RNA提取實驗方法流程
...丙醇和乙醇, 5)、mRNA分離試劑盒:OligotexmRNAMiniKit,Cat.no.:70022,QIAGEN。 6)、新配的電泳緩衝液,專跑RNA的瓊脂糖(Agarose)。 3、實驗流程 3.1細胞總RNA的提取 1)、6孔板細胞(CNE-2)匯合度爲90-100%時,取出無...
醫藥經濟;生物技術;實驗技術;生化與分子技術CompleteRescueofLipoproteinLipase–DeficientMicebySomaticGeneTransferoftheNaturallyOccurringLPLS447XBeneficialMutation
...ucedriskofcoronaryarterydisease.TheS447XvariantmaybeassociatedwithhigherLPLcatalyticactivity;however,invitrodatasupportingthishypothesisarecontradictory.WewantedtoinvestigatetheinvivomechanismbywhichtheLPLS447XvariantimprovesthelipidprofileofS447Xcarriers.WeconductedafunctionalassessmentofhumanLPLS4...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第10期高親和力IgE受體α鏈基因亞型的發現及其cDNA序列的克隆
...第159位密碼子由CAC置換成了CGC,185位密碼子由TAT置換成了CAT。6例血標本來源的RT-PCR產物,經SSCP分析表明,其559bp擴增片段遷移率完全一致,結合測序結果,該實驗證明我們所檢測的6例正常個體,其FcεR1膜外區α鏈cDNA的第159和185...
合作平臺;醫學論文;臨牀醫學與專科論文;檢驗醫學ACAT2IsaTargetforTreatmentofCoronaryHeartDiseaseAssociatedWithHypercholesterolemia
...t.2005;25:923–931.AbstractTheinhibitionofintracellularcholesterolesterificationasameanstopreventatherosclerosishasbeenconsideredtohavepotentialformanyyears.TwodifferentACATenzymeswerediscoveredabout7yearsago,andithasbecomeclearthatthetwoenzymesprovideseparatephysiologicfunctions.Muchhasbeenlearned...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第6期十二種無血清培養基的資料
...白濃度大約爲3mg/ml。可在UltraCULTURE培養基中添加防凍劑(Cat.No.12-132),用於無血清環境下細胞的凍存。UltraCULTURE培養基不含L-穀氨酸,使用前請先加入5ml200mM的L-穀氨酸溶液(Cat.No.17-605或17-905)產品貨號:12-725FUltraCULTUREMEDIUM(Serumfreege...
藥品天地;專業藥學;實驗技術;基本實驗技術;常用試劑Adiposetissuebiomarkersoffattyacidintake
...uen-6fattyacidswerehighlycorrelated:18:2(r=0.58)and18:3(r=0.24).Thebestindicatorsoftotaltransfattyacidintakewerect18:2n6andtc18:2n6(r=0.58foreach);total18:1transfattyacid(r=0.45)and16:1transfattyacid(r=0.16)werethenextbestindicators.Conclusions:Adiposetissueisasuitablebiomarkerofdietaryf...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2002年76卷第4期版納園揭示超低溫保存對頑拗性種子的細胞學和生理學影響
...甚小,但是使抗壞血酸過氧化物酶(APX)與過氧化氫酶(CAT)的活性明顯降低。超低溫處理能夠進一步降低抗壞血酸過氧化物酶(APX)和谷胱甘肽還原酶(GR)的活性,然而在脫水後卻能夠提高過氧化氫酶(CAT)的活性。在凍-融...
醫藥經濟;生物技術;技術要聞GeneExpressionChangesduringtheDevelopmentofAcuteLungInjuryRoleofTransformingGrowthFactor
...oor,withmortalityat20to40%;and,ifresolutionoccurs,persistentpulmonarycomplicationsdevelop,includinginterstitialfibrosisanddiminishedlungcompliance.Transforminggrowthfactor(TGF-)existsinthreeisoformsandisamemberofafamilyofgrowthfactorsthatisabletomodulateseveralcellularprocessesinmultipleorgansystems...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2005年第171卷第12期MMP-12與動脈粥樣硬化
...aIntimaIntimaLesionsNormalAortaNorthernblotNon-lesionLesionNormalPro-MMP-12CatalyticMMP-12IsolatedfoamcellsConditionedmedium66.342.4WesternblotMatsumoto,S.AmJ.Pathol.1998HypothesisDoesMMP-12playacriticalroleinatherogenesis,plaquerupture,andaneurysm?TransgenicrabbitmodelsVS.Sensitivetoacholesteroldie...
醫源資料庫;醫學文檔庫;心血管相關HepatitisCVirusCoreProteinDown-RegulatesTranscriptionofInterferon-InducedAntiviralGenes
...ylatesynthetase(25OAS)mRNAweredetermined.Chloramphenycolacethyltransferase(CAT)analysiswasperformedoncellscotransfectedwithpHCV-CoandpMx4CAT(containingtheMxAgenepromoter)andtreatedwithIFN.Electrophoreticmobilityshiftassayswereused,andWestern-blotanalysisofSTAT1and2wasperformed.Results.LevelsofMxAmRN...
醫源資料庫;在線期刊;傳染病學雜誌;2005年第191卷第1期