconnectivetissu...
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大陸地區引用次數最高的生物類論文
...ma,H.,...Experimentalandtheoreticalchallengesinthesearchforthequark-gluonplasma:TheSTARCollaboration‘scriticalassessmentoftheevidencefromRHICcollisions(Cited464time(s))2005;NuclearPhysicsA;Adams,J.,Aggarwal,M.M.,Ahammed,Z.,Amonett,J.,Anderson,B.D.,...Obesityandtheriskofmyocardialinfarctionin27000p...
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原發性肝癌中自身抗體的檢測
... 百分比(%)自身抗體類型佔陽性病例 百分比(%)HGG+NNG+ASMAAMAG+N+LMAH+MLMA總計全陰1712.2121000000410.5HBsAg+抗HBe+抗HBc+9870.541431201102668.4抗HBe+抗HBc+128.6120000001410.5抗HBs+抗HBe+抗HBc+42.901000100025.3抗HBs+32.210000000012.6HBsAg+HBeAg+抗HBe+抗HBc+21.40000000...
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Tacklingthegreatestchallengeincataractsurgery
...precedescapsularbagimplantationofaPMMAIOL.ThetechniqueiscommonlyabbreviatedasmanualSICS(smallincisioncataractsurgery).Anefficient,highvolumesystemutilisinglowcost,sub-5minuteprocedurestotackleadvancedcataractswithminimalcomplicationsisclearlythebestwaytousethescarcestandmostpreciousassetofthesystem...
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Influenceof10wkofsoyconsumptiononplasmaconcentrationsandexcretionofisoflavonoidsandongutmicroflorametabolisminhealthyadults
...whetherchronicsoyconsumptioninfluencesisoflavonebioavailabilityasjudgedbyplasmaisoflavoneconcentrationsandmodifiedgutmicrofloraactivities[ß-glucosidehydrolysisandequolandO-desmethylangolensin(O-DMA)production].Wealsoexaminedwhethersexdifferencesinisoflavonemetabolismexist.Design:Arandomized,par...
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Covalently-BoundHeparinMakesCollagenThromboresistant
...entorthroughbypasssurgery.Unfortunately,attemptstoimplantvasculargraftswithasmalldiameterarenotsuccessfulbecauseofthromboticandinflammatoryreactions.1–6Thus,toenhancebloodcompatibility,asurfacehastobedesignedthatisbothanti-immunogenicandthromboresistant.Collagenhasbeenwidelyusedinmedicalapplicatio...
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UniquePathwayforCholesterolUptakeinFatCells
...eapoE,awell-recognizedapoproteinthatinfluencestheprocessingandclearanceofplasmalipoproteincholesterolandalsoinfluencesintracellularcholesterolhomeostasisinseveralcelltypes.10Inthecurrentstudy,theauthorshavepainstakinglydefinedthemechanismsforthisSR-BI–independentCEselectiveuptakefromHDLinadipocyte...
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Lipidmetabolismduringenduranceexercise
...ing-inducedincreaseinfatoxidationisdueprimarilytoincreasedoxidationofnon-plasma-derivedfattyacids,perhapsfromintramusculartriacylglycerolstores.Fatoxidationislowerinhigh-intensityexercisethaninmoderate-intensityexercise,inpartbecauseofdecreasedfattyaciddeliverytoexercisingmuscles.Parenterallipidsupp...
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Lowerplasma-carboxyethyl-hydroxychromanafterdeuterium-labeled-tocopherolsupplementationsuggestsdecreasedvitaminEmetabolisminsmokers
...othesizedthatsmokerswouldhaveless-tocopherolavailableandconsequentlylowerplasma-carboxyethyl-hydroxychroman(-CEHC),the-tocopherolmetaboliteproducedbyacytochromeP450mediatedprocess.Design:Smokersandnonsmokers(n=10pergroup)weresupplementedwithdeuterium-labeled-tocopherylacetates(75mgeachd3-RRR--...
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男性不育患者免疫因素AsAb與TNF-α的相關性研究
...,Wuhu241001.【Abstract】ObjectiveTostudytherelationshipbetweenseminalplasmaandserumofTNF-inmaleinfertilˉity.MethodswedetectedTNF-αconcentrationsinseminalandserumplasmathroughradioimmunoassay;anddeˉtectedAsAbinseminalandserumplasamathroughELISA.Results(1)TheTNF-αconcentrationsinseminalpla...
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Smokingandexposuretoenvironmentaltobaccosmokedecreasesomeplasmaantioxidantsandincrease-tocopherolinvivoafteradjustmentfordietaryantioxidan
...etomacromolecules,contributingtocardiovasculardiseasesandcancer.Decreasedplasmaantioxidantconcentrationsmayindicatecigarettesmokerelatedoxidativestress.Objective:Wecomparedtheeffectsonplasmaantioxidantconcentrationsincotinine-confirmedactiveandpassivesmokerswiththoseinnonsmokers,independentofd...
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AdenoviralTransferofEndothelialNitricOxideSynthaseAttenuatesLesionFormationinaNovelMurineModelofPostangioplastyRestenosis
...olesterolemia,tendstobehypercellular,highlyenrichedinsmoothmusclecellactin(ASMA)content,andrelativelydevoidofextracellularmatrixcomponents.13–15Inclinicalpractice,however,atheroscleroticlesionsformthelipid-richandinflammatory“soil“ofrestenosis,whichisreflectedhistologicallybythepresenceoffoamc...
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Atrialnatriureticpeptide:anessentialphysiologicalregulatoroftransvascularfluid,proteintransport,andplasmavolume
...s,California,USA.AbstractAtrialnatriureticpeptide(ANP)actsacutelytoreduceplasmavolumebyatleast3mechanisms:increasedrenalexcretionofsaltandwater,vasodilation,andincreasedvascularpermeability.AuthorsofastudyinthisissueoftheJCIperformedaknockoutofthereceptorforANPinvascularendotheliainordertodistinguis...
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BiologicallyActiveCD40LigandIsElevatedinSickleCellAnemia
...entiallyplaysaroleinSCA.MethodsandResults-ELISAsdemonstratethatSCApatientplasmacontains30-foldmoresCD40Lthancontrolplasma.Correspondingly,plateletsfromthesepatientscontainlessthanhalftheCD40Lfoundincontrolplatelets.PlateletsfrompatientsinpainfulcrisesarefurtherdepletedofCD40L,withevenhigherplasmalev...
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不孕婦女β-內啡肽與生殖激素水平及其與精神心理因素關係的探討
...sweremeasured,andtheovulationmonitoredsimultraneously.Results(1)Theplasmalevelofβ-EPininfertilegroupandcontrolwerelowestinmid-follicularphase,elevatedalittleinperiovulatoryphaseandraisedsignificantlyinmid-lutealphase(P0.05),displayingaascendingtrendincycles.Ininfertilegroupplasmaβ-EP...
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S-Adenosylhomocysteine—abetterindicatorofvasculardiseasethanhomocysteine?
...il:conrad.wagner{at}vanderbilt.edu.ABSTRACTItiswidelyacceptedthatelevatedplasmatotalhomocysteineisanindependentriskfactorforvasculardisease.Therelationisbelievedtobecausal,butthereisnogenerallyacceptedmechanismforthepathophysiologyinvolved.ThemetabolicprecursorofhomocysteineinalltissuesisS-adenosylh...
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Two-photoninvivomicroscopyofsulfonefluoresceinsecretioninnormalandcysticratkidneys
...an‘scapsuleaveraged0.15±0.04(n=17)timesthatofglomerularcapillaryplasma,consistentwithextensiveplasmaproteinbindingofSF.InnormalHan:SPRDrats,steady-statecellcytoplasmSFfluorescenceinproximaltubuleanddistaltubulecellsaveraged,respectively,2.7±1.4(n=99tubules)and0.2±0.2(n=17)time...
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老年人冠心病發病與血漿同型半胱氨酸水平相關性研究
...。【參考文獻】1UelandPM,RefsumH,StablerSP,etal.Totalhomocysteineinplasmaofserum:Methodsandclinicalapplications.ClinChem,1993,39(9):1764-1769.2JacobsenDW,GatautisVJ,GreenR,etal.RapidHPLCdeterminationoftatalhomocysteineandotherthiofinserumandplasma:sexdifferentandcorrelationwithcobafaminand...
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IdentificationandCharacterizationofClinicalIsolatesofMembersoftheStaphylococcussciuriGroup
...2strains).Staphylocoagulase(freecoagulase)activitywasdeterminedwithrabbitplasma(Torlak,Belgrade,Serbia)byusingthetubemethod(5).Oxidaseactivitywasdeterminedwithoxidasediagnostictablets(Rosco,Taastrup,Denmark).NovobiocinsusceptibilitywasdeterminedonMueller-Hintonagar(OxoidLimited,Basingstoke,Hampshire...
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第四章 受體的術前評估和治療--第一節 受體的術前評估
...抗體(ANA);④抗線粒體抗體(AMA);⑤抗平滑肌抗體(ASMA);⑥EB病毒抗體;⑦鉅細胞病毒(CMV)抗體;⑧甲胎蛋白(AFP)和癌胚抗原(CEA);⑨人類免疫缺陷病毒(HIV)。 對於Budd-Chiari綜合徵、酒精性肝硬化及其他原因...