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HostProstaglandinE2-EP3SignalingRegulatesTumor-AssociatedAngiogenesisandTumorGrowth
...s.*P0.05versusWTanimals(Student‘sttest).AllexperimentswereperformedusingmaleC57BL/6micewithandwithoutdisruptionofEPreceptorsubtypesorIPreceptor. SpongeImplantationModelofAngiogenesis.Spongedisks(thickness,5mm;diameter,1.3cm;references7and8)wereimplantedunderlightetheranesthesiaintothesubcutaneou...
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Dietaryconjugatedlinoleicacidandbodycomposition
...CLA,whichcontained34%t10,c12-isomerand33%c9,t11-isomer,reducedweightgaininmaleC57BL/6JmiceandICRmice(30).SimilarresultswerereportedinZuckerDiabeticFatty(ZDF)rats(20)andob/obmice(31).InweanlingfemaleICRmice,feeding0.250.50%CLA,containing79%ofthet10,c12-isomer,showedloweredbodyweights.Nobodyweig...
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Darbepoetin-AlphaDoesNotPromoteMicrovascularThrombusFormationinMice
...ytosisincreasetheriskofthrombusformation.MethodsAnimalsandMaterialsAnimalsMaleC57BL/6JmiceandeNOS(-/-)mice(B6.129P2/Nos3,bredonC57BL/6Jbackground)werepurchasedfromCharlesRiverLaboratories,Sulzfeld,Germany.ChemicalsDarbepoetin-alpha(DPO)waspurchasedfromAmgen(AmgenInc)anddissolvedinphysiologicalsaline...
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HepatocyteGrowthFactorRegulatesEBox-DependentPlasminogenActivatorInhibitorType1GeneExpressioninHepG2LiverCells
...nesofHokkaidoUniversityandwereapprovedbyInstitutionalAnimalStudycommittee.MaleC57BL/6Jmice(CLEAJapan,Tokyo)hadfreeaccesstochowandwater.At8weeks(20to22g)miceweregivenHGForsaline.After4hoursanesthesiawasinducedwithether.Liverswereexcised,rinsedwithPBSandsnap-frozeninliquidnitrogenforRNAextraction.West...
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MicroglialRecruitmentActivationandProliferationinResponsetoPrimaryDemyelination
...cingthemicebackonnormalfoodfor3weeks.Unlessotherwisespecified,8-week-oldfemaleC57BL/6mice,purchasedfromTheJacksonLaboratory(BarHarbor,ME),wereusedintheseexperiments.ToinvestigateeffectsofCCL2-CCR2signaling,CCL2-deficientmice(10generationsC57BL/6;TheJacksonLaboratory25)andCCR2-deficientmice(F2C57BL/6...