InhibitionofVascularEndothelialGrowthFactorCotranslationalTranslocationbytheCyclopeptolideCAM741
...-link;,nonprocessedNCs;,additionalunspecifiedcross-links;,residualpeptidyl-tRNA-NCs.B,schematicrepresentationoftheconstructsused.Invitrotargetingandchemicalcross-linkingwithBMHoftruncatedVEGFSPmutantsfusedto54aminoacidresiduesSEAPmaturedomaincontainingacysteineatposition30(V30C;fourthpositionoftheSE...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第6期H2-M3–restrictedCD8+TcellsarenotrequiredforMHCclassIb–restrictedimmunityagainstListeriamonocytogenes
...ges;fmt,formyltransferase;ICCS,intracellularcytokinestaining;MTF,methionyl-tRNAfMetformyltransferase;PDF,peptidedeformlyase.Althoughinnateimmuneresponsesareessentialtolimitthespreadofintracellularbacterialinfections,itistheadaptiveimmuneresponsethatallowsthebodytocompletelycleartremendousbacterialbu...
醫源資料庫;在線期刊;實驗醫學雜誌;2006年第203卷第2期PostlipopolysaccharideOxidativeDamageofMitochondrialDNA
...ributestodiseasesofdefectivemtDNA(11,14,15).Themitochondrialgenomeencodes22tRNAs,2rRNAs,and13polypeptidesoftheelectrontransportcomplexes.Thus,mostoftheapproximately200mitochondrialproteinsareencodedinthenucleus.Nucleargenesofrespirationrespondrapidlytocellactivationbytheinductionofspecifictranscript...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2003年第167卷第4期InvitrocharacterizationofaldosteroneandcAMPeffectsinmousedistalconvolutedtubulecells
...RTreagentswerecheckedforampliconcontaminationbytheinclusionofacontrolwithoutRNA.DifferentPCRwereperformedusingspecificprimers(summarizedinTable1)andconductedin25µlcontaining20mMTris·HCl(pH8.4),50mMKCl,1.5mMMgCl2,200µMdNTPs,0.8µMofeachprimer,1UTaqpolymerase(BoehringerMannheim...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第5期OverexpressionduringChronicInjuryandInductionofProinflammatoryActionsinHepaticStellateCells
...ressionbyRNaseprotectionassay,asdescribedinMaterialsandMethods.TransferRNA(tRNA)andperipheralbloodmononuclearcellRNA(PBMC)wereusedasnegativeandpositivecontrolsforhybridization,respectively.C:HSCswereincubatedinthepresenceorabsenceof500ng/mlresistinfor48hours.Aliquotsofcellsupernatantwereanalyzedinch...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第12期PeroxiredoxinIsaPotentCytoprotectiveEnzymeintheEpidermis
...erentlinesandfromwild-typeanimalswasanalyzedforthepresenceofPrdx6mRNAbyRPA.tRNA(20µg)wasusedasanegativecontrol.Thehybridizationprobe(1000cpm)wasloadedinthelanelabeled“probe“andusedasasizemarker.OneµgofeachRNAsamplewasloadedona1%agarosegelandstainedwithethidiumbromide.Thebandcorrespon...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第10期Heme-regulatedeIF2kinasemodifiesthephenotypicseverityofmurinemodelsoferythropoieticprotoporphyriaand?-thalassemia
...starvationthroughtheC-terminaldomain,whichcontainsahistidyltransferRNA(His-tRNA)synthase–likesequence(reviewedinrefs.19).PERKishighlyexpressedinsecretorytissues,particularlythepancreas,andisactivatedbyERstress.PERKcontainsalumenaldomainwhichissimilartothesensordomainoftheER-stresskinase,Irel(revie...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第6期第一節 原位雜交組織化學概述
...配方沉澱探針 7mol/L醋酸銨100μl 100%乙醇750μl tRNA(10mg/ml) 2μl 置於—20℃2h後回暖至室溫,在14000rpm離心30min。 (4)小心將乙醇輕輕傾出,待試管內乾燥後再用無菌蒸餾水稀釋到10ng/μl濃度。 (5...
醫源資料庫;醫源圖書館;教材類;實用免疫細胞與核酸MolecularDeterminantsoftheAgonistBindingDomainofaP2XReceptorChannel
...aritiesbetweentheLys180-Lys326ectodomainregionofP2X4andtheclassIIaminoacyl-tRNAsynthetasesasaguidetogenerateathree-dimensionalmodelofthereceptor-bindingsiteandtodesignmutants.Theinterplaybetweenhomologymodelingandsite-directedmutagenesissuggestedthatAsp280residueofP2X4RcoordinatesATPbindingviathemag...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第4期Geneticcausesofhumanheartfailure
...dcardiomyopathyandhearinglossassociatedwithanovelmutationinthemitochondrialtRNA(Lys)gene(G8363A).Am.J.Hum.Genet.58::933-939.Santorelli,F.M.etal.1999..Maternallyinheritedcardiomyopathy:anatypicalpresentationofthemtDNA12SrRNAgeneA1555Gmutation.Am.J.Hum.Genet.64::295-300.Arbustini,E.etal.1998..Mitochon...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第3期