伯氏疏螺旋體與嗜吞噬細胞無漿體感染抑制Th1型細胞因子產生
...rferiandAnaplasmaphagocytophilumoninhibitionofproductionofTh1cytokinesinthemurinehost. BAOFu-kai,ErolFikrig. (DepartmentofMicrobiologyandImmunology,KunmingMedicalCollege,Kunming650031,Yunnan,P.R.China;DepartmentofInternalMedicine,YaleUniversitySchoolofMedicine,NewHaven,CT06511,USA) Ab...
醫源資料庫;在線期刊;中國熱帶醫學雜誌;2007年第7卷第7期乙型腦炎病毒TaqMan逆轉錄聚合酶鏈反應檢測方法的建立
...halitisvirus. HUANGFu-xin,GAOShi-tong,ZHANGRen-li,etal. (ShenzhenMunicipalCenterforDiseaseControlandPrevention,Shenzhen518020,Guangdong,P.R.China) Abstract:ObjectiveToestablishaspecificandsensitivemethodfordetectingJapaneseencephalitisvirus(JEV)nucleicacidwithTaqManreversetrascripti...
醫源資料庫;在線期刊;中國熱帶醫學雜誌;2008年第8卷第9期PAI-14G/5G基因多態性與嚴重急性呼吸綜合徵後骨壞死△
...dfrombothgroupsPlasminogenactivatorinhibitor(PAI)bymeansofevzymelinkedimmunosorbentassay(ELISA),andPAI-14G/5GpolymorphismwasdetectedbypolymerasechainreactionPCRandSolidphaseoligonucleotideassay(SPOLA)[Result]Thebloodagentsofpost-SARSpatientschangedobviouslywithPAI-1polymorphismdetectedinpo...
醫源資料庫;在線期刊;中國矯形外科雜誌;2008年第16卷第9期克氏錐蟲的新過氧化物酶基因克隆表達研究
...acutestageofthediseaseandisunsatisfactoryduetotoxicityandlimitedefficacy.Somuchresearcheffortisdirectedatthedevelopmentofnewchemotherapeuticagents. T.cruziisexposedreactiveoxygenspexies(POS)throughoutitslifecycle.ROSssuchasthesuperoxideanion(O-2.)andhydrogenperoxide(H2O2)causecellulardamagedire...
醫源資料庫;在線期刊;中國熱帶醫學雜誌;2007年第7卷第6期乙型肝炎疫苗治療HBsAg陽性攜帶者新進展
...體,清除HBV。 用法:乙肝重組疫苗,兒童首次用量60μg,成人用量120μg,每次可增加60μg,最高量均可達180μg,局部常規消毒後,分別將疫苗注射在雙側足三裏及曲池穴位,陽陵泉和足三裏可交替使用,1次/周。注意有酸...
醫源資料庫;在線期刊;中國民康醫學;2008年第20卷第4期腎癌患者外周血中survivinmRNA的表達
...司OneStepRNAPCRKit(AMV)試劑盒說明進行RTPCR。總反應體系50μL:10×OneStepRNAPCRBuffer5μL,MgCl2(25mmol/L)10μL,dNTPMixture(10mmol/L)5μL,RnaseInhibitor(40u/μL)1μL,AMVRtaseXL(5u/μL)1μL,AMVOptimizedTaq(5u/μL)1μL,上游和下游引物各1&m...
醫源資料庫;在線期刊;現代泌尿外科雜誌;2008年第12卷第1期基於三維CT像重建研製納米仿生骨基質支架的研究
...ierforbonetissueengineering,poreratio88.61%±0.43%rangingfrom200-350μmofscaffolashowedmorestabledegradationspeedandexcellentaffinitysurface.[Conclusion]Imagingaidedfabricationtechniquehasagoodmatchbetweenhostandscaffold,showingvastresearchpotential. Keywords:iamging;threedimensio...
醫源資料庫;在線期刊;中國矯形外科雜誌;2009年第17卷第20期家族性肌萎縮硬化症酵母研究模型的探索
...niversity,Yantai264003【Abstract】ObjectiveToconstructtheSOD1genedeletionmutantofSaccharomycescerevisiaeandlayafoundationforstudyingthepathogenesisandtreatmentoffamilialamyotrophiclateralsclerosisbasedontheyeastmodel.MethodsTheSOD1:KanMX4genedeletionfragmentwasconstructedbylongflankinghomologyLFH...
醫源資料庫;在線期刊;濱州醫學院學報;2009年第32卷第2期MethodsfortheMeasurementofaBacterialEnzymeActivityinCell
...tsBrendanP.Burns1*,GeorgeL.Mendz2,StuartL.Hazell11SchoolofMicrobiologyandImmunologyand2SchoolofBiochemistryandMolecularGenetics,TheUniversityofNewSouthWales,Sydney2052,Australia.*Towhomcorrespondenceshouldbeaddressed.E-mail:B.Burns@unsw.edu.auABSTRACTThekineticcharacteristicsandregulationofaspartate...
藥品天地;專業藥學;實驗技術;生化實驗;酶學實驗SYBRGreenI熒光PCR檢測登革熱病毒基因方法的建立
...ofShenzhenEntryandExitInspectionandQuarantineBureau,Shenzhen518033;ShenzhenMunicipalAcademyofInspectionandQuarantine,Shenzhen518033,Guangdong,P.R.China) Abstract:ObjectiveTodevelopareal-timequantitativeRT-PCRmethodwithSYBRGreenItodetectdenguevirus.MethodsTheoptimalconditionsandsystemofrea...
醫源資料庫;在線期刊;中國熱帶醫學雜誌;2007年第7卷第10期