燒傷後急性腎功能衰竭
...全的性質和程度,其計算公式爲:注:UNa尿鈉(mmol);Pna血鈉(mmol);Cr肌酐清除率;PCr血肌酐(mg/dl);V尿量(L);GFR腎小球濾過率;UCr尿肌酐(mg/dl);FENa正常值1~3。FENa<1提示腎功能不全爲腎前性或功能性的。FENa>3提...
求醫問藥;疾病大全;腰部NeutralizationofPlasminogenActivatorInhibitorI(PAI-1)bytheSyntheticAntagonistPAI-749viaaDualMechanismofAction
...eenwich,CT).HEPES-freeacid,aprotinin,p-aminobenzamidine(PAB),D-Val-Leu-Lys-pNA(plasminsubstrate)andSAR-Pro-Arg-pNA(trypsinsubstrate)werefromSigma-Aldrich(St.Louis,MO).PEG-8000wasfromU.S.BiochemicalCorp.(Cleveland,OH).Dimethyldecyl-phosphineoxide(apo-10)wasfromCalbiochem(SanDiego,CA).Human1-antitryps...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第10期IdentificationofanovelsignalinthecytoplasmictailoftheNa+:HCO3-cotransporterNBC1thatmediatesbasolateraltargeting
...n-tetramethylrhodamine,asamarkeroftheactincytoskeleton,andpeanutagglutinin(PNA)-lectinAlexaFluor568conjugate,asamarkerofextracellularglycans,whichareexpressedontheapicalmembrane.ThepolarizedMDCKcellstransientlyexpressingepitope-taggedwild-typeNBC1ormutantNBC1(F1013A,F1013L,andL1014A)werelabeledwitha...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第4期Actincytoskeletonregulatesextracellularmatrix-dependentsurvivalsignalsinglomerularepithelialcells
...-3,-8,and-9activitiesweremeasuredusingacolorimetricassaywithp-nitroaniline(pNA)-labeledsubstrates,asdescribedpreviously(7).Adherentcellswerelysedinbuffercontaining50mMHEPES,100mMNaCl,0.1%CHAPS,1mMDTT,100μMEDTA,pH7.4(4°C).Themixturewascentrifugedat14,000gfor10min.Supernatantswerecollectedandadjuste...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第12期你知道這些遺傳物質嗎?
...體系。在地球生命起源前的條件下,還容易構築起冗長的PNA分子,即使在100℃的溫度下也不例外。(二醇核酸),結構甚至比TNA還要簡單,鏈中只有三個碳原子,卻依然能形成螺旋型分子,同DNA頗爲相似。(澱粉核酸),由附着...
行業資訊;臨牀快報;遺傳與基因組Bcl-2–relatedproteinA1isanendogenousandcytokine-stimulatedmediatorofcytoprotectioninhyperoxicacutelunginjury
...batedwiththecolorimetricsubstrateAc-Asp-Glu-Val-Asp-p-nitroanilide(Ac-DEVD-pNA),N-acetyl-Ile-Glu-Thr-Asp-p-nitroanilide(Ac-IETD-pNA),orAc-Leu-Glu-His-Asp-p-nitroanilide(Ac-LEHD-pNA)forcaspases-3,-8,and-9,respectively.Thereleaseofp-nitroanilidefromAc-DEVD-pNA,Ac-IETD-pNA,orAc-LEHD-pNAwasmeasuredat405...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第4期第四節 免疫細胞化學技術的某些新進展
...tributionofbindingsitesforDolichosbiflrousagglutininandforpeanutagglutinin(PNA)inmouseorgantissues.JHistochemCytochem,1981;29:779 12.王益壽.葡萄球菌蛋白A的性質和應用.國外醫學(生物製品分冊),1980;4:145 13.張華忠,等.凝集素在人體正常組...
醫源資料庫;醫源圖書館;教材類;實用免疫細胞與核酸ActivationoftheMelastatin-RelatedCationChannelTRPM3byD-erythro-Sphingosine
...potentialsduringsubsequentapplicationofNa+andCa2+,arelativepermeabilityPCa/PNa=1.91±0.51(n=5)wascalculated.Theionconcentrationswerecorrectedfortherespectiveactivitycoefficients(0.76for140mMNaCl,0.517for100mMCaCl2).EffectsofSPHafterStoreDepletion,InhibitionofInositol1,4,5-Trisphosphate(IP3)Receptors...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第3期ZIP8,MemberoftheSolute-Carrier-39(SLC39)Metal-TransporterFamily:CharacterizationofTransporterProperties
...sfectedwithGFP-NBC1werecostainedwithanapicalmembranemarkerpeanutagglutinin(PNA)-lectin(Invitrogen)at1/200dilutionfor1hatroomtemperatureandthenmountedforconfocalanalysis.ImagesweretakenonaZeissLSM510confocalmicroscope(CarlZeiss,Oberkochen,Germany).BothZ-lineandZ-stackimageswereobtained,usingtheLSM5Im...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第7期Claudins6,9,and13aredevelopmentallyexpressedrenaltightjunctionproteins
...changeintheparacellularpassivefluxofions.NeonatalproximaltubuleshaveahigherPNa/PClratioandlowerchlorideandbicarbonatepermeabilitiesthanadultproximaltubules.Claudinsarealargefamilyofproteinswhicharethegatekeepersoftheparacellularpathway,andclaudinisoformexpressiondeterminesthepermeabilitycharacterist...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第12期