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CriticalfunctionofBmx/Etkinischemia-mediatedarteriogenesisandangiogenesis
...constitutivelyactiveformofBmxundertheendothelialTie-2enhancer/promoter(Bmx-SK-Tgmice)tostudytheroleofBmxinischemia-mediatedarteriogenesis/angiogenesis.Inresponsetoischemia,Bmx-KOmicehadmarkedlyreduced,whereasBmx-SK-Tgmicehadenhanced,clinicalrecovery,limbperfusion,andischemicreservecapacitywhencompar...
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UseofPhylogeneticandPhenotypicAnalysesToIdentifyNonhemolyticStreptococciIsolatedfromBacteremicPatients
...rposes,24typeand9referencestrainswereanalyzed:S.sanguinisstrainsATCC10556T/SK1,SK4,andSK36(S.sanguinisstrainwhosegenomeiscurrentlybeingsequencedatVirginiaCommonwealthUniversity;www.sanguinis.mic.vcu.edu);S.oralisstrainsNCTC11427T/SK23andSK34;S.gordoniistrainsATCC10558T/SK3andChallis/SK7;S.mitisstrai...
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RenalexpressionandactivityofthegerminalcenterkinaseSK
...inkinases.ThisscreeningdemonstratedexpressionofaproteinkinaseconsistentwithSK2,agroupIIgerminalcenterkinaseandhomologofhumanSte20-likekinase(SLK).SK2mRNA,proteinexpression,andkinaseactivitywereincreasedinratfetalkidneyhomogenates(embryonicdays17-21)comparedwithadultcontrols.Inadultkidneyssubjectedto...
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載脂蛋白E在神經細胞中表達對細胞功能的影響
...時,探討apoE4在AD發病中的相關機制。研究方法篩選轉染SK-N-SH定位觀察表達鑑定功能分析構建真核表達質粒重組pEGFP-apoE質粒酶切電泳圖apoE3Query:321cgcaggcccggctgggcgcggacatggaggacgtgtgcggccgcctggtgcagtaccgcg380||||||||||||||||||||||||||||||||||||||||||||||...
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CentralEuropeanDobravaHantavirusIsolatefromaStripedFieldMouse(Apodemusagrarius)
InstituteofVirology,HelmutRuskaHaus,ChariteSchoolofMedicine,HumboldtUniversity,D-10098Berlin,GermanyInstituteofVirologyInstituteofZoology,SlovakAcademyofSciences,84206Bratislava,SlovakiaABSTRACTDobravavirus(DOBV)isahantavirusthatcauseshemorrhagicfeverwithrenalsyndrome(HFRS)inEurope.Itishostedbyatlea...
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InhibitoryGatingModulationofSmallConductanceCa2+-ActivatedK+ChannelsbytheSyntheticCompound(R)-N-(Benzimidazol-2-yl)-1,2,3,4-tetrahydro-1-naphtyla
【關鍵詞】InhibitoryGatingModulationSKchannelsaresmallconductanceCa2+-activatedK+channelsimportantforthecontrolofneuronalexcitability,thefinetuningoffiringpatterns,andtheregulationofsynapticmechanisms.TheclassicSKchannelpharmacologyhaslargelyfocusedonthepeptideapamin,whichactsextracellularlybya...
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Bmi-IsEssentialfortheTumorigenicityofNeuroblastomaCells
...eeoftheMedicalUniversityofOhio.CellCultureHumanneuroblastomacelllinesSHEP1,SK-N-AS,SK-N-F1,SK-N-DZ,andSK-N-SHweregrowninDulbecco??smodifiedEagle??smediumsupplementedwith10%fetalbovineserum(FBS);IMR32,SK-N-BE(2),BE(2)-C,SY5Y,andLA1C55Nina1:1mixtureofDulbecco??smodifiedEagle??smediumandHam??snutrientm...
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Incidenceofkeratitisofvaryingseverityamongcontactlenswearers
...ation29August2004ABSTRACTAim:Todeterminetheincidenceofnon-severekeratitis(NSK)andseverekeratitis(SK)amongwearersofcurrentgenerationcontactlenses.Methods:A12month,prospective,hospitalbasedepidemiologicalstudywasconductedbyexaminingallcontactlenswearerspresentingwithacornealinfiltrate/ulcertoahospital...
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ReceptorRegulationofGeneExpressionofAxonGuidanceMolecules:ImplicationsforAdaptation
...s)andmodulationofaxonguidancemolecules,weinvestigatedwhetherGPCRactivityinaSK-N-MChumanneuroepitheliomacellline(whichexpresseslowlevelsofD1dopaminereceptors)affectsgeneexpressionofaxonguidancemolecules(semaphorins,ephrins,netrins,andtheirreceptors).Usingreal-timepolymerasechainreaction,weidentified1...
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小劑量重組織型纖溶酶原激活劑與尿激酶、重組鏈激酶溶栓治療急性心肌梗死的臨牀對比分析
...纖溶酶原激活劑(rt-PA)、尿激酶(UK)和重組鏈激酶(r-SK)治療急性心肌梗死的療效和安全性。方法114例急性心肌梗死患者隨機分爲rt-PA組38例,UK組37例,r-SK組39例。分別應用纖溶酶原激活劑50mg、尿激酶150萬u、鏈激酶150萬u靜...
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EndothelialNitricOxideSynthaseActivationbyTumorNecrosisFactorThroughNeutralSphingomyelinase2,SphingosineKinase1,andSphingosine1PhosphateRe
...ialactivationofneutral-sphingomyelinase-2(N-SMase2)andsphingosine-kinase-1(SK1)andgenerationofsphingosine-1-phosphate(Sph1P).InhibitionofN-SMase2inhibitedSph1Pformation,whereasinhibitionofSK1didnotaffectN-SMase2activationbyTNF-.BlockadeofN-SMase2,SK1,ortheSph1PreceptorsS1P1andS1P3,eitherbysilencingo...
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Depressionandcardiovascularmortality:aroleforn3fattyacids?
...sandPrimaryCare,UniversityMedicalCenterUtrecht,Utrecht,Netherlands(MHK,MIG,SK,andDEG);theCentreforPreventionandHealthServicesResearch,NationalInstituteforPublicHealthandtheEnvironment,Bilthoven,Netherlands(MART);andtheDivisionofHumanNutrition,WageningenUniversity,Wageningen,Netherlands(DK)2MarjaARTi...
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EvidenceforInvolvementofBothIKCaandSKCaChannelsinHyperpolarizingResponsesoftheRatMiddleCerebralArtery
...ngIKCachannelsalone,contrastingwithperipheralvesselswhereblockofbothIKCaandSKCaisrequired.AsthecontributionofIKCaandSKCatoendothelium-dependenthyperpolarizationdiffersinperipheralarteries,dependingonthelevelofarterialconstriction,weinvestigatedthepossibilitythatSKCamightcontributetoequivalenthyperpo...
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NeuroprotectiveEffectsof17-EstradiolandNonfeminizingEstrogensagainstH2O2ToxicityinHumanNeuroblastomaSK-N-SHCells
...tiveagents.EpidemiologicalstudiesshowthatearlyestrogentherapycanreducetheriskofneurodegenerativediseasessuchasAlzheimer‘sdiseaseandimprovecognitionandmemoryinADpatients(Hendersonetal.,1994;Birge,1996;Sherwin,1996;Tangetal.,1996;Yaffeetal.,1998;Costaetal.,1999;Baggeretal.,2005).Inaddition,estrogent...
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TheH+-LinkedMonocarboxylateTransporter(MCT1/SLC16A1):APotentialTherapeuticTargetforHigh-RiskNeuroblastoma
...T1mRNAlevelsinfreshneuroblastomabiopsysamplesthatcorrelatedpositivelywithriskoffataldiseaseandamplificationofthe“proto-oncogenic“transcriptionfactorMYCN.WefurtherinvestigatedMCTasapotentialtherapeutictargetinvitro.TheneuroblastomacelllinesevaluatedwereSk-N-SH,CHP134,IMR32,andNGP.Alllinesexhibite...
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IronChelationbyClinicallyRelevantAnthracyclines:AlterationinExpressionofIron-RegulatedGenesandAtypicalChangesinIntracellularIronDistribution
...ithDOXcouldnotprotectcellsfromthetoxicityofsubsequentironloadingbyFAC.HumanSK-Mel-28melanomacellsorratH9c2cardiomyocyteswerepreincubatedwithCONor5µMDOXfor24hat37°C,andthentheywerewashed.Thecellswerethenreincubatedfor16hat37°CwithCONcontainingincreasingconcentrationsofFAC(250,500,750,an...
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脫水蛋白在逆境下的分子作用機制研究進展
...結構保守性強,具有K,S和Y等3個高度保守區域,通常以YSK的順序結合在一起,中間夾有低保守性、高重複性的φ片段。Y片段爲(V/T)DEYGNP,位於N末端,通常有1—3個串連拷貝,推測與植物和細菌中蛋白的核定位有關口;K片段(EKKGIMD...
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ROMKisrequiredforexpressionofthe70-pSKchannelinthethickascendinglimb
...ceexhibitaBartterphenotypeandlackthesmall-conductance(30-pS)apicalKchannel(SK)intheTAL.However,adistinct70-pSKchannelcanalsosignificantlycontributetotheapicalconductanceofTAL.WenowexaminetheeffectofROMKdeletiononthefunctionalexpressionofthe70-pSKchannelintheTAL.Functionalexpressionofthe70-pSKchannel...
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膠質瘤患者樹突狀細胞在裸鼠體內外誘導抗腫瘤免疫
...來自遼寧省錦州市中心醫院臨牀確診病人;膠質瘤細胞株SK-MG-1購自北京醫科大學;SGC-7901人胃癌細胞株、BeL-7402人肝癌細胞株購自中山大學。4周齡BALB/C裸鼠購自北京醫科大學。1.2方法1.2.1製備凍融抗原取107個SK-MG-1膠質瘤細胞,加...
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老年急性心肌梗死溶栓治療對左心室功能的改善作用
...[1,2],對老年急性心肌梗死(AMI)患者應用鏈激酶(SK)溶栓治療,能明顯降低AMI的病死率,但其對心功能的影響研究尚少。我們研究了2002年2月~2003年6月初次AMI接受SK靜脈溶栓治療的老年患者29例,探討溶栓再灌注對左心室...
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患肢動脈內注射r-SK對下肢深靜脈血栓形成的效果
[摘要]目的探討患肢動脈注射重組鏈激酶(r-SK)治療下肢深靜脈血栓形成的安全性和有效性。方法對31例下肢深靜脈血栓形成病人,從健側股動脈穿刺插管至患側髂股動脈內。r-SK經下肢動脈注入,並動脈內保留導管,每日溶...
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SK能源涉足海藻類生物能開發
近日,SK能源宣佈即將與釜山釜慶大學簽訂協議,正式涉足海藻類生物能的開發。 通過本次協議,SK能源與釜慶大學決定爲確保海藻類生物的數量進行相關的技術開發和合作研究。SK能源相關者表示,釜慶大學擁有海藻類的相...
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Phosphorylation-dependenttranslocationofsphingosinekinasetotheplasmamembranedrivesitsoncogenicsignalling
...tofMedicine,UniversityofAdelaide,AdelaideSA5000,AustraliaSphingosinekinase(SK)1catalyzestheformationofthebioactivelipidsphingosine1-phosphate,andhasbeenimplicatedinseveralbiologicalprocessesinmammaliancells,includingenhancedproliferation,inhibitionofapoptosis,andoncogenesis.HumanSK(hSK)1possesseshig...
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SensitivityandspecificityofscanninglaserpolarimetryusingtheGDx
...s.TheGDxprintoutsofallsubjectswereevaluatedbythreeindependentobserversinamaskedfashionandwithouttheclinicalpictureoftheopticdisc.Twoofthethreeobservers(SK,UH)wereGDxexperts,one(KUL)wasanuntrainedGDxuser.Results:AmongtheGDxexperts,sensitivity/specificitywas100%/100%(SK)and90%/100%(UH)indetectingadvan...
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第三節 纖維蛋白溶解藥
...藥的工作正在進行。 鏈激酶 鏈激酶(streptokinase,SK)是C組β溶血性鏈球菌產生的一種蛋白質,能與纖溶酶原結合,形成SK-纖溶酶原複合物後,促使遊離的纖溶酶原轉變成纖溶酶,溶解纖維蛋白。因此,需選合適劑量以發...
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急性心肌梗死溶栓藥物的研發和臨牀評價
...劑量可能爲150萬IU左右,尿激酶治療AMI有效[3]。2鏈激酶(SK)鏈激酶是一種蛋白質,由C組b溶血性鏈球菌的培養液提純精製而得,分子量爲47kD,血漿半衰期18-33分鐘,血池衰減率每小時20%。SK不直接激活纖溶酶原,而是通過與纖溶...
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國產重組鏈激酶治療急性心肌梗死臨牀分析
...在基層醫院。常用的溶栓藥物有尿激酶(UK)、鏈激酶(SK)和組織型纖溶酶原激活劑(rt-PA),但各自的價格和再通率不完全相同,選擇一種價位適中,再通率高的溶栓劑尤其重要,本文將對國產尿激酶(UK)及國產重組鏈激酶...
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SK計劃開發生物基丁二烯技術
韓國SK創新公司和低碳燃料生產商朗澤科技近日宣佈,雙方正合作開發生物基1,3-丁二烯的生產技術。此次合作將加快丁二烯替代生產路線的商業化進程。受美國頁岩氣革命大獲成功的影響,丁二烯正變得日益稀缺。多年來,SK在...
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重組鏈激酶治療下肢深靜脈血栓形成的療效觀察
【摘要】目的探討重組鏈激酶(r-SK)治療下肢深靜脈血栓形成的療效。方法選取2003年5月~12月湘雅醫院收治的38例深靜脈血栓形成的病人,按入院先後隨機單盲分爲兩組,試驗組(重組鏈激酶組)20例,採用重組鏈激酶50萬U(思...
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Down-RegulationofInhibitorofApoptosisProteinsbyDeguelinSelectivelyInducesApoptosisinBreastCancerCells
...breastcancerandnormalcells.CellLinesAndMaterials.HumanbreastcancercelllinesSK-BR-3andMCF-7andnormalimmortalizedhumanmammaryepithelialcelllineMCF10AwereobtainedfromtheAmericanTypeCultureCollection(Manassas,VA)andculturedinmediumaccordingtotheirrecommendations.Theprimarynormalhumandermalfibroblastcell...