Caveolin-1FacilitatesMechanosensitiveProteinKinaseB(Akt)SignalingInVitroandInVivo
...mechanosensitiveactivationofphosphoinositide3-kinase(PI3-K)/proteinkinaseB(Akt)resultinginp27Kip1transcriptionaldownregulationandcellcycleentryofvascularsmoothmusclecells(VSMC).Inthisstudy,wefurtherelucidatedthesignalingfromoutside-intowardPI3-K/Aktinvitroandinaninvivomodelofelevatedtensileforce.Whe...
醫源資料庫;在線期刊;循環研究雜誌;2005年第95卷第4期TheInteractionofanImpermeantCationwiththeSheepCardiacRyRChannelAltersRyanoidAssociation
...ryanodinesiteonthechannel.TheseactionsinvolvebindingofTEA+todifferent,butweaklyinteracting,sitesintheRyRchannel.Theplantalkaloidryanodinebindswithhighaffinitytoaclassoflargehomotetrameric(monomer550kDa)intracellularmembraneCa2+-releasechannels,referredtoastheryanodinereceptor(RyR),andalterschannelfu...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第6期Calcium,vitaminD,milkconsumption,andhipfractures:aprospectivestudyamongpostmenopausalwomen
...ion.Design:Inan18-yprospectiveanalysisin72337postmenopausalwomen,dietaryintakeandnutritionalsupplementusewereassessedatbaselinein1980andupdatedseveraltimesduringfollow-up.Weidentified603incidenthipfracturesresultingfromlowormoderatetrauma.Relativerisks(RRs)fromproportionalhazardsmodelswerecontrolled...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2003年77卷第2期DifferentialActivationofMitogenicSignalingPathwaysinAorticSmoothMuscleCellsDeficientinSuperoxideDismutaseIsoforms
...asesinSOD1+/–andjanuskinase/signaltransducerandactivatoroftranscriptase(JAK/STAT)pathwayinSOD2+/–SMCs.PharmacologicalinhibitorsofERK1/2p38andJAK2confirmtheSODgenotype-dependentSMCproliferation.Conclusions—OurresultssuggestthatSOD1andSOD2regulateSMCquiescencebysuppressingdivergentmitogenicsigna...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第5期IsolationandCharacterizationofVasohibin-2asaHomologueofVEGF-InducibleEndothelium-DerivedAngiogenesisInhibitorVasohibin
...reobtainedfromKURABOandwereroutinelyculturedontypeIcollagen-coateddishes(IWAKI)inendothelialbasalmediumcontainingendothelialcellgrowthsupplementsand10%fetalbovineserum.ReverseTranscriptase-PolymeraseChainReactionAnalysisandCloningofHumanVasohibin-2cDNATotalRNAwasextractedfromHUVECsbyanacidguanidiniu...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2006年第26卷第5期TranscriptionFactorCHF1/Hey2RegulatesNeointimalFormationInVivoandVascularSmoothMuscleProliferationandMigrationInVitro
...tofRac1ActivationMeasurementofGTP-boundRac1usingacoprecipitationmethodwithPAK-1PBDagarose(UpstateCellSignalingSolutions,LakePlacid,NY)wasperformedaccordingtothemanufacturer’sinstructionswithminormodifications.Briefly,after48to72hoursofserumstarvation,cellswerestimulatedwith5ng/mLofPDGFor50ng/mLofH...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2004年第24卷第11期AtherosclerosisIsAttenuatedbyLimitingSuperoxideGenerationinBothMacrophagesandVesselWallCells
...tory).PlasmaOxidizedLDLlevelsweremeasuredusingtheOxidizedLDLCompetitiveELISAkit(Mercodia).CellCultureandWesternAnalysisIsolationofaorticSMCsandWesternanalysisweredescribedonlineinthesupplementalmaterials.StatisticalAnalysisDataarepresentedasmean±SEM.Alldatawereanalyzedusing1-wayANOVAfollowedb...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2007年第27卷第12期DifferentialEffectsofVasodilatoryProstaglandinsonFocalAdhesions,CytoskeletalArchitecture,andMigrationinHumanAorticSmoothMuscleCells
FromtheMolekularePharmakologie(C.B.,J.W.F.),InstitutfürPharmakologieundKlinischePharmakologie(A.-A.W.,K.S.),KlinikfürGastroenterologie,HepatologieundInfektiologie(U.A.,J.G.B.),HeinrichHeineUniversit?tDüsseldorf;andtheInstitutfürPathophysiologie,Universit?tsklinikumEssen(B.L.),Germany.Corresponde...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第1期Phospholemman-PhosphorylationMediatesthe-AdrenergicEffectsonNa/KPumpFunctioninCardiacMyocytes
...fpump-mediated[Na]idecline.21MyocyteswereNa-loadedbyinhibitingtheNa/KpumpinaKfreesolutioncontaining(mmol/L):145NaCl,2EGTA,10HEPES,and10glucose(pH=7.4).[Na]ideclinewasmeasuredonpumpreactivationinsolutioncontaining(mmol/L):140TEA-Cl,4KCl,2EGTA,1MgCl2,10HEPES,and10glucose(pH=7.4).NCXisblockedinthisNa-f...
醫源資料庫;在線期刊;循環研究雜誌;2005年第95卷第8期Relationbetweenthefattyacidcompositionofperipheralbloodmononuclearcellsandmeasuresofimmunecellfunctioninhealthy,free-livingsubjectsaged
SamanthaKew,TapatiBanerjee,AnneMMinihane,YvonneEFinnegan,ChristineMWilliamsandPhilipCCalder1FromtheInstituteofHumanNutrition,UniversityofSouthampton,Southampton,UnitedKingdom(SK,TP,andPCC),andtheHughSinclairUnitofHumanNutrition,SchoolofFoodBiosciences,UniversityofReading,Reading,UnitedKingdom(AMM,YE...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2003年77卷第5期