TrappingofaNonpeptideLigandbytheExtracellularDomainsoftheGonadotropin-ReleasingHormoneReceptorResultsinInsurmountableAntagonism
...ndstructuralcomponentsrequiredforinsurmountableactivityofaGnRHRantagonist.TAK-013behavesasaninsurmountableantagonistatthehumanreceptor(hGnRHR)butasasurmountableantagonistatthemacaquereceptor(mGnRHR).MutationoftheeightresiduesthatdifferbetweenhGnRHRandmGnRHRidentifiedSer-203andLeu-300inextracellularl...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第8期StromallyExpressedc-JunRegulatesProliferationofProstateEpithelialCells
...stromallyproducedIGF-1up-regulatesepithelialmitogen-activatedproteinkinase,Akt,andcyclinDproteinlevelswhiledown-regulatingthecyclin-dependentkinaseinhibitorp27.Thesedatasuggestthatstromallyexpressedc-JunmaypromoteprostaticepithelialproliferationthroughIGF-1asaparacrinesignalthat,inturn,canpromotepro...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第10期UnexpectedprolongedpresentationofinfluenzaantigenspromotesCD4Tcellmemorygeneration
TrudeauInstituteInc.,SaranacLake,NY12983Thekineticsofpresentationofinfluenzavirus–derivedantigens(Ags),resultinginCD4Tcelleffectorandmemorygeneration,remainsundefined.Naiveinfluenza-specificCD4Tcellsweretransferredintomiceatvarioustimesafterinfluenzainfectiontodeterminethedurationandimpactofvirus-...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第202卷第5期EssentialRoleofC-RelinNitric-OxideSynthase-2TranscriptionalActivation:Time-DependentControlbySalicylate
...feron-(LPS/IFN)onC-RelDNAbindinginRAW264.7.LPS/IFN-stimulatedC-Relbindingpeakedat4to8handdeclinedat24h.TransfectionofcellswithaC-RelsmallinterferingRNAabrogatedC-Relbindingatalltimepoints.LPS/IFNproducedsuperoxideat4h,whichsubsidedat8h.C-RelbindingandNOS-2expressionwereabrogatedbysuperoxidedismutase...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第12期MaintainedENaCtraffickinginaldosterone-infusedratsduringmineralocorticoidandglucocorticoidreceptorblockade
...rationinthehighphysiologicalrange(3to4nM).AldosteroneinfusiondecreasedplasmaK+concentrationcomparedwithuntreatedcontrolrats.Cotreatmentwithspironolactonecompletelyblockedthealdosterone-induceddecreaseinplasmaK+.ImmunoblottingandimmunohistochemistryshowedincreasedproteinabundanceofNa-K-ATPase1-subuni...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第1期RasGRP1ConfersthePhorbolEster-SensitivePhenotypetoEL4LymphomaCells
...rfurtheranalysis.RasActivationAssay.Raf-1RBDagarose(UpstateBiotechnolXogy,LakePlacid,NY),whichspecificallybindstoGTP-boundRas,wasusedtopulldownactiveRas.Whole-cellextracts(1000µgin1ml)wereaddedto10µgofRaf-1RBDagarosefor1hat4°C.Precipitateswerewashedthreetimeswith1mlofice-coldlysisbuf...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第1期ImmunomodulatoryEffectofCombinationTherapywithLovastatinand-Aminoimidazole--Carboxamide--ß-D-RibofuranosideAlleviatesNeurodegenerationinExpe
...lacebo)orlovastatinandAICARincombinationoncedaily.Animalsweresacrificedonpeakclinicalday,day13afterimmunization,oronremission(25daysafterimmunization)tocollectserumandspinalcord(SC)tissues.AnimalsdevelopingsevereEAEdiseaseaftertreatmentwithdrugorplaceboweresacrificed14daysafterimmunizationonwardsasp...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第9期Insulin-LikeGrowthFactorType-IReceptor-DependentPhosphorylationofExtracellularSignal-RegulatedKinase1/2butnotAkt(ProteinKinaseB)CanBeInduce
...icropodophyllin(PPP)inhibitsphosphorylationofIGF-1Randphosphatidyl-3kinase/Akt(proteinkinaseB)signalingmoleculeswithoutinterferingwiththehighlyhomologousinsulinreceptor.Furthermore,PPPcausesregressionoftumorgraftsandsubstantiallyprolongsthesurvivalofanimalswithsystemictumordisease.Itisofinterestthat...
醫源資料庫;在線期刊;分子藥理學雜誌;2008年第70卷第3期ThePoxvirusProteinA52RTargetsToll-likeReceptorSignalingComplexestoSuppressHostDefense
...rviralRNA.A52Rassociateswithbothinterleukin1receptor–associatedkinase2(IRAK2)andtumornecrosisfactorreceptor–associatedfactor6(TRAF6),twokeyproteinsimportantinTLRsignaltransduction.Further,A52Rcoulddisruptsignalingcomplexescontainingtheseproteins.AvirusdeletionmutantlackingtheA52Rgenewasattenuate...
醫源資料庫;在線期刊;實驗醫學雜誌;2003年第197卷第3期ThePoxvirusProteinA52RTargetsToll-likeReceptorSignalingComplexestoSuppressHostDefense
...rviralRNA.A52Rassociateswithbothinterleukin1receptor–associatedkinase2(IRAK2)andtumornecrosisfactorreceptor–associatedfactor6(TRAF6),twokeyproteinsimportantinTLRsignaltransduction.Further,A52Rcoulddisruptsignalingcomplexescontainingtheseproteins.AvirusdeletionmutantlackingtheA52Rgenewasattenuate...
醫源資料庫;在線期刊;美國病理學雜誌;2003年第162卷第3期