OxidativeStressGeneExpressionandProteinChangesInducedintheHumanPlacentaduringLabor
...rfromsnap-frozenplacentaltissueorfromtissuecollectedinRNALater(Ambion,Inc.,Austin,TX)usingTRIreagent(Sigma).TheRNAwasquantifiedbyspectrophotometry(NanodropTechnologies,Wilmington,DE)andRNAintegrityassessedusinganAgilent2100Bioanalyzer(AgilentTechnologiesUKLtd.,Stockport,Cheshire,UK).Inbrief,20µ...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第10期Retinoid-InducedApoptosisinHL-60CellsIsAssociatedwithNucleolinDown-RegulationandDestabilizationofBcl-2mRNA
...Capped32P-labeledtranscriptswerepreparedusingamMessagemMachineT7kit(Ambion,Austin,TX)accordingtothemanufacturer‘sinstructions.Poly(A)tailsofapproximately150nucleotideswereaddedtothe3‘endsofthetranscriptsusingapoly(A)tailingkit(Ambion),andunincorporatednucleotideswereremovedbyG-25spincolumnchroma...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第1期IsolationofaNovelPopulationofMultipotentAdultStemCellsfromHumanHairFollicles
...aseChainReaction(RT-PCR)TotalRNAwasisolatedusingaCells-to-cDNAIIkit(Ambion,Austin,TX).Perkitprotocol,20ngoftotalRNAwasusedforthefirst-strandcDNAsynthesis.Real-timePCRwasperformedonaniCycler(Bio-Rad,Hercules,CA)using1µlofcDNAandiQSYBRGreenreagents(Bio-Rad).Standardreal-timePCRprotocolwasusedwit...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第6期IncreasedGlucocorticoidReceptorAltersSteroidResponseinGlucocorticoid-insensitiveAsthma
...A(siRNA,targetingGCR,annealed)wascustomdesignedandsynthesizedbyAmbion,Inc.(Austin,TX).ThesequencetargetinghumanGCRwasasfollows:sense,5‘-GGCUUUUCAUUAAAUGGGAtt-3‘;antisense,5‘-UCCCAUUUAAUGAAAAGCCtc-3‘.Tobeabletoestimatetheefficiencyoftransfection,siRNAwaslabeledwithaCy5siRNAlabelingkit(Ambion)...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2006年第173卷第6期Upregulationoflectin-likeoxidizedlow-densitylipoproteinreceptor-1(LOX-1)inendothelialcellsbynitricoxidedeficiency
...ntrolwasalsocoamplifiedbyincorporating18S/18ScompetitionmRNAprimer(Ambison,Austin,TX)inanotherreactiontube.Theappropriate18S:18ScompetimerratioandoptimalnumberofPCRcycleswereinaccordwiththemanufacturer‘srecommendations.Internalstandardprimersfor18Sgeneratedaproductof489bp.Productswereanalyzedbyrun...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第7期Glutamine:commerciallyessentialorconditionallyessential?Acriticalappraisalofthehumandata
...diation.JPENJParenterEnteralNutr1991;15:17S(abstr).WustemanM,TateH,WeaverL,AustinS,NeeleG,EliaM.Theeffectofenteralglutaminedeprivationandsupplementationonthestructureofratsmallintestinemucosaduringasystemicinjuryresponse.JPENJParenterEnteralNutr1995;19:227.SpaethG,GottwaldT,HaasW,HolmerM.Gluta...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2001年74卷第1期AdoptivelyTransferredAllergen-SpecificTCellsCauseMaternalTransmissionofAsthmaRisk
...erimmunoassaykitsfromLincoResearch(St.Charles,MI)andxMAPtechnology(Luminex,Austin,TX),accordingtothemanufacturer??sinstructions.Color-codedmicrosphereswereusedforthedetectionofmouseinterferon-(IFN-),interleukin(IL)-4,IL-5,IL-10,andIL-13.Standardsandinternalcontrolswereaddedforeachcytokine.Separateas...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第6期TransportofestronesulfatebythenovelorganicaniontransporterOat6(Slc22a20)
...omlinearizedOat6cDNAusingthemMessagemMachineinvitrotranscriptionkit(Ambion,Austin,TX).After3days,oocyteswererandomlydividedintoexperimentalgroups(n=5-10oocytes/group)andincubatedfor1hatroomtemperatureinoocyteRinger2(OR-2;inmM:82.5NaCl,2.5KCl,1Na2PO4,3NaOH,1CaCl2,1MgCl2,1pyruvicacid,and5HEPES,pH7.6)c...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第8期Stereochemicalassignment,antiinflammatoryproperties,andreceptorfortheomega-3lipidmediatorresolvinE1
...adylaminarshearstress.Proc.Natl.Acad.Sci.USA.93:10417–10422.Cheng,Y.,S.C.Austin,B.Rocca,B.H.Koller,T.M.Coffman,T.Grosser,J.A.Lawson,andG.A.FitzGerald.2002.RoleofprostacyclininthecardiovascularresponsetothromboxaneA2.Science.296:539–541.Serhan,C.N.2002.Endogenouschemicalmediatorsinanti-inflammati...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第5期SLC26A7:abasolateralCl-/HCO3-exchangerspecifictointercalatedcellsoftheoutermedullarycollectingduct
...BRL.BCECFwasfromMolecularProbes.AmMESSAGEmMACHINEKitwaspurchasedfromAmbion(Austin,TX).ThehumanmultipletissueblotswerepurchasedfromCLONTECH(PaloAlto,CA).Statisticalanalyses.Valuesareexpressedasmeans±SE.ThesignificanceofdifferencebetweenmeanvalueswasexaminedusingStudent‘st-testorANOVA.P<0.05w...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第1期