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實時熒光定量PCR法檢測人骨肉瘤耐MTX細胞系中RFC、DHFR、GST-π的mRNA表達
【摘要】[目的]研究RFC(還原葉酸載體)、GST-π(谷胱甘肽S轉移酶π)、DHFR(二氫葉酸還原酶)mRNA在人骨肉瘤U2-OS細胞系及人骨肉瘤耐藥細胞系U2-OS/R1-R3中的表達差異,並探討其在人骨肉瘤MTX化療耐藥中的意義。[方法]採用衝...
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昆明動物所葉酸代謝與神經嵴發育研究取得新進展
...課題組的博士研究生李傑晶等人發現,還原性葉酸載體(RFC)在非洲爪蛙神經嵴發育中具有重要功能。RFC在非洲爪蛙神經嵴組織中特異性表達,干擾RFC的功能會抑制神經嵴的發生,而過表達RFC或注射5-甲基四氫葉酸可以促進神經...
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中醫藥逆轉類風溼多藥耐藥思路
...進入細胞通過兩種途徑:葉酸鹽受體和還原葉酸鹽載體(RFC)。葉酸鹽受體對葉酸的親和力比對還原葉酸的親和力要高,RFC對還原葉酸的親和力相對較高。甲氨喋呤因其結構與生理性葉酸鹽相同,主要通過RFC轉運入細胞內,因此...
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Interactionsamongpolymorphismsinfolate-metabolizinggenesandserumtotalhomocysteineconcentrationsinahealthyelderlypopulation
...izinggenes:methylenetetrahydrofolatereductase(MTHFR),reducedfolatecarrier1(RFC1),andglutamatecarboxypeptidaseII(GCPII).Design:Healthyvolunteers(436menand606women;meanage:77.9y)wererandomlyselectedfromamongresidentsofOxford,UnitedKingdom.WedeterminedtheindividualeffectsandinteractionsoftheMTHFR677CT,...
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妊娠滋養細胞疾病的實驗研究進展
...19]應用基因芯片技術,發現複製因子C(replicationfactorC,RFC)在葡萄胎組織和絨癌中表達水平顯著高於正常胎盤組織和絨毛細胞。結合免疫組化SP法檢測正常絨毛、葡萄胎、侵蝕性葡萄胎和絨癌組織中RFC2和PCNA(增殖細胞核抗原...
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Homocysteineconcentrationsinadultswithtrisomy21:effectofBvitaminsandgeneticpolymorphisms
...homocysteinepolymorphismCBSMTRMTRRMTHFRRFCvitaminB-12vitaminB-6INTRODUCTIONDownsyndrome(MIM190685),ortrisomy21,isacomplexmetabolicandgeneticdisorderthatstemsfromthefailureofchromosome21tosegregatenormallyduringmeiosis(1).Morethan80clinicalfeatureshaveb...
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雷貝拉唑三聯方案治療Hp陽性胃炎96例
...法對照組Hp陽性胃炎患者服用雷貝拉唑爲主新三聯方案即RFC方案(雷貝拉唑10mg、呋喃唑酮0.1g、克拉黴素500mg,均每日2次)共7天;對照組採用國產奧美拉唑爲主的三聯方案,即OMA方案(奧美拉唑20mg、甲硝唑0.4g、阿莫西林1.0g,均每日2...
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TheReducedFolateCarrierGeneIsaNovelSelectableMarkerforRecombinantProteinOverexpression
...olateauxotrophsthattakeupfolatevitaminsprimarilyviathereducedfolatecarrier(RFC).Inthisstudy,weshowedthatthehumanRFC(hRFC)genecanserveasanovelselectablemarkerfortheoverproductionofrecombinantproteins.Towardthisend,ahemagglutinin(HA)epitopetaggedhRFC(hRFC-HA)wasintroducedintoabicistronicvector(pIRES2-...
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楊寶峯校長會見韓國代表團
...峯校長受孫堯副省長委託親切會見了韓國RNLBIO株式會社和RFC三味食品研究所代表團一行六人,省醫學科學院金連弘院長、袁重勝副院長、省外事辦韓朝處楊鴻鵬處長等陪同會見。楊校長和金院長向客人詳盡地介紹了我校的歷史、...
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維生素B1缺乏是造成糖尿病人血管損傷的原因
...酶來間接測量維生素B1濃度,而這一酶的活性由於THTR-1和RFC-1蛋白維持正常,因此科學家一直沒有發現維生素B1的下降。科學家發現血管細胞中維生素B1的減少和微血管併發症密切相關,這似乎和內皮細胞有關,並且會使得患...
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2008年度“中國高等學校十大科技進展”揭曉
...布) ⑤清華大學 下一代互聯網核心技術國際標準RFC5210 (IPv6真實源地址體系結構及測試牀) 當前互聯網體系結構對分組的源地址不進行驗證,帶來了網絡安全、管理和計費等多方面的問題。清華大學吳建平團隊...
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骨肉瘤治療的研究進展
...後不良的主要原因,運輸MTX跨膜入胞的還原性葉酸載體(RFC)成爲腫瘤耐藥的新視點。Hattinger等[8]在MTX耐藥的變種Saos-2細胞系研究中發現RFC基因表達顯著減小,並指出RFC基因表達量與MTX耐藥程度有相關性。 2手術治療 隨着化...
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複方肝必治治療慢性乙型肝炎高球蛋白血癥110例臨牀觀察
...反相血凝法)、抗-HBc、抗-HBe(酶聯法)、E-玫瑰花結(E-RFC)、IgG、IgM和IgA。1.4療效判斷顯效:主要症狀消失,肝、脾、腫大相應回縮,血清總膽紅素(TBil)、白蛋白(A)、球蛋白(G)、谷丙轉氨酶(ALT)恢復至正常範圍。有...
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He-Ne激光治療慢性單純性鼻炎的機制研究
...血紅蛋白、血小板、嗜酸性粒細胞、IgG、IgA、IgM、C3、A-RFc、T-RFc和LCT。1.2.3腦血流圖測量每例照射一療程前後的腦血流圖。 1.2.4抑菌試驗從50例實驗對象中任選10例作鼻腔分泌物培養,獲金黃色葡萄球菌、溶血性鏈球菌和大腸...
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武漢大學生命科學學院細胞分裂新發現登國際刊物
...國家重點實驗室的研究人員發表了題爲“ReplicationfactorC1(RFC1)isrequiredfordouble-strandbreakrepairduringmeiotichomologousrecombinationinArabidopsis”的文章,發現了一種基因:AtRFC1能通過影響同源染色體和姐妹染色單體的均等分離,調控花粉遺傳物...
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Thesinglenucleotidepolymorphism(80GA)ofreducedfolatecarriergeneintrisomy
...e;hautJeromeLejeuneInstituteParisFranceDearSir:Thereducedfolatecarriergene(RFC1orSLC19A1)locatedonchromosome21(21q22.3)codesforthereducedfolatecarrier,whichisresponsiblefor5-methyltetyrahydrofolateinternalizationwithincells.Wepublishedearlierthe80GAsingle-nucleotidepolymorphism(SNP)inhumanreducedfol...
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Effectoffolateoversupplementationonfolateuptakebyhumanintestinalandrenalepithelialcells
...gnificantdecreaseintheproteinandmRNAlevelsofthehumanreduced-folatecarrier(hRFC)andadecreaseintheactivityofthehRFCpromoter.ItwasalsoassociatedwithadecreaseinmRNAlevelsoftheproton-coupledfolatetransporter/hemecarrierprotein1(PCFT/HCP1)andfolatereceptor(FR).Conclusions:Long-termoversupplementationwithf...
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免試劑離子色譜測定陽離子中的離子抑制器應用研究
...部分2.1儀器及試劑儀器:美國DioneXICS90離子色譜儀,DionexRFC淋洗液發生器,Chromeleon色譜工作站,DS-5型電導檢測器,CSRS-I(4mm)抑制器,色譜柱爲IonPacCG16保護柱(3mm×50mm)。2.2實驗條件淋洗液:採用RFC產生的高純甲基磺酸溶液(18mmo...
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小兒反覆呼吸道感染髮病機制及最新防治
...兒童的外周血T淋巴細胞轉化率(LTT)以及活性E花環(Ea·RFC)的變化,實驗結果發現復感兒組LTT爲(59.74±7.2)%,Ea·RFC爲(21.71±4.12)%,明顯低於健康對照組,說明覆感兒T淋巴細胞數目減少,輔助細胞免疫和體液免疫...
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Diagnosisofherpessimplexvirus-1keratitisusingGiemsastain,immunofluorescenceassay,andpolymerasechainreactionassayoncornealscrapings
...mark).Theresultswereassessedunderareflectedlightfluorescencemicroscope(BH2-RFC,Olympus)withcubeBhavingfiltercombinationsforexcitationspectrumregionnear490nmforFITCstain.PositivestainingforHSVwasrepresentedbythepresenceofoneormorebasalepithelialcellsexhibitingspecificbrightapplegreenfluorescence.Apre...
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無創正壓通氣治療AECOPD併發Ⅱ型呼吸衰竭的療效觀察
...[4]。 AECOPD表現爲高氣道阻力、高VD/VT、高功能殘氣量(RFC)和高呼吸功耗等特點,高氣道阻力和內源性呼氣末正壓(PEEPi)的形成造成呼吸機疲勞,呼吸功耗增加[5]。AECOPD合併Ⅱ型呼吸衰竭使病情複雜,增加了治療難度和死亡率。...
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CRPornotCRP?ThatIstheQuestion
...geMedicalSchool,RowlandHillStreet,LondonNW32PF,UnitedKingdom.E-mailm.pepys@rfc.ucl.ac.ukC-reactiveprotein(CRP)andcardiovasculardiseaseisaveryhottopicatpresent.Excitementandinteresthavespilledoverdramaticallyfromthescientificliteratureintothemediaandpopularpressleadingtomuchspeculativecomment.However...
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Sodium-dependentmethotrexatecarrier-1isexpressedinratkidney:cloningandfunctionalcharacterization
...gorganicaniontransporter(OAT)kidney1/2(OAT-K1/OAT-K2),reducedfolatecarrier(rfc),theABCpumpmrp2,andthesodium-dependentratliverMtxtransporter-1(RL-MTX-1).Sekineetal.(34)andSweetetal.(39)independentlyclonedthefirstmemberofthesodium-independentOATfamily(OAT1)fromratkidney.ItsmRNAistranslatedintoaprotein...
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虛證
...黨蔘、白朮等藥,能提高免疫機能,若細胞免疫(E—RFC、LTT)低下時,用之更具意義。益氣解毒湯的原方設計,用於各型各期單純皰疹性角膜炎的治療,系屬辨病論治。臨牀上,當刺激證狀重者,邪盛之時,應以驅邪爲主,...
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Folateandhumanreproduction
...ynthase(A2756G),methioninesynthasereductase(A66G),andreduced-folatecarrier(RFC1;A80G)inadultswithDownsyndromeandfoundthatonlythedistributionofthevariantintheRFCgenewasdifferentfromthatofcontrolsubjects.Becauseofapossibleinfluenceoffolateinadequacyongeneticexpression,theeffectoffolicacidfortification...
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ThekidneyinvitaminB12andfolatehomeostasis:characterizationofreceptorsfortubularuptakeofvitaminsandcarrierproteins
...rbedmostlikelybyacarrier-mediatedmechanisminvolvingthereducedfolatecarrier(RFC)(86).Thedominantfolateforminserumis5-methyl-tetrahydrofolate(5-MTHF)presenteitherfree,boundtohigh-affinityfolate-bindingprotein(FBP),orlooselyassociatedwithotherserumproteinsincludingalbumin(61,132).Thefractionoffolatebou...
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Advancedglycationendproductsandthekidney
...pelGB,CartwrightK,FoilesPG,FreedmanBI,RaskinP,RatnerRE,SpiniowitzBS,WhittierFC,andWuerthJPfortheACTIONIInvestigatorGroup.Randomizedtrialofaninhibitorofformationofadvancedglycationendproductsindiabeticnephropathy.AmJNephrol24:3240,2004.BoumaB,Kroon-BatenburgLMJ,WuYP,BrünjesB,PosthumaG,KranenburgO,de...
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Rosiglitazonepromotesdevelopmentofanoveladipocytepopulationfrombonemarrow–derivedcirculatingprogenitorcells
...onjugatedisotype-matchedcontrolantibodies.CellswereanalyzedonaBeckmanCoulterFC500flowcytometer.PloidyanalysiswasperformedonadipocytessuspendedinPBScontainingsaponin(0.3%finalconcentration),PI(25μg/ml),andEDTA(0.1mM).CellswereanalyzedbyflowcytometrywithsingletdiscriminationtodetectGFP+andPI-stained,...
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RoleofCaspasesinDeathandSurvivalofthePlaqueMacrophage
...ofDNase,substratesimplicatedincelldeath,includingDFF45andreplicationfactor,RFC140,remainedintactintheseproliferatingTcellsdespitethepresenceofactivecaspases.Caspase-SensitiveKinases:ANewSignalingParadigmSurprisingly,ifcaspasesareconsideredonlyasexecutioners,manykinasesareactivatedbycaspases.Inatleas...