HighPrevalenceofRespiratoryViralInfectionsinPatientsHospitalizedinanIntensiveCareUnitforAcuteRespiratoryInfectionsasDetectedbyNucleicAci
...anotshown).Inthepresentseriesofseverepneumonia,viralinfectionsweredetectedbyusingnucleicacid-baseddiagnosisproceduresinBALsamplesfromnearlyone-thirdofthepatients;whenonlypatientswithoutbacterialetiologywereconsidered,two-thirdsofsampleswerefoundtobepositiveforrespiratoryvirus.Themolecularassaysweuse...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第1期PeritonitisDuetoCurvulariainaequalisinanElderlyPatientUndergoingPeritonealDialysisandaReviewofSixCasesofPeritonitisAssociatedwithOtherC
...tosine,andDeVaultetal.(6)gavenoantifungaltreatment.Brackettetal.(3)initiallyusedi.p.amphotericinBwithoutsuccessandchangedtoi.v.amphotericinB.Inallcases,thedialysiscatheterwasultimatelyremoved.Catheterremovalwasatemporarymeasureinfourcases.Ujhelyietal.(20)andLopesetal.(15)optedfortheirpatientstoremai...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期NO/redoxdisequilibriuminthefailingheartandcardiovascularsystem
...chanismsbywhichRNSandROSmodulatecellularsignaltransductionremainincompletelyunderstood,thereisageneralconsensusthatcysteineresiduesareprincipalsitesofredoxregulation(10,13,14).CrosstalkbetweenROS-andRNS-regulatedpathwaysmayoccuratboththechemicalinteractionlevel(15,16)andthroughtheircoordinateeffects...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第3期Ikarosintegratesendocrineandimmunesystemdevelopment
...GAA-substitutedby-CGTTAA-intothePOMCpromoterfragmentP(–543)-Lucconstructbyusingmutagenicprimersforeachofthe5potentialIkaros-bindingsitesidentifiedinthispromoter(Figure2A).Allmutationswereconfirmedbynucleotidesequencing.Transfectionandluciferaseassays.Plasmidreporterswerepreparedbycolumnchromatogra...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第4期EvaluationofReal-TimeReverseTranscriptasePCRandReal-TimeLoop-MediatedAmplificationAssaysforSevereAcuteRespiratorySyndromeCoronavirusDetection
...ofthereal-timeLAMPassayandahighlysensitivereal-timequantitativeRT-PCRassaybyusingretrospectiveclinicalspecimens.Inthisstudy,59retrospectivenasopharyngealaspirates(NPA)fromSARSpatientswererecruitedinthisstudy.AllofthesepatientswereconfirmedtobeSARSpatientsbyserologicalorPCRtestsasdescribedbefore(16)....
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第7期Roleofcaveolin-1intheregulationofthevascularshearstressresponse
...av-1mayregulatethevascularresponsetoalteredshearstress.InthisissueoftheJCI,Yuetal.haveexaminedtheroleofCav-1/caveolaeintheregulationofflow-inducedalterations(i.e.,mechanotransduction)invesselsfromwild-typemice,Cav-1–deficientmice,andCav-1–deficientmicere-expressingCav-1onlyinECs.Theirdatasuggest...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第5期SignificanceofTransientlyPositiveEnzyme-LinkedImmunosorbentAssayResultsinDetectionofHelicobacterpyloriinStoolSamplesfromChildren
...terol.Nutr.26:393-397.Foley,J.E.,S.L.Marks,L.Munson,A.Melli,F.E.Dewhirst,S.Yu,Z.Shen,andJ.G.Fox.1999.IsolationofHelicobactercanisfromacolonyofbengalcatswithendemicdiarrhea.J.Clin.Microbiol.37:3271-3275.Fox,J.G.1997.TheexpandinggenusofHelicobacter:pathogenicandzoonoticpotential.Semin.Gastrointest.Dis...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第5期DetectionofSevereAcuteRespiratorySyndromeCoronavirusRNAinPlasmaduringtheCourseofInfection
...wellassequentialchangesinSARS-CoVloadintheplasmaofadultpatientsremainlargelyunclear.Inthisstudy,weexaminedSARS-CoVRNAinplasmafrompatientsofprobableSARScasesduringthecourseofinfectionbyusingapreviouslydescribedquantitativereal-timereversetranscriptionPCR(RT-PCR)assay(14).Moreover,weinvestigatedsequen...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第2期基因或決定腦容量與語言能力
...,因此無證據表明MCPH1和ASPM基因仍在受到正向選擇。F.L.Yu等人則認爲ASPM變異模式並不獨特,它是中性突變,並未經受正向選擇。N.Mekel-Bobrov等人雖兩次對上述質疑從研究方法和數據採用等方面予以了反駁,如Currat等人所採用的人...
醫藥經濟;生物技術;技術要聞ComparativeEvaluationofReal-TimePCRAssaysforDetectionoftheHumanMetapneumovirus
...ificationoftheamplicons(FandNgenesonly).AsshowninTable2,thereal-timePCRassayusingprimerscomplementarytotheNgenesuccessfullyamplifiedviralRNAfromall20putativehMPVcultureswhereasthePCRpositivityratewassomewhatlowerfortheothergenes,i.e.,15of20(75%),11of20(55%),12of20(60%),and18of20(90%)withtheM,F,P,and...
醫源資料庫;在線期刊;微生物臨牀雜誌;2003年第41卷第8期