ShortPolybasicPeptideSequencesArePotentInhibitorsofPC5/6andPC7:UseofPositionalScanning-SyntheticPeptideCombinatorialLibrariesasaToolfort
...CLs)arepowerfulmoleculartoolstoidentifyenzymesubstrateandpotentinhibitorysequencesandalsotoprovidecrucialinformationaboutactivesitedeterminants.PS-SPCLshavebeensurveyedforfurin,proproteinconvertase(PC)2,PC1/3,andPACE4andprovenefficienttoidentifypotentpeptidylinhibitorsinthelownanomolarrangeforfurina...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第1期-AdrenergicReceptorStimulationPromotesGsInternalizationthroughLipidRafts:AStudyinLivingCells
...usionproteinwasdevelopedbyinsertinggreenfluorescentproteinintotheinternalsequenceofGs.ThisGs-GFPfusionproteinbindsGTPinresponsetoagonist,activatesadenylylcyclase,isappropriatelyexpressedattheplasmamembrane,andexhibitstraffickingandsignalingbehavioridenticalwiththatofthewild-typeGs(YuandRasenick,2002...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第5期ImmaturemonocytesacquireantigensfromothercellsinthebonemarrowandpresentthemtoTcellsaftermaturingintheperiphery
...renotrecognizedtodirectlyparticipateinantigenpresentation.Wedevelopedtechniquestolabelmousemonocytesubsetswithparticulatetracersinvivo.Gr-1lobutnotGr-1himonocyteswerestablylabeledbyintravenousinjectionof0.5-μmmicrospheres.Gr-1himonocytescouldbelabeledwhenthemicrosphereswereinjectedaftersystemicdepl...
醫源資料庫;在線期刊;實驗醫學雜誌;2006年第203卷第3期Restorationofhypothalamiclipidsensingnormalizesenergyandglucosehomeostasisinoverfedrats
...gbluntsthecentraleffectsoffattyacidsonfoodintakeandglucoseproduction.Thisacquireddefectinnutrientsensingcouldcontributetotherapidonsetofhyperphagiaandinsulinresistanceinthismodel.Hereweexaminedwhethercentralinhibitionoflipidoxidationissufficienttorestorethehypothalamiclevelsoflong-chainfattyacyl-CoA...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第4期TranscriptionalRegulationofBrain-DerivedNeurotrophicFactorintheAmygdaladuringConsolidationofFearMemory
...eviouslythatbrain-derivedneurotrophicfactor(BDNF)signalingintheamygdalaisrequiredfortheconsolidationoffearmemory.ThisstudyisdesignedtocharacterizethesignalcascadesbywhichfearconditioningmodulatestranscriptionalandtranslationalexpressionofBDNF.Real-timereversetranscription-coupledpolymerasechainreact...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第8期Morethancolocalizingwithpolycystin-1,polycystin-Lisinthecentrosome
...alsobeenfoundintheprimarycilia(38,41,56)and,inrenalcells,thislocalizationrequiresPC1(38).Accumulatingevidencesuggeststhatpolycystins-1and-2interacttoformachannelcomplexthatisinvolvedbothintheinitiationandmaintenanceofaterminallydifferentiatedstateoftubularepithelialcells,andmechanosensationinrelatio...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第8期AninversioninvolvingthemouseShhlocusresultsinbrachydactylythroughdysregulationofShhexpression
...sing11.7Mb.Thedistalbreakpointis13.298kbupstreamofShh,separatingthecodingsequencefromseveralputativeregulatoryelementsidentifiedbyinterspeciescomparison.TheinversionresultsinalmostcompletedownregulationofShhexpressionduringE9.5–E12.5,explainingthehomozygousphenotype.AtE13.5andE14.5,however,Shhisup...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第4期IdentificationofMedicallyImportantMoldsbyanOligonucleotideArray
...thantifungalagents.Onthebasisoftheinternaltranscribedspacer1(ITS1)andITS2sequencesoftherRNAgenes,anoligonucleotidearraywasdevelopedtoidentify64species(32genera)ofclinicallyimportantfilamentous(ordimorphic)fungi.These64speciesincludedfungicausingsuperficial,cutaneous,subcutaneous,andinvasiveinfection...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期ANovelMultivalentLigandThatBridgestheAllostericandOrthostericBindingSitesoftheM2MuscarinicReceptor
...atundesirablemuscarinicreceptorsubtypesoutsidetheintendedorgansystem.Highsequencehomologywithintheorthostericsite,acrossthefivereceptorsubtypes,makesthesynthesisoftissue-specificorsubtype-specificdrugschallenging(Hulmeetal.,1990).Adrugwithspecificityforagivensubtypeortissueispredictedtohaveincreased...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第8期CD+RenalProgenitorCellsContributetoTumorAngiogenesis
...agradedseriesofmeshestoseparatethecellcomponentsfromstromaandaggregates.Aliquotsofthecellsuspensionweresubjectedtofluorescence-activatedcellsortinganalysisforquantificationofCD133+cells.CD133+cellswereisolatedbymagneticcellsorting,usingtheMACSsystem(MiltenyiBiotec,Auburn,CA).18CD133+cellswereplatedo...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第12期