ProteasomalDegradationofHumanCYP1B1:EffectoftheAsn453SerPolymorphismonthePost-TranslationalRegulationofCYP1B1Expression
...athadbeenpreviouslypreabsorbedwithrabbitserum.CellularPhosphorylationAssay.32Pcelllabelingwasperformedasdescribedpreviously(MeekandMilne,2000)withminormodifications.Inbrief,COS-1cellsexpressingeitherCYP1B11orCYP1B14wereincubatedfor3hwithphosphate-freeDMEM(Sigma-Aldrich)supplementedwith5%dialyzedFBS,...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第2期第五節 卵巢腫瘤
...預防腫瘤復發可能起到較好的效果。亦可用放射性同位素32P腹腔內注入。 4.免疾治療 是近年來興起的一種輔助的治療手段,其目的爲增強機體免疫功能,特異性殺傷腫瘤細胞,目前已進入臨牀使用階段。 手術、化療...
醫源資料庫;醫源圖書館;教材類;婦產科學MultipleCopiesofthe16SrRNAGeneinNocardianovaIsolatesandImplicationsforSequence-BasedIdentificationProcedures
...iouslydescribed(7,9,10).DNAobtainedfromthetypestrainofN.novawaslabeledwith[32P]dCTPbyusinganicktranslationkit(InvitrogenCorporation).ThehybridizationmethodfordeterminationofDNArelatednessbyabsorbancetohydroxyapatitehasbeendescribedpreviously(3).Allreactionswereperformedinduplicateat70°C.Therelative...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第6期北京大學醫學部生物化學試題(碩士)2004年-2000年,1996年-1992年
...酸E、乙酰乙酸⑽Hersbeg和chase以T20噬菌體爲實驗材料,以32P標記DNA同時用35S標記蛋白質中的氨基酸,經幾世傳代後,32S放射活性幾乎同時,32P放射活性卻保持相當時間,這實驗有力地證明A、T2噬菌體內的32P活性強B、T2噬菌體內蛋...
醫源資料庫;醫源習題集;生物化學LiverXreceptorsregulateadrenalcholesterolbalance
...lydifferentrelativetoTK-Luc.(C)Electromobilityshiftassayswereperformedwith[32P]-labeledLXRE(–200)andinvitro–transcribedFLAG-hLXRand/orhumanRXR(hRXR).Competitionswereperformedusingunlabelednucleotidesat100-fold,200-fold,or500-foldmolarexcess,asindicated.Forantibody-bindingreactions,25μgofpreimmu...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第7期NF-BregulationofendothelialcellfunctionduringLPS-inducedtoxemiaandcancer
...visualizedeitherbyUVfluorescence(upper2panels)orhybridizationwithIBorGAPDH[32P]dCTP-radiolabeledprobes(lower2panels).OnesetofBMwasharvestedfrommice7daysafterLPSstimulation(2μg/g).(C)IBandsIBproteinexpressionwasevaluatedbyasensitiveimmunoblotting(anti-FLAGM5mAb;Sigma-Aldrich)/immunoprecipitation(IBp...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第11期CardiacAnkyrinRepeatProtein(CARP)ExpressioninHumanandMurineAtheroscleroticLesions
...-containingbuffersaccordingtostandardprocedures.Probeswereradiolabeledwith[32P]-ATP(Amersham)applyingtherandomprimerlabelingmix(GIBCOBRL).TodetecthumanCARPmRNA,acDNAprobeof807bp(454-to1261-bpfragmentofGenBankNo.)wasappliedandforGAPDHafragmentof65bp(480to545bpofGenBankNo.).SM-actinandSM22mRNAweredete...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2003年第23卷第1期1-3分子生物學發展簡史
...肺炎球菌轉化因子是DNA;1952年A.D.Hershey和M.Cha-se用DNA35S和32P分別標記T2噬菌體的蛋白質和核酸,感染大腸桿菌的實驗進一步證明了是遺傳物質。在對DNA結構的研究上,1949-52年S.Furbery等的X-線衍射分析闡明瞭核苷酸並非平面的空間...
醫源資料庫;醫源圖書館;教材類;分子生物學Theflavonoidsquercetinandcatechinsynergisticallyinhibitplateletfunctionbyantagonizingtheintracellularproductionofhydrogenperoxide
...hplateletfunctionbyvirtueofanantioxidanteffect.MATERIALSANDMETHODSMaterials32Piand[3H]oleicacidwerefromAmersham(ArlingtonHeights,IL).Fura2-AMand2‘,7‘-dichlorofluoresceindiacetate(DCFH-DA)werefromMolecularProbes(Eugene,OR)andSepharose2BwasfromPharmacia(Uppsala,Sweden).TetrapeptideArg-Gly-Asp-Ser(...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年72卷第5期MolecularAnalysisoftheInteractionofBordetellapertussisAdenylylCyclasewithFluorescentNucleotides
...edcalmodulinfrombovinebrainwaspurchasedfromCalbiochem(Darmstadt,Germany).[-32P]ATP(800Ci/mmol)waspurchasedfromPerkinElmerLifeandAnalyticalSciences,Waltham,MA.Aluminumoxide90active,neutral(activity1;particlesize,0.06–0.2mm)waspurchasedfromMerck(Darmstadt,Germany).Albuminfrombovineserum,fraction...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第9期