ProteinKinaseCeCDependentPhosphorylationofSyndecan-4RegulatesCellMigration
...uffercontaining5ehistoneH1(anexogenousPKCsubstrate;Calbiochem)and30e藽iof[32P]ATP(Perkin-Elmer).After30minutes,thereactionwasterminatedandthesampleresolvedby4%to12%gradientSDS-PAGE.Phosphorylatedhistonewasdetectedbyautoradiographyandquantitatedbydensitometry.PKCkinaseactivitywasperformedessentially...
醫源資料庫;在線期刊;循環研究雜誌;2005年第95卷第9期Generationoftransgenicmicewithmildandsevereretinalneovascularisation
...screenedbySouthernblotanalysisofEcoRIdigestedtailDNAandprobedwitha1.1kb[–32P]dCTPlabelledEcoRIfragmentofthemouserhodopsinpromoterfrompcDNA.opsin.VEGF(A).Lane1,lambdaDNAcontrol;lanes2and3,noDNAcontrol.(C)AgarosegelelectrophoresisofPCRproductsfromthedifferentlines.Lane1,noprimercontrol;lane2,noDNAco...
醫源資料庫;在線期刊;英國眼科學雜誌;2005年第89卷第7期SolubleAdenylylCyclaseRevealstheSignificanceofcAMPCompartmentationonPulmonaryMicrovascularEndothelialCellBarrier
...ol/Lcalcium(determinedusingFabiatofreeioncalculator).31Reactionscontained[-32P]ATP(1x106cpm)withorwithout100eol/Lforskolinandinitiatedbyadditionof0.15mgofprotein,proceededfor30minutesat30°Candterminatedusing200e蘈30%trichloroaceticacid.Recoverywasmonitoredusinginternalstandard[2,8-3H]cAMP(15000cpm...
醫源資料庫;在線期刊;循環研究雜誌;2006年第96卷第3期百日咳毒素S1亞單位的分子修飾及在重組桿狀病毒中的表達
...原位及斑點雜交 採用隨機引物標記技術將S1/2DNA片段經32P標記後用作探針,檢測重組菌落的rBVs中的S1DNA。菌落的原位雜交按常規方法進行。斑點雜交時先將rBV感染的細胞用1mol/LNaOH裂解後加到硝酸纖維素膜上,進行雜交。 1.7...
合作平臺;醫學論文;臨牀醫學與專科論文;檢驗醫學第十三節 腫瘤的病因學和發病學
...藥物可在應用相當長時間以後誘發第二種腫瘤,如在化學治療痊癒或已控制的白血病、何傑金淋巴瘤和卵巢癌的病人,數年後可能發生第二種腫瘤,通常是粒細胞性白血病。某些使用烷化劑的非腫瘤病人,如類風溼性關節炎和Weg...
醫源資料庫;醫源圖書館;教材類;病理學PartialOff-LoadingofLongitudinalTensionInducesArterialTortuosity
...poligonucleotide(tocontrolforvariabilityinloadingofwells)wereincubatedwith[32P]dCTPand10UofKlenowpolymerase(PharmaciaBiotech).DNAwaselectrophoresedona1.8%agarosegelandblottedontoHybondnylonmembrane(ICN).End-labeledDNAfragmentswerevisualizedbyexposingthemembranestoKodakX-OMARfilm.StatisticalAnalysisS...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第5期ConstitutiveActivationofrac1ResultsinMitochondrialOxidativeStressandInducesPrematureEndothelialCellSenescence
...llularRNAwasextractedbyastandardprotocol,electrophoresed,andhybridizedwitha32P-labeledsingle-strandDNAprobeofhumanplasminogenactivatorinhibitor-1(PAI-1)11or18SrRNA.Quantitativevaluesareexpressedafternormalizationwith18S.MitochondrialOxidativeStressMitochondrialROSweremeasuredwiththemitochondrial-spe...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2003年第23卷第1期cAMP-dependentstabilizationofphosphoenolpyruvatecarboxykinasemRNAinLLC-PK1-F+kidneycells
...yelementsandmayinvolvephosphorylationofAUF1.MATERIALSANDMETHODSMaterials.[-32P]dCTP(3,000Ci/mmol)wasobtainedfromMPBiologicals.TheDualLuciferaseReporterAssayKitwasobtainedfromPromega.Forskolinanddoxycycline(Dox)wereobtainedfromSigma.HygromycinBwaspurchasedfromMediatech.TheGENECLEANKitwaspurchasedfrom...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第3期TheMajorHistocompatibilityComplexGeneRegionandSarcoidosisSusceptibilityinAfricanAmericans
...flabeling.Inthefirstcase,oneprimerineachreactionwaslabeledatthe5‘endwith[32P]ATP(20).Theamplificationreactionincluded10ngDNA;1to2pmoleachprimer;50mMTris–hydrogenchloride(pH8.3),2.5mMmagnesiumchloride,0.05%Tween-20,0.05%NP-40,20mMeachdeoxynucleotidetriphosphate,and1UTaqDNApolymerase(Promega,Madis...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2003年第167卷第3期Expressionprofileofahumaninduciblenitricoxidesynthasepromoterreporterintransgenicmiceduringendotoxemia
...am,Piscataway,NJ),andultravioletcross-linked.TheDNAblotswerehybridizedwith[32P]dCTP-labeled466bpSmaI/SacIfragmentanda309bpStuI/StuIfragmentfrom8,296/+86hiNOS/EGFPtodetectthehiNOS5‘-flankingregion.TheintensityofthesetwobandsfromthesamemouseonthesameblotwasdeterminedusingtheKODAKImageStation1000.The...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第1期