UseofShell-VialCellCultureAssayforIsolationofBacteriafromClinicalSpecimens:13YearsofExperience
...edearlyantigendetection(22).Thisdiagnostictoolisbecomingoneofthemostcommonlyusedtechniquesinclinicalvirologylaboratoriessinceitreducesturnaroundtime(19).Foralongtime,Rickettsiaspp.,whicharestrictintracellulargram-negativebacteria,wereisolatedusingembryonatedeggand/oranimalinoculations(44).AstheNatio...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第10期SimultaneousDetectionofImmunoglobulinA(IgA)andIgMAntibodiesagainstHepatitisEVirus(HEV)IsHighlySpecificforDiagnosisofAcuteHEVInfection
...nti-HEVIgAandanti-HEVIgMassaysandevaluatedtheirabilitytodiagnosehepatitisEbyusingserumsamplesfrom68patientswhoweresubsequentlymolecularlydiagnosedashavinghepatitisEandfrom2,781individualswhowereassumedtonothavebeenrecentlyinfectedwithHEVasnegativecontrolsinanattempttoimprovetheserologicaldiagnosisof...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第1期DensityoftheNotchligandDelta1determinesgenerationofBandTcellprecursorsfromhematopoieticstemcells
...atopoieticprecursorsatmultiplematurationalstagesandsubstantiatethepreviouslyunrecognizedabilityofDelta1toenhancethedevelopmentofbothearlyBandTprecursorcells.ThedetectionofNotchreceptorsinhematopoieticprecursorssuggestedaroleforNotchsignalinginearlyhematopoieticdevelopment,whereasthepotentialroleofNo...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第9期Pharmacologicalmanipulationofcelldeath:clinicalapplicationsinsight?
...tsoncancercells,formingthebasisofmanyestablishedapproachestocancertherapy.Byunderstandingandtargetingsuchpathwaysspecifically,wecanimprovetherapieswithfewersideeffects.TherapeuticcelldeathinductionatthepreclinicalandclinicallevelsMostcytotoxicagentsusedinanticancerchemotherapyinduceapoptosis,althoug...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第10期EtiologyofDiarrheainYoungChildreninDenmark:aCase-ControlStudy
...rotoxigenic,enteroinvasive,andenteroaggregativeEscherichiacoliweredetectedbyusingcolonyhybridizationwithvirulencegeneprobesandserotyping.Parasitesweredetectedbymicroscopy.Overall,apotentialpathogenwasfoundin54%ofcases.Morecasesthancontrolswereinfectedwithrotavirus,Salmonella,norovirus,adenovirus,Cam...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期LatexAgglutinationTestforMonitoringAntibodiestoAvianInfluenzaVirusSubtypeH5N1
...pretreatmentofserumspecimenstoeliminatenonspecificinhibitors.Anothercommonlyusedmethodistheenzyme-linkedimmunosorbentassay(3),whichtakesseveralhoursatleastandneedstrainedpersonnelandspecialequipment.Herewereportthedevelopmentandevaluationofalatexagglutinationtest(LAT)basedonpolystyrenebeadssensitize...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第4期E47isrequiredforV(D)Jrecombinaseactivityincommonlymphoidprogenitors
...yattheCLPstageofdevelopment(2,11).E2AisexpressedatlowlevelsinLSKsandishighlyup-regulatedduringprogressiontotheBlineage(1,12),butitremainsunknownwhetherE2AactivityisrequiredforrecombinationinCLPs.E2AinitiatesakeytranscriptionalcascadeinvolvingearlyBcellfactor(EBF)andpax5thatleadstotheexpressionofline...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第202卷第12期Mappingquantitativetraitlociinhumans:achievementsandlimitations
...aledunforeseencomplexitiesandhaveresultedinmanyfailures(5-6),andtherearemanyunresolvedissuespertainingtostudydesignandstatisticalanalyses(7-8).Inhumans,andinotheroutbredspecies,QTLmappinghasonlyhadlimitedsuccess(seeTastesensitivitytophenylthiocarbamideforasuccessstory).Inthisreview,weshallfocusonlyo...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第6期TransferandEvaluationofanAutomated,Low-CostReal-TimeReverseTranscription-PCRTestforDiagnosisandMonitoringofHumanImmunodeficiencyVirusType1
...rtechniqueandtheVersantassay.TheTaqManreal-timePCR($12pertest)isnowroutinelyusedtomonitorHIV-1infectioninourlaboratory.Thistechnologyshouldbefurtherevaluatedinlimited-resourcecountrieswherestrainsotherthanCRF02areprevalent.INTRODUCTIONInsub-SaharanAfrica,theincreasedaccesstoantiretroviral(ARV)drugs(...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第6期Splenectomyinactivatesthecholinergicantiinflammatorypathwayduringlethalendotoxemiaandpolymicrobialsepsis
...ressionofTNFmRNAwasdeterminedbyquantitativereal-timePCRusingTaqMantechnologyusingtheEurogentec‘sRTqPCRmastermixandtheABIPRISM7700SequenceDetectionSystem.Optimalconcentrationsofprimers,probes,andtheRNAwerestandardized.TNFprimer(forward:GCTGCCCCGACTATGTGCT,reverse:GACTTTCTCCTGGTATGAAATGGC)wasaddedat...
醫源資料庫;在線期刊;實驗醫學雜誌;2006年第203卷第7期