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DuplexReverseTranscription-PCRFollowedbyNestedPCRAssaysforDetectionandIdentificationofBrazilianAlphavirusesandFlaviviruses
...irus(PIXV,subtypeIV),Mayarovirus(MAYV),Unavirus,andflavivirusesdenguevirus(DENV,subtypes1to4),SaintLouisencephalitisvirus(SLEV),Bussuquaravirus(BSQV),Cacipacorevirus,Iguapevirus,Ilheusvirus(ILHV),Rociovirus(ROCV),andyellowfevervirus(YFV)havebeenisolatedfrommosquitoes,animals,orhumans(1,15,37).Thesea...
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FlowCytometry-BasedAssayforTitratingDengueVirus
...rolina,ChapelHill,NorthCarolinaABSTRACTPlaqueassaysfortitratingdenguevirus(DENV)aretime-consumingandnotsuitableforstrainsthatdonotplaque.Fluorescence-activatedcellsorting(FACS)hasbeenusedtodetectDENV-infectedcells.HerewedescribeaFACS-basedassayfortitratingDENV.Wedeterminedthatat24hpostinfection,then...
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Nature:登革熱病毒的感染能力
...其他地方是一個新出現的威脅。它是由四種登革熱病毒(DENV-1、2、3和4)中的其中一種引起的,由斑蚊傳播;目前沒有得到批准的疫苗,也沒有有效的特效療法。登革熱病毒是小型黃病毒,很可能需要大量宿主因子才能傳播,因...
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ImprovedMolecularDetectionofDengueVirusSerotype1Variants
...51,1992)isusedworldwidefordiagnosisandsurveillance.However,itfailedtodetectDENV-1variantsinCambodiaduetoapointmutation.WedescribeanimprovementofthemethodthatallowsthedetectionofadditionalDENV-1strains,includingpotentialvariants. TEXTTopAbstractTextReferencesDenguevirus(Flaviviridae,genusFlavivirus...
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ComparativeAnalysisofImmunoglobulinM(IgM)CaptureEnzyme-LinkedImmunosorbentAssayUsingVirus-LikeParticlesorVirus-InfectedMouseBrainAntigensTo
...s,wehavedevelopedaeukaryoticplasmidvectortoexpressthepremembrane/membraneandenvelopeproteinswhichself-assembleintononinfectiousvirus-likeparticles(VLPs).InadditiontotheplasmidsforJapaneseencephalitisvirus,WestNilevirus(WNV),St.Louisencephalitisvirus(SLEV),anddenguevirustype2(DENV-2)reportedearlier,w...
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福州市2004年登革熱流行病學和病原學特徵分析
...病毒基因的核苷酸序列比對證明同源性與柬埔寨分離株(DENV-1/KHM/2001,GenBankAccessionNo.L0904278)最爲接近。可見福州市2004年登革熱疫情爲感染登革1型病毒所引起;疫情的發生主要爲輸入性病例流行引起。作者:
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登革熱流行嚴重程度的關鍵因素被確定
...次的登革熱感染——當其第二次感染了該病毒的一種叫做DENV-2的亞組時卻會死於該病的出血熱形式。文章的作者先前對2004-2005年及2008-2009年在尼加拉瓜的馬那瓜的登革熱流行開展了兩項獨立的臨牀研究。該團隊用來自這些試驗的...
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Occasionalphysicalinactivitycombinedwithahigh-fatdietmaybeimportantinthedevelopmentandmaintenanceofobesityinhumansubjects
...cineandBiometrics,andofPediatrics,UniversityofColoradoHealthSciencesCenter,Denver.2SupportedbygrantDK-46881fromtheNationalInstituteofDiabetesandDigestiveandKidneyDiseases,grantP30DK-48520-01fromtheColoradoClinicalNutritionResearchUnit(MetabolicandEnergyBalanceLaboratories),andgrantMO1-RR00051fromthe...
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HerpesSimplexVirusType2InfectionasaRiskFactorforHumanImmunodeficiencyVirusAcquisitioninMenWhoHaveSexwithMen
...icine,2Epidemiology,and3LaboratoryMedicine,UniversityofWashington,Seattle;4DenverDepartmentofPublicHealth,Denver,Colorado;5AIDSOffice,SanFranciscoDepartmentofPublicHealth,SanFrancisco,California;6LaboratoryofEpidemiology,NewYorkBloodCenter,NewYork;7DepartmentofPsychology,UniversityofIllinoisandHowar...
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ACatalyticAntioxidantAttenuatesAlveolarStructuralRemodelinginBronchopulmonaryDysplasia
...searchCenter;DepartmentofMedicine,UniversityofColoradoHealthSciencesCenter,Denver,Colorado;DepartmentofPediatrics,UniversityofTexasHealthSciencesCenter;SouthwestFoundationforBiomedicalResearch,SanAntonio,Texas;DepartmentofPathology,HarvardMedicalSchool;DepartmentsofPathology,Children‘sHospital;and...
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職業睡眠學會第19屆年會熱點(2005-6)
...nnualMeeting2005年6月18-23日美國科羅拉多州丹佛June18-23,2005,Denver,ColoradoAdvancesinUnderstandingtheNatureofInsomniaThomasRoth,PhDTheAssociationofProfessionalSleepSocieties(APSS)heldits19thannualmeetingonJune16-23,2005,inDenver,Colorado.Insomniawasamajorfocusatthisyear‘sconference;...