EMB基因330密碼子分子DNA探針設計及熒光檢測
...hambutolresistantmycobacteriumtuberculosis(MTB),andtodetectfluorescenceofmutationsiteofembB330codoninliquidbyfluorescencespectrophotometer.MethodsThesoftware,beacondesigner,wasusedtodesignmolecularDNAprobedetectingembB306codonanddetectingfluorescencesignalfromhybridizationbetweentheamplifiedpr...
醫源資料庫;在線期刊;局解手術學雜誌;2009年第18卷第1期CD34、CD59在陣發性睡眠性血紅蛋白尿中的表達及意義
...34表達:PBS洗滌BMMNC1次,細胞沉澱加溶血劑及透膜劑各500μl並輕輕混勻,室溫避光作用10min,PBS洗滌1次,去上清,細胞沉澱用PBS調製成細胞濃度爲106/ml的單細胞懸液。分別取100μl BMMNC懸液加入兩個試管中,對照管中加20μlIgG...
醫源資料庫;在線期刊;濱州醫學院學報;2009年第32卷第5期SurvivinmRNA在腎透明細胞癌組織和外周血中表達的研究
...%的凝膠,經微波爐加熱100s左右,冷卻到微燙手時加入2.5μlEB(0.5μg/μl)充分混勻後灌膠。取RNA溶液3μl,加2μl1×TAE,1μl6×LoadingBuffer,混勻後上樣。在1×TAE的電泳液中,以5V/cm的電壓電泳20min,在紫外燈下觀察RNA...
醫源資料庫;在線期刊;濱州醫學院學報;2008年第31卷第3期脊柱結核病尿脫氧吡啶啉檢測及其臨牀意義
...erculosis,pulmonarytuberculosisandhealthycontrolswere1(4.9±9.8)μmol/molCr.,(6.4±2.6)μmol/molCr,and(6.3±2.0)μmol/molCr.,respectively(Pvalue=0.001,0.000).PatientswithspinaltuberculosishadahigherurinaryexcretionofDPDthanthosewithoutspinaltuberculosis.Therewasno...
醫源資料庫;在線期刊;中國矯形外科雜誌;2008年第16卷第11期多孔明膠微球/磷酸鈣骨水泥的製備及其性能研究
...positeasabonesubstituteinvitroandinvivo.[Methods]GMswerepreparedbyimprovedemulsifiedcold-condensationmethodandmacroporousCPCsweredevelopedusingGMswiththreedifferentweightratios(0%,2.5%and5%).TheporosityandthecompressionstrengthofthethreecompositesweremeasuredtodeterminetheproperweightratioofGMs.Them...
醫源資料庫;在線期刊;中國矯形外科雜誌;2009年第17卷第7期縫隙連接阻斷劑1庚醇對小鼠骨髓基質幹細胞體軟骨分化的影響△
...cdifferentiationwithrecombinanthumanGDF5(100ng/ml)or1heptanol(2.5μmol/L).Theproliferationeffectof1heptanolonMSCswasinvestigatedbyMTTassay.TypeⅡcollagenmRNAandproteinwereexaminedwithRTPCRandimmunocytochemistryrespectivelyandthesulfateglycosaminoglycanwasmeasuredbyAlcianblue.Conn...
醫源資料庫;在線期刊;中國矯形外科雜誌;2007年第15卷第5期健齡寶對免疫力低下小鼠免疫功能的影響
...胞活化;T淋巴細胞亞羣;溶血素類;小鼠EFFECTSOFJIANLINGBAOONIMMUNEFUNCTIONINMICEWITHHYPOIMMUNITYZHAOZHENHUAN,JINGWEILI,SUNXIANGHONG,etal(DepartmentofPharmacy,TheAffiliatedHospitalofQingdaoUniversityMedicalCollege,Qingdao266003,China);[ABSTRACT]ObjectiveToobservetheeffectsofdiffer...
醫源資料庫;在線期刊;齊魯醫學雜誌;2009年第24卷第6期精神分裂症與細胞色素P4502D6基因多態性關聯研究
...6C188TpolymorphismhasnoassociatedwithschizophreniaofChinese,CYP2D6C188Tgenemutationwerenomaincauseofschizophrenia.【Keywords】Cytochrome;P4502D6gene;Polymorphism;Treatment-resistantschizophrenia;Case-controlstudies 細胞色素P4502D6(CytochromeP4502,CYP2D6)是細胞色素類藥物代謝具...
醫源資料庫;在線期刊;中國民康醫學;2007年第19卷第5期福辛普利防治兔骨關節炎的實驗研究
...bitarthritiswithfosinopril. LIJie-yi,LIUFang,ZUOYu,etal. (YueyangMunicipalSecondPeople’sHospital,Yueyang414000,Hunan,P.R.China) Abstract:ObjectiveToobservetheeffectsoffosinopril,anangiotensin-convertingenzymeinhibitoronthedevelopmentofcartilagestructuralchangesinanexperimental...
醫源資料庫;在線期刊;中國熱帶醫學雜誌;2007年第7卷第12期親和層析法從紅芸豆中純化紅血球凝集素的研究
...sis(SDSPAGE).PHAEcouldpromoteagglutinationofhumanerythrocytesevenat4μg/ml.ConclusionArapidmethodforpurifyingPHAEproductionfromPhaseolusvulgarisbyaffinityresinisestablishedsuccessfully. 【Keywords】erythrohemagglutinin;affinityresin;Phaseolusvulgaris;separationandpurification ...
醫源資料庫;在線期刊;成都醫學院學報;2010年第5卷第2期