MolecularAnalysisofMicrobialDiversityinAdvancedCaries
...peratureof4°C.ThetriplicatePCRsampleswerecombinedandpurifiedbyusingtheWizardPCRPrepsDNApurificationsystem(Promega,Sydney,NewSouthWales,Australia)beforebeingclonedintolinearizedplasmidsbyusingthepGEM-TEasyvectorsystem(Promega)accordingtothemanufacturer‘sinstructions.Ligatedplasmidsweretransformedi...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第2期TNF-308ModifiestheEffectofSecond-HandSmokeonRespiratoryIllness–relatedSchoolAbsences
...erExpressapplications-basedprimerdesignsoftware(AppliedBiosystems).TheforwardprimerforhumanTNFwas5‘-GAAATGGAGGCAATAGGTTTTGA-3‘,andthereverseprimerwas5‘-GTAGGACCCTGGAGGCTGAAC-3‘.Theminorgroove-bindingprobefortheGallelewas5‘-CCGTCCCCATGCC-3‘,andtheminorgroove-bindingprobefortheAallelewas5...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2005年第171卷第12期DefiningtheNormalBacterialFloraoftheOralCavity
...analyzed.Sitesincludedtonguedorsum,lateralsidesoftongue,buccalepithelium,hardpalate,softpalate,supragingivalplaqueoftoothsurfaces,subgingivalplaque,maxillaryanteriorvestibule,andtonsils.16SrRNAgenesfromsampleDNAwereamplified,cloned,andtransformedintoEscherichiacoli.Sequencesof16SrRNAgeneswereusedtod...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第11期MultilocusSequenceTypingofSwedishInvasiveGroupBStreptococcusIsolatesIndicatesaNeonatallyAssociatedGeneticLineageandCapsuleSwitching
...sistyping,pulsed-fieldgelelectrophoresis,restrictiondigestionpatterntyping(RDP),andrestrictionfragmentlengthpatternanalysis(25-28,30).DiverselineagesamongserotypeIIIisolateshavebeendistinguished,andlineageshavebeensuggestedtodifferintheirvirulencepotentialsandtissuetropisms.However,infewofthesestudi...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期IdentificationofCandidatePeriodontalPathogensandBeneficialSpeciesbyQuantitative16SClonalAnalysis
...himerasbyusingtheChimeraCheckprogramoftheRibosomalDatabaseProjectII(http://rdp.cme.msu.edu/html/).Twenty-threecloneswereidentifiedaschimericsequencesandexcludedfromfurtheranalysis.Sequenceswerealigned,andasimilaritymatrixwasconstructedfromthealignmentsbythemethodofJukesandCantor.Phylogenetictreeswer...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期Rai1duplicationcausesphysicalandbehavioralphenotypesinamousemodelofdup(17)(p11.2p11.2)
...tudieswereperformedinmalemice,sincegenderspecificitywaspreviouslyobservedforDp(11)17/+mice(22).WewereabletorescuemanyofthebehavioralphenotypesobservedinthemaleanimalswithsegmentalaneuploidiesbynormalizingthegenecopynumberinDp(11)17/Df(11)17mice(Figures2and3).Theverticalactivityandcenter/totaldistanc...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第11期StimulationofNa+transportbyAVPisindependentofPKAphosphorylationoftheNa-K-ATPaseincollectingductprincipalcells
...tsgeneratedbyPCR.Serine943wassubstitutedeitherbyalanine(S943A)usingtheforwardprimerCCAGGAGGAATGCAGTCTTCCAGCAGGandthereverseprimerCCTGCTGGAAGACTGCATTCCTCCTGGorbyasparticacid(S943D)usingtheforwardprimerCCAGGAGGAATGACGTCTTCCAGCAGGandthereverseprimerCCTGCTGGAAGACGTCATTCCTCCTGG.Thefull-lengthwild-typeand...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第11期RapidIdentificationofBacteriafromPositiveBloodCulturesbyTerminalRestrictionFragmentLengthPolymorphismProfileAnalysisofthe16SrRNAGene
...sbyusingtheT-RFLPanalysisprogram(TAPT-RFLP)oftheRibosomalDatabaseProjectII(RDP-II)(19).Asindicated,primerswerelabeledatthe5‘endwiththedyes6-carboxyfluorescein(6FAM),4,7,2‘,4‘,5‘,7‘-hexachloro-6-carboxyfluorescein(HEX),orN,N,N‘,N‘-tetramethyl-6-carboxyrhodamine(TAMRA).Allprimerswerepurc...
醫源資料庫;在線期刊;微生物臨牀雜誌;2003年第41卷第8期Thestorysofar:molecularregulationofthehemeoxygenase-1geneinrenalinjury
...iMC,ChristophF,WooJ,IyerS,BrouardS,HeslanJM,PignonP,SoulillouJP,andBuelowR.RDP1258,anewrationallydesignedimmunosuppressivepeptide,prolongsallograftsurvivalinrats:analysisofitsmechanismofaction.MolMed5:820-832,1999.DaiC,YangJ,andLiuY.Transforminggrowthfactor-1potentiatesrenaltubularepithelialcelldeat...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第3期UseofPhylogeneticandPhenotypicAnalysesToIdentifyNonhemolyticStreptococciIsolatedfromBacteremicPatients
...especies.ThisisanoptionatthedatabaseoftheRibosomalDatabaseProjectII(http://rdp.cme.msu.edu/index.jsp).Ourstudydemonstratesthatunequivocalidentificationofclinicalisolatesofnonhemolyticstreptococcicanbeachievedbyphylogeneticanalysisofconcatenatedsequencesoffourhousekeepinggenes,ddl,gdh,rpoB,andsodA,am...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第12期