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Negativeregulationbythyroidhormonereceptorrequiresanintactcoactivator-bindingsurface
...elsandpituitaryTshbmRNAlevelswereinappropriatelyelevatedcomparedwiththoseofWTanimals,andL-T3treatmentfailedtosuppressserumTSHandpituitaryTshbmRNAlevels.Therefore,theAF-2domainofTR-?isrequiredforpositiveand,paradoxically,fornegativeregulationbyTHinvivo.IntroductionThesynthesisofthyrotropin-releasingh...
醫源資料庫；在線期刊；臨牀研究雜誌；2005年第115卷第9期 -
Cardiac-specificoverexpressionofAT1receptormutantlackingGq/Gicouplingcauseshypertrophyandbradycardiaintransgenicmice
...dependentandunconventionalGprotein–independentmechanisms.WeinvestigatedhowthesedifferentmechanismsactivatedbyAT1receptorsaffectgrowthanddeathofcardiacmyocytesinvivo.Transgenicmicewithcardiac-specificoverexpressionofWTAT1receptor(AT1-WT;Tg-WTmice)oranAT1receptorsecondintracellularloopmutant(AT1-i2m...
醫源資料庫；在線期刊；臨牀研究雜誌；2005年第115卷第11期 -
CharacterizationofaNovelPKAPhosphorylationSite,Serine-2030,RevealsNoPKAHyperphosphorylationoftheCardiacRyanodineReceptorinCanineHeartFailu
...tesotherthanserine-2808,wegeneratedasinglepointmutation,S2808A,inRyR2.RyR2(wt)andtheS2808AmutantwereexpressedinHEK293cellsandpulleddownbyGST-FKBP12.6.Thepulled-downRyR2(wt)andS2808AproteinswerephosphorylatedbyPKAusing[-32P]ATP.Figure1AbshowsthattheRyR2(wt)proteinwasphosphorylatedbyPKA(lane1),butnotb...
醫源資料庫；在線期刊；循環研究雜誌；2005年第95卷第4期 -
HepaticATP-BindingCassetteTransporterA1IsaKeyMoleculeinHigh-DensityLipoproteinCholesterylEsterMetabolisminMice
...estigation.OverexpressionofadenovirallydeliveredABCA1intheliverofwild-type(WT)miceyieldsasignificantincreaseinplasmaHDLcholesterol(HDL-C).4Incontrast,micewithABCA1selectivelydeletedinthelivershowan80%decreaseinplasmaHDL-C.5TheseinvestigationssuggestthattheliveristhemajorsiteofABCA1-mediatedlipidatio...
醫源資料庫；在線期刊；動脈硬化血栓血管生物學雜誌；2006年第26卷第8期 -
噴砂對鈦鑄件表面結構的影響
...研究通過對純鈦鑄件表面經不同條件噴砂後，鑄件減重（Wt）與表面粗糙度（Ra值）的測定及金相觀察，考察噴砂對其表面結構的影響，爲純鈦鑄件的後期表面處理提供實驗依據。 1.材料方法:用鋯英石包埋料作內包埋，磷酸...
合作平臺；醫學論文；臨牀醫學與專科論文；口腔醫學 -
PlasminModulatesVascularEndothelialGrowthFactor-A-MediatedAngiogenesisduringWoundRepair
【摘要】Plasmin-catalyzedcleavageofthevascularendothelialgrowthfactor(VEGF)-AisoformVEGF165resultsinlossofitscarboxyl-terminalheparin-bindingdomainandsignificantlossinitsbioactivity.Littleisknownabouttheinvivosignificanceofthisprocess.Toinvestigatethebiologicalrelevanceoftheproteasesensitivityof...
醫源資料庫；在線期刊；美國病理學雜誌；2006年第168卷第2期 -
Fertilityinluteinizinghormonereceptor-knockoutmiceafterwild-typeovarytransplantationdemonstratesredundancyofextragonadalluteinizinghormoneacti
...e),inwhichtheovariesofprepubertalmicewereorthotopicallyreplacedwithpiecesofWTovaryusingsimilarlytransplantedWTmiceascontrols.Mostovariantransplantsattainednormalendocrinefunctioninbothgroupsofmice,asdemonstratedbynormalageatvaginalopening,estrouscycles,andsexualbehavior.BoththeLuRKOandWTmicerepeated...
醫源資料庫；在線期刊；臨牀研究雜誌；2005年第115卷第7期 -
MMP-12在小鼠腹主動脈瘤中的作用
...脈瘤之前和誘導成功後10周對其進行比較。測定野生型（WT）和MMP-12敲除（MMP-12–/–）小鼠的主動脈直徑變化。通過酪蛋白酶譜法分析小鼠主動脈MMP-12的生成情況，明膠酶譜法檢測MMP-2和MMP-9的表達。應用免疫組織化學方法檢測主...
行業資訊；臨牀快報；心腦血管相關 -
應激狀態下血紅素氧合酶抑制血栓形成研究
...1+/-)，在雜合小鼠子代中用PCR方法篩選出同代野生型小鼠(Wt)以及基因缺失型小鼠(HO-1-/-),用FeCl3刺激鼠頸動脈並記錄栓塞時間,Westernblot及化學法確定實驗動物血小板HO-1、環狀-磷酸鳥苷(cGMP)表達水平，並用常規方法檢測血小板聚集...
行業資訊；臨牀快報；心腦血管相關 -
CofilinmediatesATPdepletion-inducedendothelialcellactinalterations
...ingthecDNAsforwild-typeXenopuslaevisADF/cofilingreenfluorescentprotein[XAC(wt)-GFP],GFP,andtheconstitutivelyactiveandinactiveisoformsXAC(S3A)-GFPandXAC(S3E)-GFP.TherateandextentofcorticalactindestructionandactinaggregateformationwereincreasedinATP-depletedXAC(wt)-GFP-andXAC(S3A)-GFP-expressingcells,...
醫源資料庫；在線期刊；美國生理學雜誌；2006年第289卷第6期