Sodium-dependentmethotrexatecarrier-1isexpressedinratkidney:cloningandfunctionalcharacterization
...theadaptorswereperformedusingaMarathoncDNAamplificationkit(Clontech,Heidelberg,Germany)accordingtothemanufacturer‘sinstructionsandaspreviouslydescribed(23).Inbrief,thefirst-strandcDNAsynthesiswasstartedwith1µgpolyA+-RNAand10pmolofthecDNAsynthesisprimerfor60minat42°C.Second-strandcDNAsynt...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第3期Temporalexpressionprofileanddistributionpatternindicatearoleofconnectivetissuegrowthfactor(CTGF/CCN-2)indiabeticnephropathyinmice
...eterminedleptindeficiencyinhomozygousob/obmicecausesinsulinresistanceandhyperglycemiaalreadyatayoungage(58).Transgenicmiceexpressinghumanamyloidpolypeptideintheirpancreaticisletsdevelopisletamyloid,particularlymaleanimalswhencrossedbackwithob/obmice(22).FemalehIAPP-transgenicob/obmice(n=5,C57BL/6Jba...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第6期Infantvisionandretinalfunctioninstudiesofdietarylong-chainpolyunsaturatedfattyacids:methods,results,andimplications
...nshadabnormallylowDHAlevelsinphotoreceptormembranesandlowelectroretinogram(ERG)amplitudes,andthattheamplitudeofthislight-evokedretinalresponseincreasedinproportiontotheamountofn3fattyacidsaddedtothediet(2).Changesinretinalfunctionhavebeenconfirmedbysubsequentstudiesinrats(3,4),guineapigs(5),no...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年71卷第1期AggregatedMyocilinInducesRussellBodiesandCausesApoptosis
...wever,manyglaucoma-causingmutantMYOCshavebeenfoundtobemisfoldedandtoformdetergent-resistant,secretion-incompetentaggregates.19,23-27ThesecretionblockofPOAG-causingmutantMYOCscouldbeovercomebyloweringthecellculturetemperaturefrom37to30??C,aconditionknowntoimproveproteinfolding.17,19Increasingevidence...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第1期DiagnosticValueofMeasuringEpstein-BarrVirus(EBV)DNALoadandCarcinoma-SpecificViralmRNAinRelationtoAnti-EBVImmunoglobulinA(IgA)andIgGAntib
...ty,whereasEBNA1mRNAmaybefoundinbothcirculatinglatentlyEBV-infectedBcellsundergoingcelldivision(23)andintactNPCtumorcells.EBVmRNAdetectioninperipheralbloodhashithertonotbeenstudiedasanNPCmarker,butwerecentlyshoweditsdiagnosticpotentialinnasopharyngealtissuebiopsyspecimens(5,21)andnasopharyngealbrushi...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第7期AcquisitionoffulleffectorfunctioninvitroparadoxicallyimpairstheinvivoantitumorefficacyofadoptivelytransferredCD8+Tcells
...ermediateeffector,andeffectorTcells.Meanfluorescenceintensity(MFI)valuesaftergatingonCD8+cellsareshown.Datashownarerepresentativeof3independentexperiments.(B)Functionaldifferencesinnaiveandeffectorsubgroups.IFN-andIL-2releaseandcytotoxicassayagainsthgp10025–33–pulsedMCA-205targets.MCA-205cellspl...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第6期TonsillarhomingofEpstein-Barrvirus–specificCD8+Tcellsandthevirus-hostbalance
...annheim,Mannheim,Germany.AbstractPatientswithinfectiousmononucleosis(IM)undergoingprimaryEBVinfectionshowlargeexpansionsofEBV-specificCD8+Tcellsintheblood.WhilelatentinfectionoftheBcellpoolisquicklycontrolled,virussheddingfromlyticallyinfectedcellsintheoropharynxremainshighforseveralmonths.Wetherefo...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第9期Cholesterolbinding,efflux,andaPDZ-interactingdomainofscavengerreceptor–BImediateHDL-initiatedsignaling
...soinducesmultiplecellularsignals,whichinendotheliumoccurthroughSR-BIandconvergetoactivateeNOS.TodeterminethemolecularbasisofasignalingeventinducedbyHDL,weexaminedtheproximalmechanismsinHDLactivationofeNOS.Inendothelialcells,HDLandmethyl-?-cyclodextrincausedcomparableeNOSactivation,whereascholesterol...
醫源資料庫;在線期刊;臨牀研究雜誌;2005年第115卷第4期MyristylTrimethylAmmoniumBromideandOctadecylTrimethylAmmoniumBromideAreSurface-ActiveSmallMoleculeDynaminInhibitorsthatBlockEndocytosisMedia
...n-specific,anditisexpressedatmuchhigherlevelsthaneitheroftheotherdynamins(Fergusonetal.,2007).Dynaminisa96-kDaGTPaseenzymeinvolvedinmembraneconstrictionandfissionduringRMEandSVE.Atalatestageoftheprocess,dynaminassemblesintoringstoformacollarorhelixaroundtheneckoftheinvaginatingvesicles.UponGTPhydrol...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第12期