AdenoviralexpressionofNHERF-1inNHERF-1nullmouserenalproximaltubulecellsrestoresNpt2aregulationbylowphosphatemediaandparathyroidhormone
...virus-GFPoradenovirus-GFP-NHERF-1wasextractedusingRNAqueous-MidiKit(Ambion,Austin,TX).First-strandcDNAsynthesisfromtotalRNAwasdoneusingrandomhexamerswiththeRETROscriptreversetranscriptionkitwithSuperTaqpolymerase(Ambion).RelativequantitationofNpt2amRNAwasdoneusingtheiCycleriQtm(Bio-Rad)with15Sasanin...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第10期Physicalactivityasametabolicstressor
...ologyandHealthEducationandDivisionofNutritionalSciences,UniversityofTexasatAustin.2PresentedattheworkshopRoleofDietarySupplementsforPhysicallyActivePeople,heldinBethesda,MD,June34,1996.3AddressreprintrequeststoEFCoyle,UniversityofTexasatAustin,Room222,BellmontHall,Austin,TX78712.ABSTRACTBothph...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年72卷第2期ExpressionanddistributionofHuRduringATPdepletionandrecoveryinproximaltubulecells
...asmicandnuclearextractsfromLLC-PK1cellswereobtainedusingthePariskit(Ambion,Austin,TX).Equivalentsamplesfromeachfractionwereloadedinprecastpolyacrylamidegels(Bio-Rad)andvisualizedbyWesternblottinganalysis.Glyceraldehyde-3-phosphatedehydrogenase(GAPDH)wasdetectedusingamonoclonalantibodyfromAmbion,whil...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第12期TRPV1b,aFunctionalHumanVanilloidReceptorSpliceVariant
...RNA,preparedfromthehTRPV1bcDNAusingT7RNApolymerase(mMessagemMachine;Ambion,Austin,TX),andtwo-electrodevoltage-clamprecordingswereperformed7to10dayslater(Welchetal.,2001).OocyteswereconstantlysuperfusedwithaCa2+-freebuffercontaining90mMNaCl,1mMKCl,2.4mMNaHCO3,1mMMgCl2,10mMHEPES,and0.1mMBaCl2thatwasad...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第4期AcutedecreaseinrenalmicrovascularPO2duringacutenormovolemichemodilution
...±SD,unlessotherwiseindicated.Labview6.1software(NationalInstruments,Austin,TX)wasusedtodevelopasoftwareenvironmenttoallowdataacquisitionandanalysisofthephosphorescencedecaycurves.StatisticswereperformedusingGraphPadPrismversion4.0forWindows(GraphPadSoftware,SanDiego,CA).Fordataanalysiswithine...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第2期Neuronalcalciumsensor-1enhancementofInsP3receptoractivityisinhibitedbytherapeuticlevelsoflithium
...edizinBerlin,Berlin,Germany.5CenterforLearningandMemory,UniversityofTexasatAustin,Austin,Texas,USA.6CenterforCellularandMolecularBiology,Hyderabad,India.7ObstetricsandGynecology,YaleUniversity,NewHaven,Connecticut,USA.AbstractRegulationanddysregulationofintracellularcalcium(Ca2+)signalingviatheinosi...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第6期AVP-inducedVIT32geneexpressionincollectingductcellsoccursviatrans-activationofaCREinthe5‘-flankingregionoftheVIT32gene
...omH441cellmRNAusinganRNAligase-dependentRACEkit(FirstChoiceRLM-RACE,Ambion,Austin,TX),aspreviouslydescribed(17).ThesynthesizedcDNAwassubjectedtonestedPCRinsequentialreactionsusingtwoadaptor-specificprimersandtwogene-specificprimerscomplementarytoaregioninthe5‘-endoftheknowntranscript(hVIT32_5‘RA...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第9期CharacterizationofaSerodiagnosticComplementFixationAntigenofCoccidioidesposadasiiExpressedintheNonpathogenicFungusUncinocarpusreesii
...son,Madison,WI53705.Presentaddress:CollegeofPharmacy,TheUniversityofTexasatAustin,Austin,TX78758.REFERENCESBowman,B.H.,T.J.White,andJ.W.Taylor.1996.Humanpathogenicfungiandtheirclosenonpathogenicrelatives.Mol.Phylogenet.Evol.6:89-96.Coligan,J.,B.Dunn,H.Ploegh,D.Speicher,andP.Wingfield.1995.Currentpro...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第11期The3‘-untranslatedregionoftheSte20-likekinaseSLKregulatesSLKexpression
...logyreagentswerefromInvitrogen,NewEnglandBiolabs(Mississauga,ON),orAmbion,(Austin,TX).Mouseanti-GFPantibodywaspurchasedfromSantaCruzBiotechnology(SantaCruz,CA).Mouseanti-FLAGantibodywasfromSigma-AldrichCanada(Mississauga,ON).ThecDNAoffull-lengthhumanSLKplusthe3‘-UTRwaskindlyprovidedbyDr.T.Nagase(K...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第2期Regulationofsodium-dicarboxylatecotransporter-3fromwinterflounderkidneybyproteinkinaseC
...anscriptionwascarriedoutusingtheT7RNApolymerase(mMessagemMachinekit,Ambion,Austin,TX).ThecRNAwaspurifiedbyphenol-chloroformextraction,resuspendedinwater,andadjustedtoafinalconcentrationof1µg/µl.Site-directedmutagenesis.Apredictionoftheputativephosphorylationsiteswasmadebyusinghttp://www....
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第1期