OverexpressionofarginaseIinenterocytesoftransgenicmiceelicitsaselectiveargininedeficiencyandaffectsskin,muscle,andlymphoiddevelopment
...intheF/A-2transgenicsexceedstheMichaelisconstantofarginyltransferRNA(tRNA)synthetase(EC6.1.1.19)forarginine(20µmol/L;49);thus,incompletechargingofarginyl-tRNAasthelimitingfactorforproteinsynthesisappearslesslikely.However,amoreimportantargumentthancellulararginineconcentrationisthatincomp...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2002年76卷第1期第二節 衰老機理研究進展
...-10個mtDNA分子,mtDNA基因組含有編碼兩種rRNA(12S和16S)、22種tRNA及細胞氧化磷酸化有關的13條多肽鏈(細胞色素B和C、氧化酶I、Ⅱ、Ⅲ亞單位、ATP3亞單位6和8兩部分以及呼吸鏈NADH脫氫酶的7個亞單位即ND1-ND6以及ND4L)的原因。 MtDNA...
醫源資料庫;醫源圖書館;教材類;老年醫學第五節 某些肝病的生化機制
...照組爲低,C-亮氨酸向腦核蛋白的摻入減少,腦內總RNA及tRNA減少。這表明FAS動物腦內蛋白質合成的抑制可解釋人類FAS中智能發育延遲的現象。長期大量飲酒不僅危害人類的健康,還影響下一代的發育成長。 二、肝硬化的生化...
醫源資料庫;醫源圖書館;教材類;臨牀生物化學TristetraprolinRegulatestheExpressionoftheHumanInducibleNitric-OxideSynthaseGene
...proteinexpression,whereashumaniNOSpromoteractivitywasnotaffected.AnimportantRNAbindingproteinregulatedbythep38MAPKpathwayandinvolvedintheregulationofthestabilityofseveralmRNAsistristetraprolin.RNaseprotection,quantitativereal-timepolymerasechainreaction,andWesternblotexperimentsshowedthatcytokinesus...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第6期RoleforMacrophageMetalloelastaseinGlomerularBasementMembraneDamageAssociatedwithAlportSyndrome
...eionizedformamide,2xstandardsalinecitrate,10Tween20,and1mgofEscherichiacolitRNA.Thehybridizationsolutionconsistedof50ngheat-denaturedriboprobe,50%deionizedformamide,8%dextransulfate,10%Tween20,2xstandardsalinecitrate,20%tRNA,and10mg/mlboiledsalmonsperm.Slideswerehybridizedat45??Covernight.TheDIGWash...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第7期實驗室常用技術參數資料
...s;10618SrRNA19006.1×10519SrRNA17005.5×1055SrRNA1203.6×104tRNA(大腸桿菌)752.5×1043.常用核酸蛋白換算數據 (1)重量換算 1μg=10-6g 1pg=10-12g 1ng=10-9g 1fg=10-15g (2)分光光度換算: 1A260...
藥品天地;專業藥學;實驗技術;基本實驗技術;實驗數據Ornithinemetabolisminmaleandfemaleratkidney:mitochondrialexpressionofornithineaminotransferaseandarginaseII
...ormamide,10%dextransulfate,1xDenhardt‘ssolution,0.5mg/mlyeasttransferRNA(tRNA),and8ngeithersense-orantisense-labeledriboprobes.Theincubatingmediumcontained3.106cpm·(50µlperslide)-1.Posthybridizationanddetection.Thesectionswererinsedin1xSSCcontaining50%formamidefor30minat55°C,2xSSC...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第4期High-dosevitamintherapystimulatesvariantenzymeswithdecreasedcoenzymebindingaffinity(increasedKm):relevancetogeneticdiseaseandpolymorphisms
...nassociatedmyopathy,anda3250TCmutationinthegeneformitochondrialtransferRNA(tRNA)leucine(UUR)(seeOMIM590050;alsodiscussedinthesectiononniacin)hadasustainedclinicalresponsetoriboflavin(50mg/d)(219).Theauthorsrefertoreportsofriboflavinbeingeffectivein11patientswithcomplexIdeficiency,althoughtheunderlyi...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2002年75卷第4期