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第二節 無芽胞厭氧菌
...蘋果酸鹽脫氫酶、穀氨酸脫氫酶、葡萄糖6磷酸脫氫酶(G6PDH)、6磷酸葡萄糖脫氫酶(6PGDH)、細胞壁粘肽層含有二氨基庚二酸。依據對鼠李糖、海澡糖,甘露醇發酵及吲哚試驗可區別本組菌落。DNA的G+C含量爲39~48%。本菌主要分...
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Renaloxidativevulnerabilityduetochangesinmitochondrial-glutathioneandenergyhomeostasisinaratmodelofcalciumoxalateurolithiasis
...(GPx)andglutathionereductase(GR),whereasglucose-6-phosphatedehydrogenase(G6PDH)suppliesreducingequivalentsintheformofNADPH,akeycofactorinmaintainingglutathioneinitsreducedform.OxalateexposureofrenalcellsresultsinpathologicalchangesinvolvingreinitiationofDNAsynthesis,cellgrowth,cellinjury,anddeath(16...
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Survivin基因在大腸癌中的表達
...mRNA提取和逆轉錄效應的可信性,以3磷酸甘油醛脫氫酶(G3PDH)表達作爲內參照,其PCR條件與下述所描述的條件一致。Survivin基因引物序列:正向引物5’-GGAC-CACCGCATCTCTACAT-3’(位於外顯子-1的2855-2984);反向引物5’-GCACTTTGCCAGTTTCC...
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大鼠肝微粒體溫孵體系中
...泰生化試劑公司,G-6-P(6-磷酸葡萄糖),G-6-Pdehydrogenase(G-6-PDH,G-6-P脫氫酶)爲Sigma公司產品,苯巴比妥鈉(PB,藥用,進口分裝),乙腈爲色譜純,其他化學品均爲市售分析純,水爲蒸餾水經MilliQLabo純水器處理。(250±10)g雄性純種Wistar大...
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第二節 氯黴素
...酶原時間,以防藥效及毒性增強。3.對肝腎功能不良,G-6PDH缺陷者、嬰兒、孕婦、乳婦應慎用。4.用藥時間不宜過長,一般不超過二個月,能達到防止感染復發即可,避免重複療程。 製劑與用法 鹽酸四環素(tetracycline hy...
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ReducedmitochondrialdensityandincreasedIRS-1serinephosphorylationinmuscleofinsulin-resistantoffspringoftype2diabeticparents
...hondrialgenome,wasdecreasedby50%inIRoffspring(P0.05).Pyruvatedehydrogenase(PDH)andsuccinatedehydrogenase(SDH),whicharebothencodedbythenucleargenome,tendedtobedecreasedinIRoffspring(Figure3B).Figure3Mitochondrialdensityandgeneexpressiondata.(A)Mitochondrialdensityincontrolsubjects(n=6)andIRoffspring(...
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第二節 抗麻風病藥
...良反應】較常見爲貧血,偶可引起急性溶血性貧血,G-6-PDH缺乏者尤易發生。有時出現胃腸刺激症狀、頭痛、失眠、中毒性精神病及過敏反應。劑量過大還可引起肝損害及剝脫性皮炎。治療早期或增量過快,患者可發生麻風症狀...
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阿替洛爾的手性衍生化RP-HPLC法拆分及在鼠肝微粒體中的測定
...(NADPH)、GITC、6-磷酸葡萄糖(G-6-P)、6-磷酸葡萄糖脫氫酶(G-6-PDH)均購自美國Sigma公司,阿替洛爾購自中國藥品生物製品檢定所。 實驗中所用試劑均爲分析純試劑。 NaH2PO4緩衝液:精密稱取NaH2PO4.2H2O0.7801g,精確加入水500.0mL,混...
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腫瘤壞死因子α、氧化型低密度脂蛋白對人單核細胞株U937膽固醇酰基轉移酶mRNA表達的影響
...共25個循環後75℃延伸10min。同時取2μlcDNA-RNA模板,加入G3PDH引物(終濃度爲1μmol/L):5′-TCCCTCAAGATTCTCAGCAA-3′,5′-AGATCCACAACGGATACATT-3′。PCR條件爲:94℃變性1min,56℃退火1min,72℃延伸1min,共35個循環。最後72℃延伸7min。擴增後進行3...
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神經營養因子家族及其受體在重組人骨形態發生蛋白2誘導成骨中的表達
...kara一步法RTPCR試劑盒擴增小鼠NGF、BDNF、NT3及內參照G3PDH的cDNA。經15%瓊脂糖凝膠電泳後,採用凝膠圖像系統進行圖像採集和分析。根據小鼠上述cDNA序列設計引物:NGF上游引物:5′ATAAAGGTTTTGCCAAGGACG3′,下游引物:5′...
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Hypoxia-inducedneutrophilsurvivalismediatedbyHIF-1–dependentNF-Bactivity
...ver,withamoreprolongedculture(6h),glyceraldehyde3-phosphatedehydrogenase(G3PDH),MIF,triosephosphateisomerse-1,andNF-B(p65)showedatwofoldormorerelativeincreaseinexpressioninbothhypoxiaandanoxiacomparedwithnormoxia(Fig.6A).ThiswasshowntobesignificantbyBayesianttests(Fig.6A,table).Wesubsequentlyconfirm...
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地蒽酚療法的進展
...活性物質[11],它能在短期內抑制6-磷酸葡萄糖脫氫酶(G-6PDH)的活性,通過照射紫外線,能促進這種高生物活性物質的形成。同時,日光、分子氧、焦油、鹼溶液等也會促進地蒽酚氧化。因此,經典的聯合療法就是加照UVB,或加...
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Reperfusion-InducedTranslocationofPKCtoCardiacMitochondriaPreventsPyruvateDehydrogenaseReactivation
...sociatedwithlossintheactivityofthemitochondrialenzymepyruvatedehydrogenase(PDH).PharmacologicalstimulationofPDHactivityimprovesrecoveryincontractilefunctionduringreperfusion.SignalingmechanismsthatcontrolinhibitionandreactivationofPDHduringreperfusionwerethereforeinvestigated.Usinganisolatedratheart...
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毛細血管血血糖和靜脈血漿血糖相關性分析
...肘靜脈血,用奧林巴斯AU640生化分析儀,己糖激酶(G6PDH)方法測定空腹肘靜脈血漿血糖。 1.3質量控制方法由專人負責血樣的離心、凍存及血糖檢測,爲了控制質量,課題組按5%比例進行抽樣複查,結果的一致率爲95%。1...
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摩托羅拉“藥丸”的背後:身體密碼讓人歡喜讓人憂
...管理局(FDA)就曾通過了一款由普羅透斯數字健康協會(PDH)發明的數字藥丸,以確保患者及時使用他們的藥丸。 PDH開發的硅芯片,小如一粒細沙。人體通過與消化液互動,可激發藥丸內的銅和鎂而產生電壓,以激發其發揮...
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小瞼裂綜合徵FOXL2基因突變分析
...放性閱讀框及5’非翻譯區,產物直接或克隆後測序。用PDH和ExPASy軟件包對正常及發生突變後的蛋白質結構進行預測,分析比較它們一、二級結構的差異。對小瞼裂綜合徵(BPES)患者進行FOXL2基因突變篩選,並對基因突變前後的...
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ThromboxaneA2PromotesInterleukin-6BiosynthesisMediatedbyanActivationofCyclicAMP-ResponseElement-BindingProteinin1321N1HumanAstrocytomaCells
...or30s,57°Cfor60s,and72°Cfor60s).Glyceraldehyde3-phosphatedehydrogenase(G3PDH)transcriptsof700basepairswereusedasaninternalcontrol.Thenumberofcyclesthatyieldedaquantitativeamountofproductwasdeterminedinpreliminaryexperiments.ThedensitiesofthebandscorrespondingtoIL-6andG3PDHwereanalyzedbydensitometr...
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第二節 糖的分解代謝
...在脊椎動物還有第二層次的調節,即酶蛋白的化學修飾,PDH含有兩個亞基,其中一個亞基上特定的一個絲氨酸殘基經磷酸化後,酶活性就受抑制,脫磷酸化活性就恢復,磷酸化-脫磷酸化作用是由特異的磷酸激酶和磷酸蛋白磷酸...
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Emerin-LackingMiceShowMinimalMotorandCardiacDysfunctionswithNuclear-AssociatedVacuoles
...TGATGGAAGGTATCAGCATCTACA-3‘)andglyceraldehyde-3-phosphatedehydrogenase(G3PDH)(forward:5‘-CTGGAGAAACCTGCCAAGTATG-3‘,reverse:5‘-TTGAAGTCGCAGGAGACAACCTG-3‘)wereused.ThevaluesofmRNAforemerinwerenormalizedtothatofG3PDH.ClinicalExaminationsEachmousewasweighedevery2weeksfrom3weeksofage.Musclestre...
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流感加重或源於特定激酶“搗亂”有望研發防治新藥
...著增多。PDK4會使實驗鼠體內與能量生產相關的另一種酶PDH的作用減弱。其結果是細胞內能量枯竭,抵抗力下降,進而導致肺炎、多臟器衰竭等。研究人員對患重症流感的40只實驗鼠使用可抑制PDK4作用的藥物,這些實驗鼠兩週後...
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IncreasedCollagenDepositionandDiastolicDysfunctionbutPreservedMyocardialHypertrophyAfterPressureOverloadinMiceLackingPKC
...):5‘-ATCTCACGTTCAGCTGTGGTCA-3‘;5‘-ACCACCGGCATCG-TGTTGGAT-3‘;22andG3PDH:5‘-AACGACCCCTTCATTGAC-3;5‘-TCCACGACATACTCAGCAC-3.PCRwasperformedwithdenaturationat95°Cfor1minute,annealingat57°Cfor1minute,andelongationat72°Cfor30seconds(40cycles).Dataacquisitionwasaccomplishedat80°Ctoavoidmeasu...
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Ornithinemetabolisminmaleandfemaleratkidney:mitochondrialexpressionofornithineaminotransferaseandarginaseII
...1:2,000);andamonoclonalmouseanti-glyceraldehyde-3-phosphatedehydrogenase(G3PDH;dilution1:170).Theblotswerewashedthreetimesfor20minin1xTBSTandincubatedfor60minwitheitherperoxidase-conjugatedanti-rabbitIgGoranti-mouseIgGsecondaryantibodies(dilution1:10,000)in5%milk-1xTBST.Blotswerewashedthreetimesfor1...
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PolyunsaturatedfattyacidssuppressglycolyticandlipogenicgenesthroughtheinhibitionofChREBPnuclearproteintranslocation
...ate-limitingenzymeofglycolysis)andofglucose6-phosphate(G6P)dehydrogenase(G6PDH;therate-limitingenzymeofthepentosephosphatepathway)requiredforthesynthesisofX5PfromG6P.RefeedingmicewithaHCHOdietfor18hoursorculturinghepatocytesinthepresenceof25mMglucoseand100nMinsulinfor24hoursledtoaparallelincreaseinG...
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DNAMethyltransferaseInhibitorsCoordinatelyInduceExpressionoftheHumanReelinandGlutamicAcidDecarboxylase67Genes
...isolatedafterultracentrifugationthroughCsCl(Chenetal.,2002).Reelin,GAD67,G3PDH,andDNMT1mRNAcontentsweremeasuredbycompetitiveRT-PCRwithinternalstandardsasdescribedpreviously(GraysonandIkonomovic,1999).Primersweredesignedtominimallycrossatleastoneexon/intronboundary.Forexample,the5‘DNMT1primerreside...
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特定基因或蛋白質的數據庫
...ontainingsystems IARCp53db-IARCdbofsomaticp53mutations PDZdomain-PDH/DHRdomainWWWpage PDE-Phosphodiesterases Peroxidases PlantPeroxidases PeptidasesinSWISS-PROT-NomenclatureandindexofpeptidasesinSWISS-PROT MEROPS-Peptidasedb Prolysis-Proteasesandinhibitorsserv...
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高中生聽力損失1924例流行病學調查
...5、1、2、3、4、6、8kHz7個頻率[1]。 1.4診斷標準根據WHO/PDH97.3推薦標準,以0.25之4kHz平均聽閾對受試者進行聽力損失程度的分級:輕度26~40dBHL,中度41~60dBHL,重度61~80dBHL,極重度≥81dBHL[2]。 1.5統計學方法用SPSS13.0軟件進...
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MitochondrialNADP+-DependentIsocitrateDehydrogenaseProtectsCadmium-InducedApoptosis
...eNADPHpoolandGSHrecycling(Leeetal.,2003).Glucose6-phosphatedehydrogenase(G6PDH)isimportantintheproductionofNADPHforGSHrecyclingincytosol.IthasbeenshownthatalowerconcentrationofCd2+treatmentincreasestheexpressionofG6PDHasmeasuredbymRNAlevelsandenzymeactivityinrathepatocytes(Xuetal.,2003).Furthermore,...
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Inflammationandthereciprocalproductionofgranulocytesandlymphocytesinbonemarrow
...cientinCXCL12.mRNAspecificforBcell–activatingfactor(BAFF),CXCL2,CXCL12,GAPDH,GM-CSF,hypoxanthine-guaninephosphoribosyltransferase(HPRT),IL-1,IL-3,IL-7,andSCFwerequantifiedrelativetoGAPDHmessageatvarioustimesafterimmunization(TableIandFig.3A).Asexpected,CXCL12messagedecreased10-foldatdays4and8after...
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HypochlorousAcid,aMacrophageProduct,InducesEndothelialApoptosisandTissueFactorExpression
...AAGCAGTGA-TTCCCTCTCG-3‘;antisense,5‘-AACACAGCATTGGCAGCAG-3‘andhumanG3PDH:sense,5‘-GGAGCCAAA-AGGGTCATC-3‘;antisense,5‘-CCAGTGAGTTTCCCGTTC-3‘.WeconductedPCRfor25cycles(confirmedtobewithinthelog-linearrangeofamplification)forTFandG3PDH,andtheproductswereelectrophoresedthrougha2.0%agarose-...
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綠激光前列腺汽化(PVP)治療前列腺癌導致的下尿路梗阻降低前列腺癌播散危險性的臨牀研究
...12]。這種設計保證了PSA反轉錄PCR的產品是來自PSA的mRNA。GAPDH的引物序列爲:順向引物CGACCACTTTGTCAAGCTCA,逆向引物AGGGGAGATTCAGTGTGGTG。④PCR反應條件:PCR反應用了5μL的cDNA。反應總量爲25μL,包括2.5μLPCR緩衝液(Invitrogen,英國),0.75μLMgCl2...