MSH2–MSH6stimulatesDNApolymerase,suggestingaroleforA:Tmutationsinantibodygenes
...T:G,X=T;andforU:G,X=U.Thebottomstrandoligonucleotidewas5‘endlabeledusing[32P]-ATPandT4polynucleotidekinase,followedbypurificationthroughaProbeQuantcolumn(AmershamBiosciences).ThelabeledstrandwasthenannealedtoanequimolaramountoftopstrandcontainingC,T,orU.TogeneratetheabasicanddRPsubstrates,theduple...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第4期第二節 cRNA探針在原位雜交組織化學
...ml,孵育15~20min。消化時間應根據組織的種類,厚度反覆試驗調整。時間過短探針不易進入,過長影響細胞或組織的形態結構。 (4)0.1mol/L甘氨酸/PBs5min終止蛋白激酶K反應。0.25%乙酸酐(0.1mol/l三乙醇胺配製)10min。 (5...
醫源資料庫;醫源圖書館;教材類;實用免疫細胞與核酸非放射性標記物BrdU研究進展
...死亡細胞干擾 三、BrdU標記和3H-TdR標記的比較 與32P、35S等常用放射性核素相比,3H-TdR釋放的β-粒子能量低,散射極少,因此感光乳膠上的成影分辨最高,放射性損傷相對較小,且半衰期較長。3H-TdR標記用放射自顯影術和...
合作平臺;醫學論文;臨牀醫學與專科論文;檢驗醫學MolecularmechanismsofenhancedrenalcelldivisioninprotectionagainstS-1,2-dichlorovinyl-L-cysteine-inducedacuterenalfailureanddeath
...eotideswerepurifiedbypolyacrylamidegelelectrophoresisandthenendlabeledwith[32P]ATPbyusingT4polynucleotidekinase(68).Aliquotsofnuclearprotein(20μg)wereincubatedwith0.2ngof[32P]endlabeleddouble-strandoligonucleotideprobe.Themixturewasincubatedfor30minatroomtemperatureandthenseparatedbyelectrophoresis...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第7期TheBRAF–MAPKsignalingpathwayisessentialforcancer-immuneevasioninhumanmelanomacells
...lysis.EMSA.5μgofnuclearextractswasmixedwith5xbindingbuffer,polyd(I-C),and[32P]ATP-labeledSTAT3double-strandedoligonucleotideprobewithorwithouteitherunlabeledwild(5‘-GATCCTTCTGGGAATTCCTAGATC-3‘)ormutant(5‘-GATCCTTCTGGGCCGTCCTAGATC-3‘)STAT3double-strandedoligonucleotideprobe.Themixedsampleswe...
醫源資料庫;在線期刊;實驗醫學雜誌;2006年第203卷第7期Reciprocalregulationofairwayrejectionbytheinduciblegas-formingenzymeshemeoxygenaseandnitricoxidesynthase
...SpecificmRNAswereidentifiedbyhybridizationtotheindicatedprobeslabeledwith-[32P]dCTP.Normalizedabsorptionvalueswereobtainedbydensitometryscanningoflabeledbands(NationalInstitutesHealthImage1.62;NationalInstitutesofHealth),including-actinbands.Expressionofeachbandwasnormalizedwithitscorresponding-acti...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第202卷第2期SubstrateinteractionsinthehumantypeIIasodium-phosphatecotransporter(NaPi-IIa)
...oltage-clampandisotopeflux.First,bymeasuringsimultaneousuptakeofasubstrate(32Pi,22Na)andchargeinvoltage-clampedoocytes,weestablishedthatthehumanNaPi-IIaisoformoperateswithaNa:Pi:chargestoichiometryof3:1:1andthatthepreferredtransportedPispeciesisHPO42.Wethenprobedthecomplexinterrelationshipofsubstrat...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第5期第二節 放射性碘標記
...記化合物,最好是對可能產生的雜質加以防止。這要在冷試驗中解決。因爲冷試驗所得產品是非放射性的,其化學純度可用常規方法,如溶點、沸點測定,NMR、紅外、紫外譜分析等手段加以鑑定,得到合格產品後,再按同樣方法...
醫源資料庫;醫源圖書館;教材類;實用免疫細胞與核酸AmutationintheIcsbp1genecausessusceptibilitytoinfectionandachronicmyeloidleukemia–likesyndromeinBXH-2mice
...m.SequencelengthpolymorphismsweregenotypedbyastandardPCR-basedmethodusing-[32P]dATPlabelingandseparationondenaturing9%polyacrylamidegels(42).SNPsfromtheMylsregionandinformativeforA/JandC57BL/6J(BXH-2)wereobtainedfromtheCeleraDiscoverySystemandCelera‘sassociateddatabases(mCV25191372,mCV22708293,and...
醫源資料庫;在線期刊;實驗醫學雜誌;2005年第201卷第6期AngiotensinIIdirectlystimulatesactivityandaltersthephosphorylationofNa-K-ATPaseinratproximaltubulewitharapidtimecourse
...m.Underthesesameconditions,exposingproximaltubulestoANGIIalteredtheamountof32Pincorporatedintomultiplephosphopeptidesgeneratedfromatrypticdigestofthe-subunitofNa-K-ATPase.Na-K-ATPasewasisolatedfromwholecelllysatesbymeansofaouabain-affinitycolumnandthenseparatedintoitsindividualsubunitsbySDS-PAGE.Na-...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第10期