SegmentalexpressionofNotchandHairygenesinnephrogenesis
...ugatedtoFITCwereusedtodetectcollectingductsandproximaltubules.Peanutlectin(PNA)conjugatedtorhodaminewasusedtodetectglomerularpodocytes.Toperformlectinstaining,weperformedinsituhybridizationonsectionsasdescribedabove.SlideswerethenwashedthreetimeswithPBS,treatedwithgelatinbuffer(0.075%saponindetergen...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第5期Extracellularmatrixregulatesglomerularepithelialcellsurvivalandproliferation
...pase-8and-9activitiesweremeasuredusingacolorimetricassaywithp-nitroaniline(pNA)-labeledsubstrates,accordingtothemanufacturer‘sinstructions.Adherentcellswerelysedinbuffercontaining50mMHEPES,100mMNaCl,0.1%CHAPS,1mMDTT,and100µMEDTA,pH7.4(4°C).Themixturewascentrifugedat14,000gfor10min.Supern...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第2期LossofIRF-4–bindingproteinleadstothespontaneousdevelopmentofsystemicautoimmunity
...eBcellpopulationsintheIBPtrap/trapmicedemonstratedamarkedincreaseintheB220+PNAhipopulation(datanotshown)andintheB220intsyndecan-1+plasmacellpopulationbutnoincreaseinB-1cells(Figure3Canddatanotshown).Interestingly,FACSanalysisofthebonemarrowoftheseanimalsrevealedamarkeddepletionofallBcellsubsetsandno...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第3期HIV-1轉錄反式激活抑制劑研究爲艾滋病治療拓新路
...定的複合物。美國研究人員發現,聚酰胺核苷酸類似物(PNA)與TAR的環和凸起區互補結合,阻斷Tat-TAR複合物的生成進而抑制HIV-1複製。 靶向於TAR的Tat擬肽類抑制劑 競爭Tat-TAR相互作用的肽或肽模擬物可以阻斷HIV-1的複製。此...
醫學教育;科教新聞TheToxicityofthePrP0-PrionPeptideonCulturedPhotoreceptorsCorrelateswiththePrionProteinDistributionintheMammalianandHumanRetina
....Louis,MO),whereasconephotoreceptorswerestainedbythepeanutlectinagglutinin(PNA,1:40;Sigma).ApoptoticcellswerelabeledwithterminaldeoxynucleotidaltransferasedUTPnick-endlabeling(TUNELkit;RocheDiagnostics,Basel,Switzerland).4,6-Diamidino-2-phenylindolenucleardyewasappliedinPBSfor2minutes.Finally,cellsw...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第4期EnhancedBCellExpansionSurvivalandHumoralResponsesbyTargetingDeathReceptor6
...ofeitherbiotinylatedanti–mouseCD45R/B220()orbiotinylatedpeanutagglutinin(PNA;)wasaddedtotheslidesandincubatedfor60min.Streptavidin–horseradishperoxidasekit(LSAB2;DakoCytomation)wasthenusedalongwitha3,3‘-diaminobenzidinechromagen.Slideswereremovedfromtheautostainerandcounter-stainedwithhematoxy...
醫源資料庫;在線期刊;美國病理學雜誌;2003年第162卷第1期EnhancedBCellExpansion,Survival,andHumoralResponsesbyTargetingDeathReceptor6
...ofeitherbiotinylatedanti–mouseCD45R/B220()orbiotinylatedpeanutagglutinin(PNA;)wasaddedtotheslidesandincubatedfor60min.Streptavidin–horseradishperoxidasekit(LSAB2;DakoCytomation)wasthenusedalongwitha3,3‘-diaminobenzidinechromagen.Slideswereremovedfromtheautostainerandcounter-stainedwithhematoxy...
醫源資料庫;在線期刊;實驗醫學雜誌;2003年第197卷第1期大腸癌轉移相關基因骨橋蛋白反義及正義真核表達質粒的構建
...ticexpressionplamidsprovideexperimentalplatformforfollowinginvestigationonOPNandmetastasisincolorectalcarcinoma. Keywordscolorectalneoplasmosteopontinplasmid 骨橋蛋白(osteopontin,OPN)基因定位在人類基因組的4q13和大鼠第5個染色本的ric基因上。人OPN基因cDNA...
資料庫;在線期刊;中華現代外科學雜誌;2004年第1卷第1期腎臟黏液性管狀及梭形細胞癌的臨牀病理觀察
...表達,而集合管上皮標記物EMA和花生凝集素(peanutagglutinin,PNA)卻在MTSCC中有好的表達[4],由此他們認爲該腫瘤起源於集合管。但Paner等的研究發現絕大多數的MTSCC近曲小管相關的標記物AMACR呈陽性表達,並不支持遠端腎單位來源說[6]...
醫源資料庫;在線期刊;中華現代外科學雜誌;2010年第7卷第4期急性腎小管壞死
...之比/尿肌酐、血肌酐之比)×100,即:FeNa(%)=UNaV÷GRF×100PNa=UNa·V÷UCr·V×100PNaPCr=UNa×PCr×100PNaUCrUNa爲尿鈉,PNa爲血鈉,V爲尿量,UCr爲尿肌酐,PCr爲血肌酐,GFR爲腎小球濾過率。ATN患者常>1,腎前性少尿者則常<1。上述⑤至⑨尿...
求醫問藥;疾病手冊;腎病內科