ExpressionandfunctionofCOXisoformsinrenalmedulla:evidenceforregulationofsaltsensitivityandbloodpressure
...5‘-(FAM)-TGCAGCCATTTCCTTCTCTCCTGTAAGTTCT-(TAMARA)fortheprobe(accessionno.BC052900).Primer-probemixturesfor18SrRNAandCOX-1werepurchasedfromAppliedBiosystems(catalognos.4310893EandMm00477214_A1,respectively).Real-timePCRamplificationwasperformedusingtheTaqManUniversalPCRMasterMixandthePrism7900Seque...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第3期腦缺血後神經元程序性死亡與遲發性神經元壞死及相關基因的研究進展
...酸化修飾等途徑誘導神經細胞c-fos表達[31~34]。 4.2bc1-2基因家族bcl-2是從小鼠B細胞淋巴瘤中分離得到的原癌基因,編碼26kD的胞漿膜蛋白。bcl-2家族是目前研究得較爲深入的與PCD密切相關的基因,包括bcl-2,bcl-Xl,bcl-Xs和bax。現...
合作平臺;在線期刊;中華現代臨牀醫學雜誌;2005年第3卷第16期DirectInvolvementofBreastTumorFibroblastsintheModulationofTamoxifenSensitivity
...parationswereassessedinhomotypicfibroblastcultureswithanti-Snail1antibody(AbcamInc.,Cambridge,MA).Ineachcase,negativecontrolswereoverlaidwiththeappropriatemouseorrabbitIgGisotype.Theslideswereoverlaidwithavidin-biotin-conjugatedgoatanti-mouseoranti-rabbitIgGanddevelopedwithVectastainABCkit(VectorLab...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第5期LackofEndothelialNitric-OxideSynthaseLeadstoProgressiveFocalRenalInjury
...ubules.ImmunohistochemicallocalizationofeNOSwasperformedwiththeuseofeitherAbcam??santibodyab5589orSantaCruzantibodysc-8311,bothatdilutionsof1:100,withsubsequentdevelopmentintheDAKOLSAB2systemfollowedbydiaminobenzidine(DAB)(DAKOCorporation,Carpenteria,CA).Additionalantibodiesusedincludedplatelet-endo...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第1期Counter-RegulatoryFunctionofProteinTyrosinePhosphatase1BinPlatelet-DerivedGrowthFactor-orFibroblastGrowthFactor-InducedMotilityandProliferati
...incubationwithanti-PCNAatadilutionof1:200.DetectionwascarriedoutbyusingtheABC(Avidin-BiotinComplex)methodwithDAB(3,3‘-diaminobenzidine)assubstrate.TotalandPCNA-positivecellnumbersweredeterminedfromanalysisoffourmicroscopicfieldsfromeachofthetissuesections,atmagnificationofx400.Thefractionofprolife...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2006年第26卷第3期DeterminantsoflowbirthweightamongHIV-infectedpregnantwomeninTanzania
...CScountsystem(BectonDickinson,SanJose,CA).HemoglobinwasmeasuredwitheitheraCBC5CoulterCounter(CoulterCorporation,Miami)orbythecyanmethemoglobinmethodwithuseofacolorimeter(CorningInc,Corning,NY).SerumretinolconcentrationsweremeasuredbyHPLC.Serawerealsotestedforthepresenceofsyphilisantibodiesbyusingthe...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2001年74卷第6期RegulationofTissueFactor–MediatedInitiationoftheCoagulationCascadebyCellSurfaceGrp78
...nmol/LwasincubatedwithvaryingamountsofrecombinantGrp78(Stressgen,Vancouver,BC)inthepresence,orabsenceofanti-Grp78Gfor4hoursat22°CinHBSAbuffer(20mmol/LHepes,150nmol/LNaCl,0.5%bovineserumalbumin;pH7.4)with5mmol/LCaCl2.VIIa(25nmol/L)andX(25nmol/L)werethenaddedtostartthereaction.After10minutesat37°C,t...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第8期IsolationandCharacterizationofVasohibin-2asaHomologueofVEGF-InducibleEndothelium-DerivedAngiogenesisInhibitorVasohibin
...elimpethemocyaninconjugateasdescribedpreviously.3TheresultantmAb5E3(IgG1,subclass)waspurifiedbyproteinA-agaroseaffinitychromatography(MAPSIIkit,Bio-RadLaboratoriesInc,Hercules,Calif).Anti-mousevasohibin-1and-2polyclonalantibodies(Abs)werepreparedusingCys-mousevasohibin-1peptide(Gly296-Arg309)orCys-m...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2006年第26卷第5期Renalischemiainducestropomyosindissociation-destabilizingmicrovillimicrofilaments
...beledpelletswereresuspendedinPBSandmountedinaglycerol-PBSmediumcontainingDABCO.Tropomyosinisoformidentification.Withtheuseofisoform-specificantibodies,multipleisoformsoftropomyosinwereidentifiedinratrenalcorticalhomogenates.Kidneysfromuntreatedratswereperfuse-flushedwithPBS,removed,anddecapsulated.T...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第5期Adenovirus-MediatedExpressionofTissueFactorPathwayInhibitor-2InhibitsEndothelialCellMigrationandAngiogenesis
...tsThicksectionsorwhole-mountpreparationsofspongesharvested2and3weeksaftersubcutaneousimplantationwereexaminedbyconfocalmicroscopy24,25afterimmunostainingforvariousmarkers.DistributionofGFP,usedasanindicatorofinfectionefficiency,wasquasi-uniformamongthedifferentlyinfectedsponges(supplementalFigureIIa...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2007年第27卷第2期