Thelatency-associatednuclearantigenofKaposisarcoma–associatedherpesvirusinducesBcellhyperplasiaandlymphoma
...onintheLANAtransgenicmice,weperformedimmunohistochemistry.Peanutagglutinin(PNA)andGL-7areassociatedwithactivatedGCBcells.IgDisamarkerformatureBcellsthatislostupondifferentiationlateduringGCmaturation.PNAstainingrevealedmarkeddifferencesbetweennormalandtransgenicanimals.AllLANAtransgenicmice(100%)pre...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第3期燒傷後急性腎功能衰竭
...全的性質和程度,其計算公式爲:注:UNa尿鈉(mmol);Pna血鈉(mmol);Cr肌酐清除率;PCr血肌酐(mg/dl);V尿量(L);GFR腎小球濾過率;UCr尿肌酐(mg/dl);FENa正常值1~3。FENa<1提示腎功能不全爲腎前性或功能性的。FENa>3提...
求醫問藥;疾病手冊;燒傷外科孕婦外周血胎兒有核紅細胞在產前診斷中的作用
...DQ序列特異性地確定爲胎源性[13]。LarsenRD等以肽核酸(PNA)探針進行熒光原位雜交(FISH)檢測胎兒有核紅細胞中的γ珠蛋白mRNA。並用Y染色體特異性PNA-FISH證實被檢測的γ珠蛋白mRNA是來源於胎兒NRˉBC[14]。 3孕婦外周血中...
醫源資料庫;在線期刊;中華現代婦產科學雜誌;2004年第1卷第2期Caspase-dependentand-independentpathwaysforcadmium-inducedapoptosisinculturedkidneyproximaltubulecells
...remeasuredthroughcleavageofacolorlesssubstratespecificforcaspase-3(Ac-DEVD-pNA)orcaspase-9(Ac-LEHD-pNA)releasingthechromophore,p-nitroaniline(pNA).Assayswerecarriedoutaccordingtomanufacturer‘sinstructionswithmodifications.Cells(5x106)werelysedfor15minonice,withmixingevery5min,andcytosolicextractsw...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第10期Protectionagainstcisplatin-inducednephrotoxicitybyacarbonmonoxide-releasingmolecule
...roteins)wasusedtomeasurecaspase-3activity.Thecolorimetricsubstrate(Ac-DEVD-pNA)waslabeledwiththechromophorep-nitroaniline(pNA),whichisreleasedfromthesubstrateoncleavagebyDEVDase(caspase-3)andproducesayellowcolorthatismonitoredspectrophotometricallyat405nm.Caspase-3activitywasexpressedaspicomolespNAl...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第4期Plasmacarotenoids,retinol,andtocopherolsandtheriskofprostatecancerintheEuropeanProspectiveInvestigationintoCancerandNutritionstudy
...ncerResearchUKEpidemiologyUnit,UniversityofOxford,Oxford,UnitedKingdom(TJK,PNA,NEA,RCT,andAWR);theNutritionandHormonesGroup(MJ,NS,PF,andSRinaldi)andthePathologyGroup(LE),InternationalAgencyforResearchonCancer,Lyon,France;theInstituteofCancerEpidemiology,DanishCancerSociety,Copenhagen,Denmark(ATj&osl...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2007年86卷第3期1-IntegrinsintheprimaryciliumofMDCKcellspotentiatefibronectin-inducedCa2+signaling
...or5minwith10mg/lfluorescein,rhodamine-conjugatedSNA,and/orpeanutagglutinin(PNA)andthenwashedbeforefixation,asdescribedbelow.Forantibodystaining,cellswerefixedfor15minin2.5%formaldehydeatroomtemperature.Next,thecellswerewashedtwotimesandpermeabilizedwith0.3%TritonX-100PBScontainingbovineserum(15g/l)f...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第11期DirectIdentificationofMajorBloodCulturePathogens,IncludingPseudomonasaeruginosaandEscherichiacoli,byaPanelofFluorescenceInSituHybridizatio
...dforsubculture.Fluorescenceinsituhybridization(FISH)withpeptidenucleicacid(PNA)probes(AdvanDxInc.,Woburn,Mass.)isanewFISHtechniqueusingfluorescein-labeledPNAprobestargetingrRNAforrapidandaccurateidentificationofmajorBCpathogens(1,4,5,9).Inthisnoninterventionalcohortstudy,PNAprobesforPseudomonasaerug...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第4期Urokinase-TypePlasminogenActivator,anEndogenousAntibiotic
...lasminogentoplasmin,whichhydrolyzesitschromogenicsubstrate(H-D-Val-Leu-Lys-pNA/2HCl;S-2251)toformfreepnitroanilin(pNA).TheformationoffreepNAisproportionaltothelevelofuPAenzymaticactivity.AllproductswerethenincubatedwithS.aureusLS-1for6h.ThebactericidaleffectsofuPAandinactivateduPAwereanalyzedinaccor...
醫源資料庫;在線期刊;傳染病學雜誌;2005年第191卷第15期ComplementreceptorsregulatedifferentiationofbonemarrowplasmacellprecursorsexpressingtranscriptionfactorsBlimp-1andXBP-1
...lliculardendriticcell;GC,germinalcenter;NP,(4-hydroxy-3-nitrophenyl)acetyl;PNA,peanutagglutinin.Protectiveimmunologicalmemoryagainstreinfectionwithmostviruseslargelydependsontheinductionoflong-lastingantibodyresponses.Thisconceptprovidesthebasisofallsuccessfulvaccinesusedtodate(1).Bcellmemoryischara...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第6期