Galectin-ExpressionCorrelateswithApoptosisofTumor-AssociatedLymphocytesinHumanMelanomaBiopsies
...lyceraldehyde-3-phosphatedehydrogenasewasalsoincubated(clone6C5;AmbionInc.,Austin,TX).Asstandards,2.5µgofrecombinantgal-1andgal-3(PeproTech,RockyHill,NJ)wasused.Afterseveralwashings,filterswereincubatedwithalkalinephosphatase-conjugatedgoatIganti-mouseIgG(JacksonImmunoResearch,WestGrove,PA)and...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第5期RoleoftheOuter-SheetinDivalentCationModulationof7NicotinicReceptors
...2002a).cRNAwaspreparedusingtheT7RNApolymeraseandmMessagemMachinekit(Ambion,Austin,TX)asdescribedbythemanufacturer.Oocyteswereinjectedwith20ngofcRNAandincubatedat18°CinND96(5mMHEPES,96mMNaCl,2mMKCl,1mMMgCl2,and1.8mMCaCl2,pH7.5)for2to5daysbeforeuse.DataCollectionandAnalysis.Oocytesweresuperfusedin...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第7期PlateletInhibitionbyInsulinIsAbsentinType2DiabetesMellitus
...haemicevents(CAPRIE).Lancet.1996;348:1329-1339.YangJ,WuJ,JiangH,MortensenR,AustinS,ManningDR,WoulfeD,BrassLF.SignalingthroughGifamilymembersinplatelets.Redundancyandspecificityintheregulationofadenylylcyclaseandothereffectors.JBiolChem.2002;277:46035-46042.FerreiraIA,EybrechtsKL,MockingAIM,KronerC,A...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2006年第26卷第2期Fattyaciddesaturaseregulationinadiposetissuebydietarycompositionisindependentofweightlossandiscorrelatedwiththeplasmatriacylglycerolresp
...ion,sampleswerestoredfor24–48hat–20°CinRNALaterICE(Ambion,Austin,TX);88sampleswereexcludedfromRNAisolationbecausetheamountoftissuewasinadequate(5mg);32sampleswereexcludedbecauseofbeingovercontaminatedwithblood,asjudgedbysight;and6sampleswereexcludedbecauseofmislabeling.TotalRNAwasis...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2007年86卷第3期3‘-Untranslatedregionofthetype2bradykininreceptorisapotentregulatorofgeneexpression
...embrane.ChemiluminescentsignalsweredetectedusingBrightStarBioDetect(Ambion,Austin,TX)afterX-rayfilmexposure.Statisticalanalysis.Differencesinluciferaseactivitywerecomparedusing2,Mann-Whitney,orgenerallinearmodelswithrepeated-measurestesting,whereappropriate.Allstatisticaltestsweretwo-sided,andavalue...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第3期PharmacologicalInhibitionandGeneticDeficiencyofPlasminogenActivatorInhibitor-1AttenuatesAngiotensinII/Salt-InducedAorticRemodeling
...iptionPolymeraseChainReactionTotalcardiacRNAwasextractedusingRNAWiz(Ambion,Austin,Tex)andRneasyMidiKit(Qiagen,Valencia,Calif),andtotalaorticRNAwasextractedusingRneasyMiniKit(Qiagen).Reverse-transcriptionwasperformedusingTaqManReverseTranscriptionKit(AppliedBiosystems,Branchburg,NJ).Quantitativereal-...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第2期Parenteralironcompoundssensitizemicetoinjury-initiatedTNF-mRNAproductionandTNF-release
...arosegel(41).RT-PCRwasperformedusinga1stStrandSynthesisKitforRT-PCR(Ambion,Austin,TX),aspreviouslydescribedindetail(51).ThespecificprimersforTNF-andGAPDH(housekeepinggene)weredesignedwith5060%GCcomposition(seeTable1).MultiplexPCRwaspossibleduetotheprimers‘similarannealingtemperaturesbutdissimilars...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第2期Visualmaturationofterminfantsfedlong-chainpolyunsaturatedfattyacidsupplementedorcontrolformulafor12mo
...spholipaseA2ineicosanoidgeneration.AmJRespirCritCareMed2000;161:S325.AustinS,FunkC.Insightintoprostoglandin,leukotriene,andothereicosanoidfunctionsusingmicewithtargetedgenedisruption.ProstaglandinsOtherLipidMediat1999;58:23152.StubbsC,SmithA.Themodificationofmammalianpolyunsaturatedfatty...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2005年81卷第4期CalciumandchloridechannelactivationbyangiotensinII-AT1receptorsinpreglomerularvascularsmoothmusclecells
...erBioproducts,Walkerville,MD)containing10μMFura-2AM(TexasFluorescenceLabs,Austin,TX)andincubatedinthedarkatroomtemperaturefor1h(13,14).Solutionsweretestedforsodiumandpotassiumconcentrationsbyflamespectrophotometry(InstrumentationLaboratory),osmolality(Wescor),andpHandcalciumconcentrations(BayerDiag...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第8期Agonist-DrivenConformationalChangesintheInner-Sheetof7NicotinicReceptors
...heT7RNApolymeraseandmMessagemMachinekitasdescribedbythemanufacturer(Ambion,Austin,TX).Oocyteswereinjectedwith20ngofcRNAandincubatedat18°CinND96(96mMNaCl,2mMKCl,1mMMgCl2,1.8mMCaCl2,and5mMHEPES,pH7.5)for2to5daysbeforeuse.DataCollectionandAnalysis.Oocytesweresuperfusedinnormalextracellularsolutionc...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第5期