Dietarycarbohydratemodificationinducesalterationsingeneexpressioninabdominalsubcutaneousadiposetissueinpersonswiththemetabolicsyndrome:the
...pleswerecollectedaftera12-hfast.PartofthesampleswasstoredinRNAlater(Ambion,Austin,TX)at4°CforlaterRNAextraction.After24h,RNAlaterwasremovedandthesampleswerestoredat80°CuntilRNAextraction.Adipocytecellsizewasdeterminedfromthesamesamplesasfollows.Afterbeingwashed,theadipocyteswereisola...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2007年85卷第5期CardiacConductionthroughEngineeredTissue
...00HzwithaDAQCard6036E16-bitPCMCIAdataacquisitiondevice(NationalInstruments,Austin,TX)andcollectedusingLabVIEW6software(NationalInstruments).Perfusedheartswereloadedwith5µmol/Ldi-8-ANEPPSfor5minutes(MolecularProbes),andopticalmappingwasperformedusingalaser-scanningsystem.22,23Toeliminatecardiac...
醫源資料庫;在線期刊;美國病理學雜誌;2006年第168卷第7期Increasedbloodpressureintransgenicmiceexpressingbothhumanreninandangiotensinogenintherenalproximaltubule
...r,Cincinnati,OH).RNaseprotectionassayswereperformedusinganRPAIIIkit(Ambion,Austin,TX).Inbrief,30µgofRNAwerehybridizedtohRENand-actinor28Sprobeslabeledwith[-32P]UTPbyinvitrotranscriptionandpurifiedthroughaSephadexG-50spincolumn(RocheMolecularBiochemicals,Indianapolis,IN).ProtectedfragmentsforhR...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第5期Peritubularcapillarydysfunctionandrenaltubularepithelialcellstressfollowinglipopolysaccharideadministrationinmice
...RGreenSupermixfromBio-Rad(Hercules,CA);DNaseTreatment&RemovalKitfromAmbion(Austin,TX);antibodiesusedinWesternblotandimmunohistochemistryfromUpstateCellSignalingSolutions(LakePlacid,NY);SupersignalWestPicochemiluminescencedetectionkitfromPierceBiotechnology(Rockford,IL);andDakoCytomationLSAB+System-H...
醫源資料庫;在線期刊;美國生理學雜誌;2007年第290卷第1期CharacterizationofammoniatransportbythekidneyRhglycoproteinsRhBGandRhCG
...withSP6RNApolymerasefromthelinearizedplasmidDNAwithmMessagemMachine(Ambion,Austin,TX).Oocyteinjection.StageVandVIdefolliculatedoocytesobtainedasdescribed(16)wereinjectedwith2334nl(1μg/μl)ofcRNA,orwaterforcontrol,andplacedinindividualwellsin96-wellplatescontaining200μlSOScontaining(inmM)100NaCl,2K...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第3期RoleofHumanNucleosideTransportersintheCellularUptakeofTwoInhibitorsofIMPDehydrogenase,TiazofurinandBenzamideRiboside
...sinthepresenceofthem7GpppGcap,usingtheMEGAscripttranscriptionsystem(Ambion,Austin,TX).RemainingtemplatewasremovedbyDNAase1digestion.Oocyteswerethenmicroinjectedwitheither20nlofwateralone(controls)or20nlofwatercontaining20ngofRNAtranscripts(testsamples).Unlessotherwiseindicated,uptakeof[3H]BRand[3H]T...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第1期InfantsdiscriminatebetweennaturalandsyntheticvitaminE
...opSoftware(Perkin-Elmer)installedinaDellOptiPlex133MHZPentiumcomputer(Dell,Austin,TX).RedbloodcelltocopherolsRBCtocopherolswereextractedandanalyzedbytheHPLCmethodreportedbyMinoetal(28)withtheuseofascorbateandpyrogallolaspreservatives.Tocolwasusedasaninternalstandard.Thetocopherolswereelutedonarevers...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2003年77卷第4期NegativeRegulationofTRPC3ChannelsbyProteinKinaseC-MediatedPhosphorylationofSerine712
...:1eC12.PutneyJWJr(1997)CapacitativeCalciumEntry.LandesBiomedicalPublishing,Austin,TX.SinghBB,LockwichTP,BandyopadhyayBC,LiuX,BollimunthaS,BrazerSC,CombsC,DasS,LeendersAG,ShengZH,etal.(2004)VAMP2-dependentexocytosisregulatesplasmamembraneinsertionofTRPC3channelsandcontributestoagonist-stimulatedCa2+i...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第2期Temporalpatternofdenovolipogenesisinthepostprandialstateinhealthymen
...whichwereanalyzedwithanHP5973MSfittedwithanETPelectronmultiplier(SGEIncorp,Austin,TX).Comparableionpeakareasbetweenanunlabeledstandardandbiologicalsampleswereachievedbyeitheradjustingthevolumeinjected,diluting,orconcentratingthesamplewhenneeded.NewlymadefattyacidsfromDNLwerecalculatedbymassisotopome...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2005年81卷第1期Mutationsintheextracellularloopof-rENaCaltersensitivitytoamilorideandreactivespecies
...asmidDNAusingT7polymeraseaccordingtothemanufacturer‘sinstructions(Ambion,Austin,TX).TheintegrityofthecRNAwasverifiedbydenaturinggelelectrophoresisthrough1%agarose-formaldehydegel(7).Injectionintooocytes.OocyteswereobtainedfromappropriatelyanesthetizedadultfemaleX.laevistoads(XenopusExpress,BurleyH...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第6期