Variabilityinconversionofß-carotenetovitaminAinmenasmeasuredbyusingadouble-tracerstudydesign

...Weusedadouble-tracertest-retestdesign.Wedosed11healthymenorallywith30µmolhexadeuterated(D6)retinylacetate(all-trans-19,19,19,20,20,20-[2H6]retinylacetate)andthenwith37µmolD6ß-carotene(19,19,19,19‘,19‘,19‘-[2H6]ß-carotene)1wklater.Dosesweretakenwithbreakfastscontaining16gfat...

上海市2525名中學生血清尿酸含量檢測結果分析

...定。結果2525名學生平均血清尿酸水平男生爲(315.1±65.5)μmol/L，女生爲(266.4±58.9)μmol/L，其中有164例血清尿酸含量高於390μmol/L(6.50%)，78例血清尿酸含量高於420μmol/L(3.09%)，39例血清尿酸含量高於500μmol/L(1.54%)。結論將尿酸作爲學生體...

活性氧在血管緊張素Ⅱ誘導ECV304細胞增殖中的作用

...胞產生ROS（·OH）的量。結果一定濃度的AngⅡ（0.03125～1μmol/L）作用12h時ECV304細胞增殖率增加，明顯高於正常對照組（P0.05）;AngⅡ可誘導ECV304細胞產生ROS（·OH），ROS（·OH）的含量與ECV304細胞增殖率呈顯著負相關;10mmol/LNAC可抑制1μ...

EffectsofDidanosine-RelatedDepletionofmtDNAinHumanTLymphocytes

...ionoflactate.InCD4lymphocytes,10-dayincubationwithddIatconcentrationsof11.8mol/L,35.4mol/L,59.0mol/L,and118.0mol/Linduced(1)aconcentration-dependentreductionofbothmtDNA(to73%,29%,24%,and23%,respectively,ofthelevelsincontrolsamples)andsubunitIIofmtDNA-encodedcytochromecoxidase(to86%,81%,55%,and31%,re...

ConstrictorandDilatorEffectsofAngiotensinIIonCerebralArterioles

...tsofAngIIonratpialarterioles.Methods—WedeterminedtheeffectofAngI(3.10–6mol/L)intheabsenceandpresenceoftheconvertingenzymeinhibitor,captopril(10–5mol/L)incerebralarteriolesofmaleWistarrats(open-skullpreparation),andthoseofAngII(3.10–12to3.10–6mol/L)intheabsenceandpresenceoftheAngIIreceptor(...

MethylenetetrahydrofolateReductasePolymorphismsandHomocysteine-LoweringEffectofVitaminTherapyinSingaporeanStrokePatients

...olymorphisms.Results—MeanbaselinetHcywassimilarinthe2groups(placebo13.7μmol/L;vitamins14.0μmol/L;P=0.70).At1year,meantHcywas14.5μmol/Lintheplacebogroupcomparedwith10.7μmol/Linthevitamingroup(difference3.8μmol/L;95%CI,2.8to4.8μmol/L;P0.0001).MTHFRC677TgenotypewasanindependentdeterminantoftHcy...

鋁碳酸鎂片

...別：取細粉適量（約相當於鋁碳酸鎂1.0g），加鹽酸液（2mol/L）20ml，振搖，使溶解，濾過，於濾液中加水30ml，煮沸，加氨液（2mol/L）至對甲基紅呈鹼性，繼續煮沸2分鐘，濾過。沉澱用熱氯化銨溶液（2%W/V）50ml洗滌後，加鹽酸液...

HIVandotherpredictorsofserumß-caroteneandretinolinpregnancy:across-sectionalstudyinZimbabwe

...heirstores.Infantsofwell-nourishedmothersconsumebreastmilkcontaining2µmolvitaminA/L,allowingvitaminAstorestoaccumulatefrom5µmolatdeliveryto100µmolattheendofthefirstyear(6).Incontrast,becausethebreastmilkofvitaminA–deficientmotherscontainsonly1µmol/L,thechildrenofthesemothers...

DNA探針標記常用試劑的配製

（1）10×缺口平移緩衝液：200mmol/lTris-HCl,pH7.4含50mmol/LMgCl2;100mmol/Lβ-巰基乙醇、1mg/mlBSA。　　（2）缺口平移反應終止液：200mmol/LNaCl;10mmol/lTris-HCl,pH7.4;11mmol/LEDTA;0.5%SDS。　　（3）DNaseⅠ：乾粉狀DNaseⅠ(2000～3000u/mg)溶於20mmol/lTris-HCl,pH...

甲巰咪唑對體外培養人甲狀腺細胞增殖的影響

...天，之後分別培養在5H和6H液中，不同濃度MMI（1、10、100μmol/L）狀態下孵育2天。以競爭性蛋白結合法測定cAMP。採用四氮唑藍比色法測定甲狀腺細胞增殖。結果MMI在較大範圍（1～100μmol/L）內不影響細胞內的第二信使cAMP的含量。...