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Disease-associatedmutationsaffectintracellulartrafficandparacellularMg2+transportfunctionofClaudin-16
...,proteinsynthesisanddegradationwereinhibitedwithcycloheximideandN-acetyl–leu-leu-norleucinal(ALLN),respectively,andcellswereshiftedto37°CpriortostainingforGM130(Figure5,BandE)orCD63(Figure5,CandF).ThisexperimentdetermineswhetheraparticularCldn16mutantisretainedintheGolgicomplexorexitstheGolgicomp...
醫源資料庫；在線期刊；臨牀研究雜誌；2006年第116卷第4期 -
AcuteinvivoelevationofintravasculartriacylglycerollipolysisimpairsperipheralTcellactivationinhumans
....Oncethesepathwaysareactivated,triggered-antigen-specificTcellsproduceinterleukin(IL)2andcommittoproliferation.n–3Polyunsaturatedfattyacids(PUFAs)haveimmunosuppressiveeffectsonTcellfunctionsinmice(7).EarlyeventsofTCRsignaling,calciummetabolism,andIL-2secretionareimpairedinJurkatTcellsintheprese...
醫源資料庫；在線期刊；美國臨牀營養學雜誌；2005年82卷第5期 -
InSilicoPredictionofPregnaneXReceptorActivatorsbyMachineLearningApproache
...pingdoorallowingligandstoenterthecentraloftheligand-bindingsite.Inaddition,Leu209locatedneartheCterminusof2shiftedinpositionbyupto7.7Ewhenboundbydifferentligands(Watkinsetal.,2003b).Bindingbycoactivatorsfurtherstabilizestheboundorientationsofligands.Takentogether,thelargeandflexibleligand-bindingpoc...
醫源資料庫；在線期刊；分子藥理學雜誌；2007年第69卷第1期 -
PioglitazoneStimulatesApolipoproteinA-IProductionWithoutAffectingHDLRemovalinHepG2Cells
...ulturematerials,media,andFBSwereobtainedfromSigmaChemicalCompany.L-[4,5-3H]Leucineand[3H]cholesterolwerepurchasedfromAmershamCorporation.HepG2cellsandhumanmacrophageTHP-1celllinewereobtainedfromAmericanTypeCultureCollection.ThepolyclonalantibodiesforhumanapoA-IandapoA-IIwerepurchasedfromBoehringerMa...
醫源資料庫；在線期刊；動脈硬化血栓血管生物學雜誌；2007年第27卷第11期 -
六、趨化因子
...（classical)白細胞趨化物質主要有補體片段C5a、白三烯B4（leukotrininB4,LTB4)、血小板激活因子（platelet-activatingfactor,PAF)和fMLP（N-formylmethionyl-leucyl-phenyl-alanine,N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸），這些趨化物質的受體同屬於G蛋白偶聯...
醫源資料庫；醫源圖書館；教材類；細胞和分子免疫學 -
Specificandnonspecificimmuneresponsestofastingandrefeedingdifferinhealthyyoungadultandelderlypersons
...ntibodies(Dako,Trappes,France).AssessmentofbloodcellularityThetotalnumberofleukocytes,leukocytedifferentialcount,redbloodcellnumber,hemoglobin,hematocrit,andplateletsweremeasuredwithuseofaCoultercounter(Coultronics,Margency,France).IndexesrelatedtospecificimmunestatusLymphocytesubpopulationsweremeas...
醫源資料庫；在線期刊；美國臨牀營養學雜誌；2001年74卷第5期 -
LysophosphatidylcholineEnhancesCytokineProductionofEndothelialCellsviaInductionofL-TypeAminoAcidTransporter1andCellSurfaceAntigen4F2
...dmembraneproteinlevelsofLAT1confirmedbyWesternblotanalysisandalsouptakeofL-leucine,whichwasinhibitedbyacompetitiveinhibitorforLAT1.Thereleaseofinterleukin6(IL-6)andIL-8wasincreasedinLysoPC-treatedcellsandwasattenuatedbytheLAT1inhibitor.Conclusions—Thesefindingssuggestthatanincreaseinuptakeofneutra...
醫源資料庫；在線期刊；動脈硬化血栓血管生物學雜誌；2004年第24卷第9期 -
IsolationandCharacterizationofVasohibin-2asaHomologueofVEGF-InducibleEndothelium-DerivedAngiogenesisInhibitorVasohibin
...ingCys-mousevasohibin-1peptide(Gly296-Arg309)orCys-mousevasohibin-2peptide(Leu230-Arg242)conjugatedwithmaleimide-activatedkeyholelimpethemocyanin.Rabbitswereimmunizedsubcutaneouslywithmousevasohibin-1ormousevasohibin-2peptideconjugates.Thefirstinjectionconsistedof200µgofconjugateinanequalvolum...
醫源資料庫；在線期刊；動脈硬化血栓血管生物學雜誌；2006年第26卷第5期 -
R-Cadherin:?-CateninComplexandItsAssociationWithVascularSmoothMuscleCellProliferation
...1.0-foldand4.1±0.98-fold,andincreasedthenumberofcellswith?-catenininthenucleusandexpressingcyclinD1inaorticrings.Conclusions—TheseresultssuggestthatR-cadherinexpressionand?-cateninsignalingmaybeassociatedwithincreasedcyclinD1expressionandVSMCproliferationandmaythereforeplayanimportantroleinvascul...
醫源資料庫；在線期刊；動脈硬化血栓血管生物學雜誌；2004年第24卷第7期 -
EffectofhydrogenperoxideonROMKchannelsinthecorticalcollectingduct
...0µg/mlphenylmethylsulfonylfluoride,5µg/mlaprotinin,5µg/mlleupeptin,and2µg/mlpepstatin].ForactivityassayofPTK,10µlofthesamplewereincubatedinatotalvolumeof30µlcontaining200µM[32P]ATP(1cpm/fmol),12mMmagnesiumacetate,2mMMnCl2,0.3mMdithiothreitol,0.5mMsodiumortho...
醫源資料庫；在線期刊；美國生理學雜誌；2007年第290卷第4期