PhosphorylationofGsInfluencesItsAssociationwiththeμ-OpioidReceptorandIsModulatedbyLong-TermMorphineExposure
...xpelledandwashedextensivelyinKrebsbuffer(4°C),andmembraneswereprepared.32PiLabelingofMOR-CHOCellsOnthedayofharvest,cellswereincubatedfor2hinphosphate-andserum-freeDMEMat37°Cundernormalcultureconditions.Later,MOR-CHOcellswerewashedoncewith10mlofphosphate-andserum-freemediaandincubatedwith10ml...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第9期MSH2–MSH6stimulatesDNApolymerase,suggestingaroleforA:Tmutationsinantibodygenes
...T:G,X=T;andforU:G,X=U.Thebottomstrandoligonucleotidewas5‘endlabeledusing[32P]-ATPandT4polynucleotidekinase,followedbypurificationthroughaProbeQuantcolumn(AmershamBiosciences).ThelabeledstrandwasthenannealedtoanequimolaramountoftopstrandcontainingC,T,orU.TogeneratetheabasicanddRPsubstrates,theduple...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第4期分析人工髖關節置換術後骨溶解及假體鬆動的分子生物學機制
...人工髖關節置換術後產生的骨溶解及假體鬆動具有預防及治療作用。【參考文獻】1ArchibeckMJ,JacobsJJ,RoebuckKA,etal.Thebasicscienceofperiprostheticosteolysis.InstrCourseLect,2001,50:185-195.2MirraJM,MarderRA,AmstutzHC.Thepathologyoffailedtotaljointarthroplasty.ClinOrth...
醫源資料庫;在線期刊;中華醫學研究雜誌;2007年第7卷第9期MolecularmechanismsofenhancedrenalcelldivisioninprotectionagainstS-1,2-dichlorovinyl-L-cysteine-inducedacuterenalfailureanddeath
...eotideswerepurifiedbypolyacrylamidegelelectrophoresisandthenendlabeledwith[32P]ATPbyusingT4polynucleotidekinase(68).Aliquotsofnuclearprotein(20μg)wereincubatedwith0.2ngof[32P]endlabeleddouble-strandoligonucleotideprobe.Themixturewasincubatedfor30minatroomtemperatureandthenseparatedbyelectrophoresis...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第7期TheBRAF–MAPKsignalingpathwayisessentialforcancer-immuneevasioninhumanmelanomacells
...lysis.EMSA.5μgofnuclearextractswasmixedwith5xbindingbuffer,polyd(I-C),and[32P]ATP-labeledSTAT3double-strandedoligonucleotideprobewithorwithouteitherunlabeledwild(5‘-GATCCTTCTGGGAATTCCTAGATC-3‘)ormutant(5‘-GATCCTTCTGGGCCGTCCTAGATC-3‘)STAT3double-strandedoligonucleotideprobe.Themixedsampleswe...
醫源資料庫;在線期刊;實驗醫學雜誌;2006年第203卷第7期Reciprocalregulationofairwayrejectionbytheinduciblegas-formingenzymeshemeoxygenaseandnitricoxidesynthase
...SpecificmRNAswereidentifiedbyhybridizationtotheindicatedprobeslabeledwith-[32P]dCTP.Normalizedabsorptionvalueswereobtainedbydensitometryscanningoflabeledbands(NationalInstitutesHealthImage1.62;NationalInstitutesofHealth),including-actinbands.Expressionofeachbandwasnormalizedwithitscorresponding-acti...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第202卷第2期SubstrateinteractionsinthehumantypeIIasodium-phosphatecotransporter(NaPi-IIa)
...oltage-clampandisotopeflux.First,bymeasuringsimultaneousuptakeofasubstrate(32Pi,22Na)andchargeinvoltage-clampedoocytes,weestablishedthatthehumanNaPi-IIaisoformoperateswithaNa:Pi:chargestoichiometryof3:1:1andthatthepreferredtransportedPispeciesisHPO42.Wethenprobedthecomplexinterrelationshipofsubstrat...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第5期枯草桿菌對氨茴環黴素的抗性
...度離心純化、瓊脂糖凝膠電泳、Southernblotting、bmr片段的32P標記和雜交。 5.DNA製備和基因克隆:用標準方法[10]從突變株DM104中提取染色體DNA。用Sau3AI酶部分降解DNA。質粒pCB20用BamHⅠ酶切開,用小牛腸道鹼性磷酸酶去磷酸化...
合作平臺;醫學論文;臨牀醫學與專科論文;檢驗醫學AmutationintheIcsbp1genecausessusceptibilitytoinfectionandachronicmyeloidleukemia–likesyndromeinBXH-2mice
...m.SequencelengthpolymorphismsweregenotypedbyastandardPCR-basedmethodusing-[32P]dATPlabelingandseparationondenaturing9%polyacrylamidegels(42).SNPsfromtheMylsregionandinformativeforA/JandC57BL/6J(BXH-2)wereobtainedfromtheCeleraDiscoverySystemandCelera‘sassociateddatabases(mCV25191372,mCV22708293,and...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第6期AngiotensinIIdirectlystimulatesactivityandaltersthephosphorylationofNa-K-ATPaseinratproximaltubulewitharapidtimecourse
...m.Underthesesameconditions,exposingproximaltubulestoANGIIalteredtheamountof32Pincorporatedintomultiplephosphopeptidesgeneratedfromatrypticdigestofthe-subunitofNa-K-ATPase.Na-K-ATPasewasisolatedfromwholecelllysatesbymeansofaouabain-affinitycolumnandthenseparatedintoitsindividualsubunitsbySDS-PAGE.Na-...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第10期