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磷酸甘油酸激酶的研究進展
...510089,Guangdong,PRChina)磷酸甘油酸激酶(PhosphoglyceratekinasePGK)是糖酵解的關鍵酶,也是每種生物得以生存的必須酶,該酶的缺乏可引起生物體代謝等功能的紊亂。PGK是一個單體的、高度柔曲性的糖酵解酶,它主要由兩個球形的結...
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可溶性TNF受體在大腸桿菌中表達
...經XbalⅠ酶切、連接、轉化大腸桿菌JM101,構成新的重組子pGK-sTNFR。重組質粒經XbalⅠ,TaqI酶切鑑定及採用雙脫氧鏈終止法對sTNFR進行DNA序列分析表明,所克隆的sTNFR基因與報道的一致(見圖1)。 圖1 pGK-sTNFR...
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糖直接酵解徑路中酶缺乏引起的溶血性貧血有哪些?
...值。本病由常染色體隱性遺傳。 (8)磷酸甘油酸激酶(PGK)缺乏 PGK在1,3二磷酸甘油酸產生三磷酸腺苷(ATP)及三磷酸甘油酸產生二磷酸腺苷(ADP)的過程中均有作用,PGK缺乏時ATP的生成會明顯減少,結果影響紅細胞膜的功能。患...
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低氧環境影響小鼠未成熟關節透明軟骨細胞培養
...-1(GLUT-1)、葡萄糖轉運體-3(GLUT-3)、磷酸果糖激酶-1(PGK-1)和低氧誘導因子-1α(HIF—1α)的表達。應用定量PCR觀察軟骨細胞表型改變。應用四甲基偶氮唑鹽法(MTT)檢測低氧及氯化鈷對軟骨細胞活性的影響。用葡萄糖檢測試...
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第二軍醫大Hepatology解析非編碼RNA與癌症
...MVIH有可能通過抑制磷酸甘油酸激酶1(Phosphoglyceratekinase1,PGK1)促進了腫瘤誘導的血管生成。此外,在65個肝癌樣本中,研究人員證實MVIH的表達與PGK1血清水平負相關,與微血管密度正相關。這些研究結果表明lncRNAMVIH失控可作爲肝切...
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被癌細胞遺棄的激酶PKG
...組織(確保癌組織被全部清除)相比,PKG水平明顯不同。PGK水平的變化究竟是手術造成的還是癌細胞存活的關鍵環節?許多蛋白都被癌細胞丟失了,那麼將PGK重新放回癌細胞會有怎樣後果呢?Browning首先向轉移性結直腸癌細胞再...
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上海應物所等提出鋁對人體生物毒性的新分子機制
...白的二級結構,使蛋白產生不可逆的變性和積聚。他們用PGK蛋白爲例子,通過同步輻射能譜和XPS、CD、NMR等實驗證明了這一點:1)CD實驗顯示,鋁離子可以使PGK蛋白產生不可逆變性、積聚;2)同步輻射能譜和XPS實驗表明,蛋白積...
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TargetingthelocaltumormicroenvironmentwithvacciniavirusexpressingB7.1forthetreatmentofmelanoma
...ld(CT)valuesofgeneexpressionfortheendogenouscontrolphosphoglyceratekinase1(PGK1)werecomparedtoruleoutdifferencesinaRNAassessmentduringprocessingpriortoqRT-PCRanalysis.Therewerenodifferencesfoundamongall5prevaccination(20.08±0.98)and16postvaccination(20.1±1.2)PGK1CTvaluesfrompatients3,4,5,7,and9.Am...
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南京大學首席科學家Cell子刊揭示表觀遺傳修飾酶的新功能
...調控開關)。去年,來自復旦大學的研究人員證實HDAC3是PGK1的去乙酰化酶。胰島素及mTOR信號通路調控了HDAC3介導的PGK1去乙酰化及激活(延伸閱讀:復旦管坤良、熊躍、葉丹聯合發佈表觀遺傳新成果)。在覈心的哺乳動物晝夜節...
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TheImpactofHypoxicTreatmentontheExpressionofPhosphoglycerateKinaseandtheCytotoxicityofTroxacitabineandGemcitabine
...heamountofthetriphosphateform(L-OddCTP)innuclearDNA.Phosphoglyceratekinase(PGK),adownstreamproteinofhypoxia-inducible-factor-1(HIF-1),isresponsibleforthephosphorylationofthediphosphatetothetriphosphateofL-OddC.Inthisstudy,westudiedtheimpactofhypoxiaonthemetabolismandthecytotoxicityofL-OddCand-D-2‘...
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哪些紅細胞酶缺乏可引起貧血?
...脫氫酶(PGD)、二磷酸甘油酸變位酶(DPGM)、磷酸甘油酸激酶(PGK)、丙酮酸激酶(PK)、三磷酸腺苷酶(ATPase); ②磷酸己糖旁路(HMP)中的酶:葡糖六磷酸脫氫酶(G6PD)、六磷酸葡糖酸脫氫酶(6PGD)、谷胱甘肽過氧化物酶(GSH-Px)、谷胱甘肽還...
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田世平研究組發現轉錄因子RIN調控果實成熟衰老分子機制
...化酶類似蛋白E8)、糖酵解相關基因(磷酸甘油激酶前體PGK)、次生代謝相關基因(聚精液素醛酯酶PNAE),以及芳香物質代謝相關基因(脂氧合酶LOX和乙醇脫氫酶ADH2)的表達,RIN蛋白能夠與這些基因的啓動子序列結合。RIN基因...
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青黴素裂解液的高效液相色譜分析
...radedproductsofpenicillin,6-aminopenicillanicacid(6-APA),penicillinGkalium(PGK),phenylaceticacid(PAA),andtheby-productbenzylpenicilloicacid(BPA)byHPLCwasstudied.Separationconditionswereasfollows:SpherisorbC18column,250mm×4.6mmi.d.,10μm;mobilephase:V(methanol)∶V(0.004mol/LKH2PO4buffer,pH4.5)=50...
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青黴素裂解液的高效液相色譜分析
...radedproductsofpenicillin,6-aminopenicillanicacid(6-APA),penicillinGkalium(PGK),phenylaceticacid(PAA),andtheby-productbenzylpenicilloicacid(BPA)byHPLCwasstudied.Separationconditionswereasfollows:SpherisorbC18column,250mm×4.6mmi.d.,10μm;mobilephase:V(methanol)∶V(0.004mol/LKH2PO4buffer,pH4.5)=50...
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基因芯片篩選口腔頜面鱗癌順鉑耐藥和敏感相關基因
...和FANCONI,GRP58,FLJ12089和SPINT-2基因上調,而FOSL1,MRPS27和PGK-1基因下調(OncolRep.2005Apr;13(4):709-14.)。韓國蔚山大學爲了確定調節順鉑敏感的相關基因,採用基因芯片技術,研究了源自順鉑敏感和順鉑抵抗的細胞株的基因表達譜,...
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RegulationofTypeIICollagenSynthesisduringOsteoarthritisbyProlyl--Hydroxylases
...alHIF-1targetgenes,glucosetransporter-1(Glut-1)andphosphoglyceratekinase-1(PGK-1).【關鍵詞】regulationcollagensynthesisosteoarthritisprolyl--hydroxylasesMaterialsandMethodsPatientsandSamplesforImmunohistochemistryCartilage/bonesampleswereobtainedfrompatientswhowerehospitalizedintheDivisionofOrt...
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CriticalrolesoftheimmunoglobulinintronicenhancersinmaintainingthesequentialrearrangementofIgHandIgkloci
...r;MSRE-QPCR,methylation-sensitiverestrictionenzymereal-timequantitativePCR;PGK,phosphoglycerolkinase;RS,recombiningsequence;RSS,recombinationsignalsequence;SE,signalend.M.A.InlayandT.Lincontributedequallytothiswork.TheIgandTCRgenesareassembledthroughthesomaticrecombinationofV(Variable),D(Diversity),...
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ComprehensiveTranscriptomeofProteasesandProteaseInhibitorsinVascularCells
...ddedtoeachreaction.Forthispurpose,weused6humanhousekeepinggenes:PPIA,GAPDH,PGK1,EEF1a,RPL13A,andubiquitin.Moreover,usingGeNorm,7weselectedforeachtime2ofthemoststablehousekeepinggenesacrossallofthespecimensandusedtheirgeomeansfornormalization.NormalizeddataarepresentedinTables1,2,and3asrelativegeneco...
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ProtectionofHIF-1-deficientprimaryrenaltubularepithelialcellsfromhypoxia-inducedcelldeathisglucosedependent
...tcaacacc-3‘.PrimersequencesandreactionconditionsforHif1aexon-2,Bax,Bnip3,Pgk,and-actinwereusedasdescribedbefore(6).Chemicals.2-Deoxyglucose(2-DOG)andphloretinwerepurchasedfromSigma.Statisticalanalysis.Atwo-tailedStudent’st-testwasusedforstatisticalanalysis.Pvaluesof<0.05wereconsideredstatistical...
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BaffledbyBafilomycin:AnAnticancerAgentThatInducesHypoxia-InducibleFactor-1Expression
...aspectsofcancerbiology:VEGFandSDF1stimulatetumorangiogenesis;GLUT1,ENO1,andPGK1stimulateglucosetransportandaerobicglycolysis;EPO,IGF2,andTGFAparticipateinautocrinegrowthfactorsignaling;andmultidrugtransportersABCB1andABCG2promotechemotherapyresistance(HirotaandSemenza,2006).Inaddition,HIF-1activates...
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X染色體與腫瘤的關係研究進展
...21)、TFM(Xp11-q13)、AR(Xp11-q21)、XCE(Xp11.2-q21.1)、PGK(Xp13)、CID(Xp13-q22)、GLA(Xp21-q24)、Alport(Xp22)和HPRT(Xp26)。常用的限制性內切酶有HpaⅡ、HhaⅠ、BgⅠ等[3,4,7,11,12]。用這些方法研究證明急性非淋巴細胞...
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HyperproductionofHyaluronaninNeu-InducedMammaryTumorAcceleratesAngiogenesisthroughStromalCellRecruitment
...ingtoexpressionofHas2mRNA.White,gray,andblacktrianglesrepresentCAGpromoter,PGK-Neo,andHas2-R7primers,respectively.B:PCRanalysisofCre-mediatedgenomicDNArecombination.GenomicDNAsampleswereisolatedfromNeu-initiatedmammarytumors.PCRscreening(#1)withtheCAGpromoterandPGK-Neoprimersgavetheanticipated360-bp...
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15d-ProstaglandinJ2ProtectsBrainFromIschemia-ReperfusionInjury
...tion-defectiverecombinantadenoviral(rAd)vectorahumanphosphoglyceratekinase(PGK)promotertodriveCOX-1(Adv-COX-1),greenfluorescentprotein(Adv-GFP),orPGKalonetoserveasthecontrol(Adv-PGK).IntracerebralVentricularInfusionofAdenoviralConstructsand15d-PGJ2Theprocedurewasperformedaspreviouslydescribed.4Brief...
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低氧誘導因子-1α與腫瘤演進
...白GLUT1、GLUT3以及糖酵解酶HK1、HK3、PFKL、ALDA、ALDC、GAPDH、PGK1、PGM、ENO1、PKM、LDHA[12]。在人類乳腺癌細胞實驗中發現,HIF-1α與糖代謝活性密切相關[13]。巨噬細胞依賴於糖酵解產生的ATP而維持其功能,HIF-1α缺失的巨噬細胞ATP...
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MutantFibrinogenClearedfromtheEndoplasmicReticulumviaEndoplasmicReticulum-AssociatedProteinDegradationandAutophagy
...lotanalysisforthepresenceofthecytosolicmarkerproteinphosphoglyceratekinase(PGK),anERluminalmarkerproteinBiP,andfibrinogen.PGKwasseenonlyinthecytosolfraction,asexpected.Conversely,HMWAguadillaDalongwiththeERluminalchaperoneBiPresidedexclusivelywithinthemicrosomefraction(Figure5A).Todeterminewhetherth...
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AbsenceofbacteriallyinducedRELM?reducesinjuryinthedextransodiumsulfatemodelofcolitis
...t.(A)Schematicdiagramoftheknockoutstrategy.Thephosphoglyceratekinase–Neo(PGK-Neo)cassettewasusedtodisrupt2323bpoftheRELM?genelocus,replacingthepromoterregionandthefirst2exons.(B)GenomicPCRusingprimersshowninA.Amplificationofa295-bpproductbyprimersAandBindicatethewild-typeallele(lowerband),whereasa...
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Leisure-timeactivityisanimportantdeterminantoflong-termweightmaintenanceafterweightlossintheSibutramineTrialonObesityReductionandMaintena
...H);theDirectorateofDiabetesMetabolism,RoyalLondonUniversityHospital,London(PGK);theObesityUnit,HuddingeUniversityHospital,Huddinge,Sweden(SR);andtheRowettResearchInstitute,Aberdeen,UnitedKingdom(WPJ).2SupportedbyKnollPharmaceuticals,Nottingham,UnitedKingdom.3AddressreprintrequeststoMAvanBaak,Departm...
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與腎臟發生相關的基因研究進展
...合在腎小球發生中的作用[15],Soriano[16]將lacZ基因與pgk-neo基因相連接以觀察PDGFβ基因啓動部位在雜合子變異體上的表達。PDGFβ基因在腎小球發生早期表達在輸尿管芽,在發生後期主要侷限於系膜細胞。 PDGFB與PDGFR基因變...
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HypoxicinductionofCtgfisdirectlymediatedbyHif-
...lability(VEGF,erythropoietin)andenhancingglucoseuptakeandmetabolism(Glut-1,PGK).Inthispaper,wehaveusedprimarytubularepithelialcellculturesfromatetracycline-inducible-Hif-1knockoutmurinemodeltofurtherelucidatetheroleofHif-1inthehypoxic-inductionofCtgfexpression.Weshowthathypoxiaresponseelementspresen...
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絲狀真菌瑞氏木黴生產重組蛋白的分子生物學研究進展
...報道從瑞氏木黴中分離了非纖維素酶基因的啓動子,包括pgk啓動子、pkiA啓動子、tefl啓動子和一個在葡萄糖培養基上強表達但未知的cDNA1的啓動子,這些啓動子可以在葡萄糖培養基上啓動合成所需要的纖維素酶,產生的酶製劑基...