Decreasein3/6NicotinicReceptorsbutNotNicotine-EvokedDopamineReleaseinMonkeyBrainafterNigrostriatalDamage
...dsMaterials.Theradioligands[3H]dopamine(3,4-[ring-2,5,6-3H];30-60Ci/mmol),[125I]RTI-121(2200Ci/mmol),and125I-epibatidine(2200Ci/mmol)wereobtainedfromPerkinElmerLifeandAnalyticalSciences(Boston,MA).-CtxMIIwassynthesizedasdescribedbyCartieretal.(1996)andlabeledwith125I(Whiteakeretal.,2000).HEPESwaspur...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第3期DistinctReceptorActivity-ModifyingProteinDomainsDifferentiallyModulateInteractionwithCalcitoninReceptors
...edtheN-terminal,transmembrane,orC-terminaldomainandexaminedtheseinassaysof[125I]amylinbindingorpeptide-inducedcAMPsignalinginCOS-7cellstransientlytransfectedwithwild-typeorchimericRAMPsandhumanCTRa.Thespecificityofpeptidesincompetitionfor[125I]amylinbindingwasprincipallydictatedbytheN-terminaldomain...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第6期AntifungalActivitiesofTacrolimusandAzoleAgentsagainsttheElevenCurrentlyAcceptedMalasseziaSpecies
...agentsitraconazoleandketoconazoleagainstMalasseziaspeciesinvitro.Weexamined125strainsofthe11currentlyacceptedMalasseziaspeciesbyusingtheagardilutionmethod.Allstrainsofthe11Malasseziaspecieswereverysusceptibletobothazoleagents,withMICsrangingfrom0.016to0.25μg/ml.Tacrolimushadantifungalactivitiesagai...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第6期TheCCR5Receptor-BasedMechanismofActionof873140,aPotentAllostericNoncompetitiveHIVEntryInhibitor
...nyl]-4‘-methyl-1,4‘-bipiperidin-4-yl}methanoneO-ethyloxime(Sch-C;SCH351125),4,6-dimethyl-5-{[4-methyl-4-((3S)-3-methyl-4-{(1R)-2-(methyloxy)-1-[4-(trifluoromethyl)phenyl]ethyl}-1-piperazinyl)-1-piperidinyl]carbonyl}pyrimidine(Sch-D;SCH417,690),4,4-difluoro-N-((1S)-3-{(3-endo)-3-[3-methyl-5-(1-me...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第4期HeterogeneityofNicotinicCholinergicReceptorsinRatSuperiorCervicalandNodoseGanglia
...atidine([3H]EB;55Ci/mmol)wasobtainedfromPerkinElmerLifeSciences(Boston,MA).125I-Epibatidine(125I-EB;545Ci/mmol)and125I-A-85380(700Ci/mmol)weresynthesizedandpurifiedasdescribedpreviously(Musachioetal.,1997;Hortietal.,1999).Rabbitantiseradirectedatbacteriallyexpressedfusionproteinscontainingpartialseq...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第11期InhibitionofLipopolysaccharide-InducedInflammatoryResponsesbyanApolipoproteinAIMimeticPeptide
...ufferchanges).LPS-ASDwasnextiodinatedusingtheiodogenmethod(Pierce,Inc).Free125Iwasseparatedfrom125IeCLPS-ASDbydialysisandcentrifugation(2000g,1hour,5times)usinganUltrafree-MCfilterunit(Millipore,Inc).Seventypercentoftherecoveredcountsweretrichloroaceticacidprecipitable.Radioligand-BindingStudiesThee...
醫源資料庫;在線期刊;循環研究雜誌;2005年第95卷第8期Effectofn-3fattyacidsonthecompositionandbindingpropertiesoflipoproteinsinhypertriglyceridemicpatients
...nrangedfrom84%to95%.LabelingofLDLLDLfromcontrolsubjectswasradiolabeledbythe125Imonochloridemethodin1molglycineNaOHbuffer/LatpH10.0.Theatom-to-moleratioofiodinetoproteinwaskept1.0(21).Afteriodination,theLDLwasdialyzedagainstEagle‘sminimumessentialmedium(MEM)for4handthenstabilizedbytheadditionof1%hu...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年71卷第1期BasicFibroblastGrowthFactor(bFGFFGF-)PotentiatesLeukocyteRecruitmenttoInflammationbyEnhancingEndothelialAdhesionMoleculeExpression
...ExudationduetoenhancedvascularpermeabilitywasquantitatedusingHSAlabeledwith125I,asdescribed.39Analiquotof125I-HSA(5µCi/kg)wasadministeredintravenously2hoursbeforesacrifice.Thecontentof125Ipersiteandof125Iperµlofbloodplasmawasquantified,andcontentinskinsitesexpressedasµlofplasmaalbu...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第3期PolyunsaturatedFattyAcidsInduce-Synuclein-RelatedPathogenicChangesinNeuronalCells
...etectionofinclusionformationbyICCandusingtwodifferent18:3concentrations(ie,125and250µmol/L).WefirsttreatedS-expressingMEScellswith250µmol/L18:3andaskedhowsoonthecellrespondstoPUFAbySoligomerizationandbyinclusionformation.Cellswereconditionedinthespecificmediumforthenumberofhoursindicated...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第12期NegativeRegulationofSuperoxideDismutase-1PromoterbyThyroidHormone
...lsinconditionsofthyroidhormoneexcess.Plasmids.TheTRmutantsF451X,G345R,andGS125TR1werecreatedwiththeuseofQuikChangesite-directedmutagenesiskits(Stratagene,LaJolla,CA)intothepCMXvectorthatencodes461aminoacidsofhTR1sequence.ThemutatedsequencewasverifiedbyDNAsequencingusingSequenasekits(Stratagene).Thef...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第9期