AlterationsofGeneExpressionintheDevelopmentofEarlyHyperplasticPrecursorsofBreastCancer
...themicroarrayexperimentswaspurifiedfromthesamplesusingtheOptimumkit(Ambion,Austin,TX),whichisbasedonproteinaseKdigestionfollowedbyphaseseparationinaproprietarymicrofiltercartridgeandincubationwithDNasetoremovecontaminatinggenomicDNA,yieldingfrom50to300ngpersample.ThepurifiedtotalRNAwasthenlinearlyam...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第7期MissensemutationsinNa+:HCO3
...Palone(noNBCinsert)cRNAsweregeneratedusingmMESSAGEmMACHINET7Kit(fromAmbion,Austin,TX)accordingtothemanufacturer‘sinstruction.FiftynanolitersofcRNA(0.5μg/μl)wereinjectedwithaDrummond510microdispenserviaasterileglasspipettewithatipof2030μm.Afterinjection,theoocytesweremaintainedinasolutionofthefo...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第7期Functionalrolesofcationicaminoacidresiduesinthesodium-dicarboxylatecotransporter3(NaDC-3)fromwinterflounder
...iction.InvitrotranscriptionwascarriedoutusingaT7mMessagemMachinekit(Ambion,Austin,TX).AfterpurificationthecRNAbyphenol-chloroformextraction,thecRNAwasresuspendedinwaterandadjustedtoafinalconcentrationof1µg/µl.Site-directedmutagenesis.Thebasicaminoacids(arginine,histidine,andlysine)locate...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第12期TheCablesGeneonChromosomeqIsSilencedbyPromoterHypermethylationandAllelicLossinHumanColorectalCancer
...er??sinstructions.AllRNAsampleswereDNase-treatedusingtheDNA-freekit(Ambion,Austin,TX)andreversetranscribedtocDNAusingtheSuperScriptIIIFirstStrandSynthesiscDNAkit(Invitrogen).Real-timePCRwasperformedbyCepheidSmartCyclerIIusingLightcyclerTaqmanMasterkit(Roche,Mannheim,Germany).TaqManprobesweresynthesi...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第11期PioglitazoneandRosiglitazoneDecreaseProstaglandinE2inNon–Small-CellLungCancerCellsbyUp-Regulating15-HydroxyprostaglandinDehydrogenase
...-base-pairscrambledsequence)SilencersmallinterferingRNA(siRNA)(Ambion,Inc.,Austin,TX)usingTransMessengertransfectionreagent(QIAGEN,Valencia,CA)atdifferentRNA/transfectionreagentratios.Inallconditions,weobservedasignificantsuppressionof15-PGDHexpression(50–90%inhibition)byusing15-PGDHpolyclonal...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第6期Thecharacterizationof5-integrinexpressionontubularepitheliumduringrenalinjury
...AAUCCAGtt3‘,antisense5‘CUGGAUUCCCUAAUUAUGGtc3‘)orGAPDHcontrol(Ambion,Austin,TX)usingLipofectamine2000asperthemanufacturer‘sprotocol(Invitrogen,Carlsbad,CA).Cellswereharvestedattheindicatedtimepoints,andproteinlysateswereanalyzedbyimmunoblottingasdescribedabove.Animalmodelofunilateralureteric...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第2期ANovelRoleofMyosinVIinHumanProstateCancer
...romtissuesorculturedcellswereamplifiedonceusingtheMessageAmpaRNAkit(Ambion,Austin,TX)usinganinputof500ngoftotalRNA,andlabeledbydirectincorporationofCy3-dUTP(AmershamPharmacia,Piscataway,NJ)inareversetranscriptionreactionusingrandomprimersandSuperscriptIIreversetranscriptase(Invitrogen,Carlsbad,Calif...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第11期TranslationalRegulationofRatMultidrugResistance-AssociatedProtein2ExpressionIsMediatedbyUpstreamOpenReadingFramesinthe5‘UntranslatedRegion
...eledMrp2probewaspreparedaccordingtotheinstructionsofMAXIscriptT7kit(Ambion,Austin,TX)usingthe280-basepairfragmentastemplate.RPAwasperformedaccordingtomanufacturer‘sinstructions(RPAIIIKit;Ambion).Inbrief,totalRNAwasincubatedwiththeMrp2probeinthemixturecontaining0.5Mammoniumacetateand2.5volumesofeth...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第1期MolecularEpidemiologyofShigellaflexneriinaLong-StayPsychiatricNursingCenterduring2001to2003
...thencomparedforsimilaritybyusingBionumericssoftware(version3.0;AppliedMath,Austin,Tex.).OnthebasisofthePearsoncorrelationcoefficient,adendrogramwasconstructedbytheunweightedpairgroupmethodwitharithmeticaveragesclustering.Atoleranceforthebandingmigrationdistanceof1.0%wasappliedduringthecomparisonofth...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第3期AdenosineA2AReceptorStimulationIncreasesAngiogenesisbyDown-RegulatingProductionoftheAntiangiogenicMatrixProteinThrombospondin1
...arlsbad,CA).ResidualgenomicDNAwasremovedbyincubatingtheRNAwithDNase(Ambion,Austin,TX).ThequantityoftotalRNAfromeachconditionwasmeasuredbyHitachiU2010spectrophotometer(Hitachi,Brisbane,CA).ThequalityoftheRNAwasverifiedbygelelectrophoresis.ThetotalRNAyieldfrom1x106culturedcellswas8to15e.ReverseTranscr...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第5期