PI3Kintegratestheactionofinsulinandleptinonhypothalamicneurons
...shington,USA.AbstractCentralcontrolofenergybalancedependsontheabilityofproopiomelanocortin(POMC)oragouti-relatedprotein(Agrp)hypothalamicneuronstosenseandrespondtochangesinperipheralenergystores.Leptinandinsulinhavebeenimplicatedascirculatingindicatorsofadiposity,butitisnotclearhowchangesintheirleve...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第4期質粒DNA促進HBsAg-抗HBs複合型疫苗誘生的免疫應答的研究
...免疫應答的類型。方法 分別以HBsAg、HBsAg-抗-HBs(IC)、pI/AmpHBs、IC-pI/AmpHBs及IC-pI/Amp免疫小鼠,檢測抗-HBs的效價,分析抗-HBsIgG亞類(ELISA);取免疫小鼠脾細胞,體外抗原刺激,用競爭性RT-PCR方法檢測IFN-γ及IL-4mRNA轉錄水平。結...
合作平臺;醫學論文;臨牀醫學與專科論文;檢驗醫學PTEN基因轉染對人膀胱癌細胞BIU87中PI3KAkt信號通路的影響
...摘要】目的研究抑癌基因PTEN轉染對人膀胱癌細胞BIU87中PI3KAkt信號通路的作用。方法將攜有PTEN基因的重組真核表達質粒pBpPTEN轉化大腸桿菌DH5α並擴增,抽提純化質粒並進行酶切鑑定,pBpPTEN體外轉染BIU87細胞(pBpPTENBI...
醫源資料庫;在線期刊;現代泌尿外科雜誌;2008年第12卷第5期Parathyroidhormone-dependentendocytosisofrenaltypeIIcNa-Picotransporter
...osphatemicricketswithhypercalciuriaresultsfrommutationsoftherenaltypeIIcNa-Picotransportergene,suggestingthatthetypeIIctransporterplaysaprominentroleinrenalphosphatehandling.ThegoalofthepresentstudywastoinvestigatetheregulationofthetypeIIcNa-Picotransporterbyparathyroidhormone(PTH).TypeIIcNa-Picotra...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第1期Centralcontrolofrenalsodium-phosphate(NaPi-2)transporters
【摘要】Regulationofphosphate(Pi)reabsorptionoccursthroughtheup-anddownregulationoftherenaltype-IIsodiumPicotransporters(NaPi-2).Recently,renalNaPi2-typeexpressionhasbeenidentifiedinareasofthebrain.ThepresentstudydeterminedwhetherbrainNaPi-2isregulatedbydietaryPiandwhetherthebehavioralandrenalad...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第4期II型α亞型磷脂酰肌醇-4-激酶的結構功能研究獲得突破
圖a,hPI4KIIα催化核心ADP結合狀態整體結構;圖b,ATP結合口袋ADP與hPI4KIIα的相互作用;圖c,ATP結合口袋重要氨基酸殘基突變體激酶活性;圖d,基於分子動力學模擬推測的PI結合口袋;圖e,棕櫚酰化插入片段突變體激酶活性測定...
醫藥經濟;生物技術;技術要聞藥物分析技術
...對乙酰氨基酚的分析二、鹽酸普魯卡因的分析習題第八章磺胺類藥物的分析第一節磺胺類藥物的結構特點第二節磺胺類藥物的主要化學鑑別法一、重氮化-偶合反應二、銅鹽反應第三節磺胺類藥物的紅外光譜鑑別法一、磺胺類藥...
醫源資料庫;醫源書店;藥學UltrasoundStimulatesCyclooxygenase-2ExpressionandIncreasesBoneFormationthroughIntegrin,FocalAdhesionKinase,Phosphatidylinositol3-Kinase,andAkt
...tegrin,attenuatedtheUS-inducedCOX-2expression.Phosphatidylinositol3-kinase(PI3K)inhibitors2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-onehydrochloride(LY294002)andwortmanninalsoinhibitedthepotentiatingactionofUS.USstimulationincreasedthephosphorylationoffocaladhesionkinase(FAK),extracellularsignal...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第6期EssentialRoleforClassIIPhosphoinositide3-kinase-IsoforminCa2+-Induced,Rho-andRhoKinase-DependentRegulationofMyosinPhosphataseandContraction
...e-silencingtechnique,allowedustodeterminetheroleofphosphoinositide3-kinase(PI3K)classII-isoform(PI3K-C2)asanovel,Ca2+-dependentregulatorofmyosinlight-chainphosphatase(MLCP)andcontraction.TheCa2+-ionophoreionomycininducedarobustcontractileresponsewithanincreaseintheintracellularfreeCa2+concentration(...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第3期CorrelationbetweenhyperphosphatemiaandtypeIINa-Picotransporteractivityinklothomice
...phatehomeostasis.ThegoalofthepresentstudywastoinvestigatetheregulationofNa-Picotransportersinklothomutant(kl/kl)mice.Thekl/klmicedisplayedhyperphosphatemia,highplasma1,25(OH)2D3levels,increasedactivityoftherenalandintestinalsodium-dependentPicotransporters,andincreasedlevelsofthetypeIIa,typeIIb,andt...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第2期