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肝硬化並反流性食管炎的臨牀、內鏡及病理分析
...色檢查,亦按1999年煙臺標準進行分級。鱗狀上皮增生、炎細胞浸潤爲輕度,出現黏膜糜爛爲中度,有潰瘍形成或Barrett食管改變爲重度。 1.4統計學處理採用χ2檢驗。 2結果 2.1內鏡檢查結果RE內鏡下表現爲黏膜點狀或條狀...
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核因子κB的結構和功能與內毒素介導的肝損傷的關係
...ppaB(NF-κB)是近年來研究極爲廣泛的核內轉錄調控因子,在細胞炎性反應、免疫反應以及細胞凋亡等過程中起着舉足輕重的作用。NF-κB參與多種細胞因子及急性期反應蛋白轉錄活性的調控,在免疫和炎性疾病的啓動中起重要作用。...
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GeneticDiversityandRecombinationofPorcineSapoviruses
...manstrainsexist.INTRODUCTIONSapoviruses(SaV),previouslyreferredtoasSapporo-likeviruses,areemergingentericpathogensthatcausediarrheainhumans,pigs,andmink(5,7,9,10,22).Theyarenonenveloped,polyadenylatedsingle-strandedpositive-senseRNAvirusesandbelongtotheSapovirusgenusinthefamilyCaliciviridae(7).TheSa...
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SensitiveEDTA-BasedMicrobiologicalAssaysforDetectionofMetallo--LactamasesinNonfermentativeGram-NegativeBacteria
...othecrudeextracts10minbeforeperformingtheassay.ThepresenceofthegenesblaVIM-like,blaIMP-like,andblaSPM-1(forbothPseudomonasspp.andA.baumannii)andblaL1-like,blaIND-like,andblaGOB-like(forStenotrophomonasmaltophilia,Chryseobacteriumindologenes,andChryseobacteriummeningosepticum,respectively)wasanalyzed...
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一種新型的polo-like激酶途徑調節劑ON01910.Na在成人實體瘤治療的I期臨牀試驗
SOURCE:JournalofClinicalOncology一種新型的polo-like激酶途徑調節劑ON01910.Na對成人實體瘤患者進行了I期臨牀試驗,旨在確定其劑量限制性毒性(dose-limitingtoxicities,DLTs)。20例(11女,9男)難治性(針對常規治療)實體瘤患者納入研究,...
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肺炎患兒心肌損害205例分析
...2)缺氧、酸鹼平衡失調及細菌、病毒等毒素均可致心肌細胞損害而引起ST-T改變、Q—T間期延長及各種異位搏動的發生;(3)缺氧導致肺動脈高壓,使右心負荷加重,在心電圖上可表現爲P波高尖、不完全性右束支傳導阻滯;(4)病...
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DevelopmentofaMultiplexPCRandSHVMelting-CurveMutationDetectionSystemforDetectionofSomeSHVandCTX-M-LactamasesofEscherichiacoli,Klebsiella
...mentofanovelsystem,whichconsistsofamultiplexPCRtoidentifyblaSHV,blaCTX-M-3-like,andblaCTX-M-14-likegenesandamodifiedSHVmelting-curvemutationdetectionmethodtorapidlydistinguishsixprevalentblaSHVgenes(blaSHV-1,blaSHV-2,blaSHV-2a,blaSHV-5,blaSHV-11,andblaSHV-12)inTaiwan.Sixty-fiveclinicalisolates,which...
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內毒素激活巨噬細胞的信號傳導
...通過與膜受體CD14作用將信號從胞外傳到胞核而刺激多種細胞合成和釋放TNF-α、IL-1和IL-6等生物活性物質而表現複雜的生物學活性,但其詳細機制尚未完全清楚。本文就近年來對LPS激活巨噬細胞的信號傳導機制的研究作一簡要綜...
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VacciniavirusproteinA46RtargetsmultipleToll-like–interleukin-1receptoradaptorsandcontributestovirulence
...tkeyaspectsofthehostantiviralresponse.Recently,ithasbeenrecognizedthatToll-likereceptors(TLRs)havearoleininnatedefenseagainstviruses.Here,wedefinethefunctionofthevacciniavirus(VV)proteinA46RandshowitinhibitsintracellularsignallingbyarangeofTLRs.TLRsignallingistriggeredbyhomotypicinteractionsbetweent...
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MolecularCharacterizationsofHumanandAnimalGroupARotavirusesinTheNetherlands
...peG8wasnotfound.GenotypeG6bovinerotavirusesweredividedintothreeclusters:UK-like,VMRI-29-like,andHun4-like.DNAsequencingofapartoftheVP7genewasshowntobeusefulasaquickdeterminationofuncommonornovelstrainsofwhichthegenotypingcannotbedonebygenotypingPCR.Ofequinestrains,bothVP4andVP7genescouldbeusedforgen...
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補體系統複習題
...A.C2aB.C3aC.C3bD.C5aE.Ba6.補體不具備下列哪種作用?A.溶菌和細胞毒作用B.中和毒素作用C.調理作用D.炎症介質作用E.免疫調節作用7.具有趨化作用的補體組分是A.C2aB.C3bC.BaD.C4aE.C5a8.具有免疫粘附作用的物質A.C2aB.C2bC.C3aD.C3bE.C4a9.補體經典...
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SerumAmyloidAInducesContraryImmuneResponsesviaFormylPeptideReceptor-Like1inHumanMonocytes
...rTNF-andIL-10production,respectively.Thestimulationofformylpeptidereceptor-like-1-expressingRBL-2H3cells,butnotofvector-expressingRBL-2H3cellswithserumamyloidA,inducedmitogen-activatedproteinkinasesactivationandtheaccumulationoftheRNAsofthesetwocytokines.Together,ourfindingssuggestthatserumamyloidAm...
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Opioidreceptor-like1stimulationinthecollectingductinducesaquaresisthroughvasopressin-independentaquaporin-2downregulation
...ociceptin,theendogenousligandoftheinhibitoryGprotein-coupledopioidreceptor-like1receptor,producesaquaresis(i.e.,increasestheexcretionofsolute-freeurine)inrats.However,themechanismunderlyingthiseffecthasnotyetbeenexplained.Usingimmunohistochemistry,wefoundtheopioidreceptor-like1receptorintheratkidney...
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Community-WideOutbreaksofClonallyRelatedCTX-M-14-Lactamase-ProducingEscherichiacoliStrainsintheCalgaryHealthRegion
...57(15%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-Igroup(CTX-M-1-like)and87of157(55%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-IIIgroup(CTX-M-14-like).Theobjectiveofthisstudywastoinvestigatethemolecularepidemiologyofthesestrains.The-lactamaseswerecharacterized,andthegeneticrelat...
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腸產志賀樣毒素的大腸桿菌菌株中小腸結腸炎耶爾森菌毒力島基因irp-2的檢測
...進化的一個重要的推動力量〔1,2〕。另外,毒力島和宿主細胞的G+CMol%、密碼使用的明顯差異,也提示毒力島可能是外源性的。目前認爲水平性的基因轉移可能會導致新的病原性細菌的產生。毒力島的發現,使這一學說得到了廣...
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毒力島和細菌毒力的進化
...ol%、密碼使用和宿主細菌染色體有明顯差異,有的比宿主細胞的G+Cmol%明顯高,有的明顯低;5.毒力島編碼的基因產物許多是分泌性蛋白和細胞表面蛋白,如溶血素、菌毛和血紅素結合因子,一些毒力島編碼細菌的分泌系統(如Ⅲ...
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MolecularEpidemiologyofNorovirusInfectionsinStockholm,Sweden,duringtheYears2000to2003:AssociationoftheGGIIbGeneticClusterwithInfectionin
...evel(genogroupII).Twenty-nineoutof39(74%)oftheRLB-typedspecimenswereGGII-4-like.TheGGII-positivespecimensthatcouldnotbefurthertypedwerefrom36childrenand11adultpatients.Only5outof41specimensfromchildrenmatchedtheRLBpanel,andofthose,4wereGGII-4-likeandonewasGGI-6-like.Thelastoriginatedfromafood-borneo...
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腸出血性大腸埃希氏菌的多重PCR檢測方法
...醫學細菌學重點實驗室)關鍵詞:大腸桿菌;溶血素類;毒素類;聚合酶鏈反應 【摘要】 目的 開發腸出血性大腸埃希氏菌(entero-hemorrhagicE.coli,EHEC)的多重PCR檢測方法。方法 以溶血素(hemolysin,hly)基因和志賀樣毒素(Shigaliket...
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致瀉性大腸桿菌中發現小腸結腸炎耶爾森菌毒力島
...類菌能同時與Inv、SLT1或SLT2探針雜交呈陽性反應,對Hep-2細胞呈集聚性粘附,但不與EAggEC特異性探針反應,且不具有EIEC及志賀氏菌的ipaB基因〔2〕。 近年來,在醫學細菌學領域出現了一個新名詞——毒力島(pathogenicityisland)...
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AnIGF-Iantagonistdoesnotinhibitrenalfibrosisintheratfollowingsubtotalnephrectomy
...Biotechnology,UniversityofSheffield,Sheffield,UnitedKingdomABSTRACTInsulin-likegrowthfactorI(IGF-I)hasbeenproposedasamediatorofkidneyscarring,althoughnointerventionalstudiesontheroleofIGF-Iinmodelsofchronickidneydiseasehavebeenreported.TheeffectofapeptideIGF-Ireceptorantagonist(JB3)hasbeenexaminedon...
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MolecularEpidemiologyofCalicivirusesDetectedinSporadicandOutbreakCasesofGastroenteritisinFrancefromDecember1998toFebruary2004
...hichhadnotbeendetectedbeforeinourlaboratory:thenewvariant,GGIIb,andNorwalk-likestrains,whichweredifficulttodetect,in2000aswellasanewvariantLordsdalestrainin2002.Althoughthesestrainsemergedduringaperiod,othergenotypesstillcocirculated.Amongthesethreegroupsofstrains,thenewvariant,GGIIb,andthenewLordsd...
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尋找藥物設計的新靶點
...來有關研究的概況。 1 靶向CD分子的治療製劑 白細胞分化抗原(CD分子)是白細胞在正常分化過程的不同階段出現或消失的細胞表面分子,它們大多是蛋白質或糖蛋白,一般可分爲胞外區、穿膜區和胞內區。目前CD分子共有...
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NucleicacidsofmammalianorigincanactasendogenousligandsforToll-likereceptorsandmaypromotesystemiclupuserythematosus
...FN-.Wehavedevelopedthreeseriesofoligonucleotide(ODN)-basedinhibitorsofToll-likereceptor(TLR)signaling.TheseODNsincludeinhibitorsofTLR9,inhibitorsofTLR7butnotTLR9,andsequencesthatinhibitbothTLR7andTLR9.SpecificityoftheseinhibitorsisconfirmedbyinhibitionofIFN-productionbyPDCsinresponsetoDNAorRNAviruse...
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核因子-kappaB與急性肺損傷
...一具有多功能轉錄與調節作用的蛋白質因子,它能與多種細胞因子和粘附因子基因啓動子部位發生結合,增強這些基因的轉錄和表達,還可能延緩PMN的凋亡,從而廣泛參與基因轉錄的調節,特別是一些與機體免疫應答反映密切相...
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腸缺血再灌注損傷防治研究進展
...起網狀內皮系統發生系列反應,進而導致大量相關介質和細胞因子的釋放,甚至發生多系統器官功能不全綜合徵[1]。因此近年來越來越受到重視,其發生發展機理及防治措施的研究也成爲外科領域的重點課題之一。 腸I/R...
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DefinitionoftargetantigensfornaturallyoccurringCD4+CD25+regulatoryTcells
...dsencodingthefollowingSEREX-definedself-antigens:MusheatshockproteinDnaJ–like2(DnaJ–like2;AF055664),MusDNAligase1(Ligase1;U19604),Musgalectin-8(Galectin-8;AF218069),orMuspoly(A)bindingprotein,cytoplasmic1(Poly(A);X65553).1wkafterimmunization,splenicCD4+CD25+TcellsandCD4+CD25–Tcellswereisolated...
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冠心病與免疫
脂紋內皮下脂質過度堆積的巨噬細胞泡沫細胞,fattystreakT淋巴細胞10歲見於主動脈,20歲見於冠脈,30歲見於腦動脈發展早期病竈中心壞死,含細胞碎片、膽固醇、炎症細胞、巨噬細胞泡沫細胞,由平滑肌細胞、細胞外基質,內表...
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TLR4易化細菌在腎集合管細胞中的遷移
...表了一篇文章,認爲致腎盂腎炎大腸桿菌在腎髓質集合管細胞的移位和定植與TLR4易化的脂筏功能相關。致腎盂腎炎大腸桿菌(UropathogenicEscherichiacoli,UPEC)是最常見的尿路感染和腎盂腎炎的致病菌,腎髓質集合管細胞是UPEC定植...
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上海生科院通過單細胞RNA測序對軀體感覺神經元重新分類
圖A.熱圖顯示了197個神經元依據細胞大小排列的層次聚類的矩陣。10個族羣通過黑線方框標註,族羣的細胞大小顯示在左側。圖B.背根神經節初級感覺神經元分類示意圖。依據單細胞測序和原位雜交數據建議其類別和比例,依據電...
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TempolreducesoxidativestressandrestoresrenaldopamineD1-likereceptor-Gproteincouplingandfunctioninhyperglycemicrats
【摘要】DopamineviaactivationofrenalD1-likereceptorsinhibitstheactivitiesofNa-K-ATPaseandNa/Hexchangerandsubsequentlyincreasessodiumexcretion.Decreasedrenaldopamineproductionandsodiumexcretionareassociatedwithhyperglycemicconditions.WehaveearlierreportedD1-likereceptor-Gproteinuncouplingandreduc...