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肥大細胞對THP-1巨噬細胞源性泡沫細胞膽固醇分配和流出的影響
...Valuesarerepresentedas()mgcholesterol/gprotein,(n=5).a:P0.05vs.control,HDL3+MCsgroup;b:P0.05vs.controlgroup;c:P0.05vs.HDL3group.CholesterolstandardcontrolHDL3MCsHDL3+MCsC48/80HDL3+C48/80ThechromatographypictureofcholesterolandcholesterolesterinTHP-1macrophage-derivedfoamcellsidentifiedbyhighperf...
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高密度脂蛋白抗動脈粥樣硬化機制—SR-BI,CD36,PPARgamma的作用
...SR-BI、CD36表達上調,主動脈組織PPARγ表達上調。HDL2和HDL3對THP-1巨噬細胞SR-BI、CD36和PPARγ表達的影響第二部分HDL2和HDL3對THP-1巨噬細胞中SR-BI表達的影響HDL2和HDL3處理經OxLDL誘導後細胞中SR-BImRNA的表達變化細胞中SR-BImRNA表達變...
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HDL3InducesCyclooxygenase-2ExpressionandProstacyclinReleaseinHumanEndothelialCellsViaap38MAPK/CRE-DependentPathway:EffectsonCOX-2/PGI-Syntha
...ecularmechanismsinvolvedinthiseffectinendothelialcells.MethodsandResults—HDL3(30μg/mL)inducedCOX-2expressioninatime-anddose-dependentmanner.COX-2wasfoundmainlyintheperinuclearareawhereitco-localizeswithPGIsynthase.TransienttransfectionexperimentsshowedthatCREisrequiredforHDL-inducedCOX-2transcrip...
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膽固醇酯轉移蛋白與動脈粥樣硬化
...0±0.26μg/ml,女性爲1.92±0.52μg/ml。在血漿中CETP主要存在於HDL3和VLDL,電泳時分佈於α2條帶上。人類、兔、猴子和蒼鼠的CETP基因cDNA已被克隆。由克隆的人類CETP基因cDNA序列推導出人CETP內含17個氨基酸組成的信號肽和476個氨基酸組成...
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MacrophagecholesteroleffluxelicitedbyhumantotalplasmaandbyHDLsubfractionsisnotaffectedbydifferenttypesofdietaryfattyacids
...turated,orsaturatedFAs.ThesedietaryeffectsonthecompositionandabilityofHDL2,HDL3,andtotalplasmatoeffluxcholesterolfrommouseperitonealmacrophagesthatpreviouslywereloadedwithLDL-acetylated14C-cholesterylesterwereevaluatedbyusingultracentrifugallyisolatedlipoproteins.Design:Aftera2-wkrun-inperiod,30heal...
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第五節 脂蛋白代謝
...、脂肪組織及心肌組織貯存或利用。CM表面的磷脂和Apo往HDL3移行,顆粒變小,結果轉變成CM殘粒,分別被肝臟LDL受體和清道夫受體識別並攝取。 CM生理功能是轉運外源性脂類,主要是甘油三酯。其中TG在毛細血管中被水解成遊...
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脂蛋白脂肪酶基因與動脈粥樣硬化
...含量下降;患者血漿HDL亞類含量改變,HDL2b顯著降低;HDL2a及HDL3a增加,且隨血漿含量的升高而升高,HDL3b及HDL3c則未見改變。與正常人比較,Ⅳ型高脂血症患者血清中小顆粒的前β1-HDL和HDL3a含量顯著增加,而成熟的HDL2b含量顯著減...
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AssociationofCholesterolSubfractionsandCarotidLipidCoreMeasuredbyMRI
...itylipoprotein(HDL),comprisedprimarilyof2subfractions,HDL2(largebuoyant)andHDL3(smalldense),hasaprotectiveroleinCHD.4,5LDLcanbedenseorbuoyant,anddenseLDLishighlyatherogenic,associatedwith4-foldincreasedCHDrisk.6Lipoproteina,isastrongriskfactorforCHDandstroke.7MRIcannoninvasivelyvisualizearterialwall...
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PreferentialSphingosine-1-PhosphateEnrichmentandSphingomyelinDepletionAreKeyFeaturesofSmallDenseHDL3Particles
...opulationsandpotentialrelevancetoantiatherogenicbiologicalactivitiesofdenseHDL3.MethodsandResults-Themolecularprofileoflipids(cholesterylesters,phospholipids,sphingomyelin,andsphingosine-1-phosphate)inphysicochemically-definednormolipidemicHDLsubpopulationswasdeterminedbyhigh-performanceliquidchroma...
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第四節 血清脂蛋白非電泳定量法
...不受影響。 三、高密度脂蛋白亞組份膽固醇(HDL2-C,HDL3-C)檢測 聚乙二醇20000沉澱法 1.原理: HDL中主要組份爲HDL2和HDL3。用聚乙二醇20000(PEG20000)作沉澱劑,以不同濃度在不同pH條件下,可將HDL2和HDL3分離開。95...
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Fish-oilestersofplantsterolsimprovethelipidprofileofdyslipidemicsubjectsmorethandofish-oilorsunfloweroilestersofplantsterols
...2).FO-PSandSU-PSresultedinsimilarratiosofapoBtoapoA.HDL2andtheratioofHDL2toHDL3weresignificantlyhigherattheendoftheFO-PStreatmentthanattheendoftheOO(P=0.0006),fishoil(P=0.0036),andSU-PS(P=0.0016)treatments.Conclusion:SupplementationofanOO-baseddietwithFO-PSmayreducecardiovasculardiseaseriskmorethand...
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AntioxidativeActivityofHDLParticleSubspeciesIsImpairedinHyperalphalipoproteinemia:RelevanceofEnzymaticandPhysicochemicalProperties
...ndResults—GradientfractionationrevealedthatbuoyantHDL2band2aandsmalldenseHDL3band3clevelswereelevatedupto2.0-foldinHALPsubjects(n=9;meanplasmaHDLcholesterol,79mg/dL)withlowhepaticlipaseactivity.HDL2a,3a,3b,and3cdisplayedlowerspecificantioxidativeactivity(sAA)duringlow-densitylipoprotein(LDL)oxidat...
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冠心病的危險因素調查
...固醇(HDL2-C)≤0.16mmol/L、高密度脂蛋白亞組分3膽固醇(HDL3-C)≤0.67mmol/L、低密度脂蛋白膽固醇(LDL-C)≥3.60mmol/L、極低密度脂蛋白膽固醇(VLDL-C)≥0.90mmol/L、TC≥4.68mmol/L、TG≥1.30mmol/L、ApoB/ApoA≥0.70、LDL-C/HDL-C≥3.35和(或)HDL-...
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第二節 血脂及其代謝
...分子中蛋白與脂類成分有變化,可將HDL再分爲HDL1、HDL2與HDL3。HDL1是在高膽固醇膳食時纔出現,HDL2爲成熟的HDL,HDL3爲新生的HDL,其分子中蛋白成分多。 血漿中的遊離中短鏈脂肪酸可與血漿白蛋白結合而被運輸,稱之爲脂酸白...
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附錄一參考值
...沉澱法) HDL2-C男1.16-0.72mmol/L** 女0.19~0.75mmol/L HDL3-C男0.42~1.08mmol/L 0.44~1.06mmol/L **低密度脂蛋白膽固醇(LDL-C) 直接遮敝法或聚陰離子沉澱法2.07~3.11mmol/L(男女)**極低密度脂蛋白膽固醇(VLDL-C) 電...
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InhibitionofCholesterylEsterTransferProteinActivitybyJTT-705IncreasesApolipoproteinE–ContainingHigh-DensityLipoproteinandFavorablyAffectsthe
...itedCETPactivities,increasedHDLcholesterol(HDL-C)levelsandtheratioofHDL2-C/HDL3-C,anddecreasedthefractionalesterificationrateofcholesterolinHDL,indicatingpreferentiallyincreasedlargeHDLparticles.TreatmentwithJTT-705increasedallofthe3charge-basedHDLsubfractionsasdeterminedbycapillaryisotachophoresis:...
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第二節 高脂蛋白血癥
...HDL增多;若HTGL活性降低,HTGL與HDL被肝細胞攝取並使HDL2→HDL3轉換過程有關。HTGL活性降低使HDL被肝細胞攝取量減少而停留在血液中並使其濃度增加,出現高HDL血癥。血清中總膽固醇輕度或中度升高,HDL-C高達正常人3~5倍的高值,...
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內皮脂肪酶與血脂和冠心病的關係
...在對加拿大人羣的研究中發現,Thr111Ile突變純合子的女性HDL3-C水平高於未突變組,且富含不飽和脂肪酸的飲食可導致Thr111Ile突變純合子的女性HDL3-C水平升高,而正常純合子女性則出現HDL3-C水平下降,其原因可能是突變降低了EL的...
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Puttingcholesterolinitsplace:apoEandreversecholesteroltransport
...ackagedintothehydrophobiccoreofthediscoidalparticle,convertingittosphericalHDL3.HDL3cancontinuetoacceptunesterifiedcholesterolandphospholipidsfromtheclassB,typeIscavengerreceptor(SR-BI);throughcontinuedactionofLCAT,thecoreexpandsandtheparticlesizeincreases,formingHDL2.Althoughthemajorapolipoproteinc...
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第六節 載脂蛋白D
...HDL中僅次於ApoAⅠ、AⅡ的一種載脂蛋白,在HDL2中約佔21%,HDL3中佔43%,VLDL中含有36%,ApoD化學本質是糖蛋白,其糖的組份中含有葡萄糖,甘露糖、半乳糖、葡萄胺和唾液酸。ApoD又稱爲薄線肽(thinlinepeptide),其分子量爲22100,爲一...
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第四節 高密度脂蛋白代謝
...用下,遊離膽固醇變成膽固醇酯,脂蛋白則變成成熟球形HDL3’,再經LPL作用轉變成HDL2。 HDL可將蓄積於末稍組織的遊離膽固醇與血循環中的脂蛋白或某些大分子結合而運送到各組織細胞,主要是肝臟,這種膽固醇被利用的過...
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血脂異常的診治進展
....021.101.101.20204060801000密度(g/ml)直徑(nm)VLDL510IDLVLDL乳糜殘粒HDL3HDL2乳糜微粒LDL脂蛋白脂蛋白直徑(?)密度(g/ml)主要脂質成分載脂蛋白乳糜微粒800–50000.94食物中甘油三酯A-I,A-II,A-IV,B-48,C-I,C-II,C-III,EVLDL300–8000.94–1.006內源性甘油三酯B-100...
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DecreasedHigh-DensityLipoprotein(HDL)ParticleSize,Preß-,andLargeHDLSubspeciesConcentrationinFinnishLow-HDLFamilies
...in(aspercentage)ofintegrationareaofHDL2bwassignificantlylower,whereasthatofHDL3a,HDL3b,andHDL3cwassignificantlyhigherintheaffectedfamilymembersthanintheunaffectedfamilymembersandinthecontrolgroup.ThegreatestdifferencewasdemonstratedinHDL2b,withareductionof&50%intheaffectedfamilymemberscomparedwithth...
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第四節 脂蛋白代謝紊亂與動脈粥樣硬化的相關性
...和高密度脂蛋白(HDL)。其中HDL又分爲兩個亞類即HDL2和HDL3。若一種或幾種脂蛋白的結構和代謝發生異常,則可能引起AS。 控制脂蛋白代謝和轉運的主要有①載脂蛋白(Apo),包括AⅠ、AⅡ、AⅣ、B100、CⅠ、CⅡ、CⅢ、D、E和Ap...
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第三章 載脂蛋白的種類與結構--第一節 載脂蛋白A族
...生理功能是非常重要的。 ApoAⅠ主要存在於HDL中,佔HDL3載脂蛋白的65%,佔HD2載脂蛋白的62%。在CM、VLDL和LDL中也有少量存在。血漿中呈現β遷移率的一種β-HDL,其中有80%的ApoAⅠ。 ApoAⅠ的生理功能有:①組成脂蛋白並維持其...
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第六節 脂蛋白代謝紊亂
...多;若HTGL活性降低,HTGL與HDL被肝細胞攝取減少並使HDL2→HDL3轉換過程減慢而停留在血液中,並使其濃度增加,出現高HDL血癥。血清中總膽固醇輕度或中度升高,HDL-C高達正常人3-5倍,血清ApoAⅠ、CⅢ、E明顯增加,ApoB呈低值。因...
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血脂代謝異常
...種脂蛋白及載脂蛋白又可分爲若干亞型(如HDL可有HDL2、HDL3等亞型,Apo-A可分Apo-AI、Apo-AII等,Apo-B可分爲Apo-B100和Apo-B48等,Apo-C有Apo-CI、Apo-CⅡ、Apo-CⅢ等),血脂、脂蛋白、載脂蛋白在人體代謝中起着重要的作用。人體內血液中...
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Phosphatidylinositol-3-KinaseRegulatesScavengerReceptorClassBTypeISubcellularLocalizationandSelectiveLipidUptakeinHepatocytes
...eshmediumandcellswereusedforexperiments.HDLIsolationandRadiolabelingHDL2andHDL3wereisolatedbysequentialultracentrifugationfromhumanplasmaasdescribedpreviously.22HDL3wasdouble-labeledbyradioiodinationoftheproteincomponentusing125Iandbytracingthecholesterolestercomponentwithnonhydrolyzable[1,2(n)-3H]c...
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第二節 脂蛋白的化學組成
...漿脂蛋白的化學組成總脂蛋白的重量百分比 CMVLDLLDLHDL2HDL3蛋白質2.010214155磷 脂7.5182229.522.5膽固醇酯7.7123516.211.7遊離膽固醇0.8785.42.9甘油三酯88.05394.54.1載脂蛋白的百分構成比(%)載脂蛋白 AⅠ+--65AⅡ---25B 4095- CⅠ10-...
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Effectof6dietaryfattyacidsonthepostprandiallipidprofile,plasmafattyacids,lipoproteinlipase,andcholesterolestertransferactivitiesinhealth
...GmbH,Mannheim,Germany)onaCobasMiraanalyzer(Roche,Basel,Switzerland).HDL-andHDL3-cholesterolconcentrationsweremeasuredenzymaticallyafterprecipitationwithpolyethyleneglycol(Quantolip;ImmunoAG,Vienna)(35).HDL2cholesterolwascalculatedasthedifferencebetweentotalHDLcholesterolandHDL3cholesterol.LDLcholest...