MultilocusSequenceTypingofPorphyromonasgingivalisStrainsfromDifferentGeographicOrigins
...310-to420-bpDNAfragmentsoftheeightubiquitouschromosomalgenes,ftsQ,hagB,gdpxJ,pepO,mcmA,recA,pga,andnah,wereamplifiedbyPCRandthensequenced.Thenumberofallelesatindividuallocirangedfrom2to19,andatotalof33allelicprofiles,orsequencetypes(STs),wereidentified.Nucleotidevariationbetweenalleleswaslocatedaton...
醫源資料庫;在線期刊;微生物臨牀雜誌;2006年第44卷第1期ComparisonofReal-TimePCRConventionalPCR,andGalactomannanAntigenDetectionbyEnzyme-LinkedImmunosorbentAssayUsingBronchoalveolarLavageFluidSam
...ithhematologicalmalignancieswhowereundergoingbronchoscopyinourcenterbetweenJanuary2001andJuly2002withinaweekafterthediagnosisoflunginfiltrates(Table1).Thespecimenswerecollectedinsterilevialswithoutconservationmediumandsenttothelaboratorywithin2h.Uponreceipt,eachspecimenwashomogenizedanddividedintoth...
醫源資料庫;在線期刊;微生物臨牀雜誌;2003年第41卷第8期雙J導管在腎移植手術中的應用(附10例報告)
【摘要】目的觀察、瞭解雙J導管在同種異體腎移植手術中的應用效果。方法對10例腎移植患者使用雙J管作支架內引流,並平均隨訪10個月。結果術後雙J管引流可靠,未發現堵管;拔管後未出現輸尿管狹窄、梗阻、輸尿管膀胱吻合...
合作平臺;在線期刊;中華實用醫藥雜誌;2005年第5卷第8期;臨牀醫學大孔吸附樹脂在中草藥研究中的應用進展
...04advancesofApplicationofMacroporousResinInStudyofTraditionalChineseHerbsMIJing-yuSONGChun-qing(ShanghaiUniversityofTraditionalChineseMedicine,Shanghai200032,China)KeyWords:macroporousresin;traditionalChineseherbs;separationandenrichment參考文獻;1中國醫學科學院藥物研究所...
藥品天地;專業藥學;實驗技術;色譜技術;色譜論文EndophthalmitisCausedbyFusariumproliferatum
...isted,andtheeyebegantoshowseverepalpebraledema.Slitlampexaminationshowedconjunctivalhyperemia,hypopyon,capsularfibrosis,andcornealedema,andtheintraocularlenshadmovedtothenasalsite.Intraocularpressurewas28mmHg,andfundusexaminationshowedvitreoushaze.Anaqueoushumorsamplewastakenusinga30-gaugeneedlefoll...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第10期ComparisonofThreeMolecularMethodsUsedforSubtypingofLegionellapneumophilaStrainsIsolatedduringanEpidemicofLegionellosisinRome
...sualorcomputationalmethodsprovedtobedifficultbecausebanddiscriminationissubjective.SBT,proposedbyGaiaetal.forL.pneumophilaserogroup1andnon-serogroup1(12,13),wasalsousedinthisstudy.Wesequencedbothstrandsoftheampliconsoffiveofthesixgenes(flaA,proA,mip,asd,andpilE)suggestedtobeusedforsequencingwiththep...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第10期Predominanceofafr2andralFimbrialGenesRelatedtoThoseEncodingtheK88andCS31AFimbrialAdhesinsinEnteropathogenicEscherichiacoliIsolatesfromR
...inishedbyanextensiontimeof5minat72°C.ForcolonydotblothybridizationforthemajorfimbrialsubunitralGgenes,coloniesofselectedstrainsweregrownovernightonLuria-Bertaniagarplates,transferredtonitrocellulosemembranes,lysedbyalkali,andfixedasdescribedbyMainiletal.(26).TheralG-specificPCRproductwaspurifiedbyu...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第3期MicrobialDNATypingbyAutomatedRepetitive-Sequence-BasedPCR
...dE.colistrains,includingtheprimarycultures.AlthoughtheE.coliisolatesweresubjectedtomultiplecultureconditions,therep-PCRfingerprintsofeachstrainappearedstableandreproducible,asindicatedbytheaveragesimilarity(97.7%)seeninthedendrogramwithinrepeatedsamplesofeachstrain.Theaveragesimilarityofthetwodiffer...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第1期休克低溫致心電圖J波1例
...克。心電圖(見圖1):竇性P波,心率44次/min,各導聯均見J波,除aVR、V1導聯負相外均爲正相,T波(除aVR)均倒置,ST下移0.1~0.2mV,Q—T間期0.62s,並有多個提前出現的寬大畸形QRS波。心電圖診斷:竇性心動過緩,異常J波,頻發室...
資料庫;在線期刊;中華現代內科學雜誌;2004年第1卷第4期UseoftheDiversiLabSystemforSpeciesandStrainDifferentiationofFusariumSpeciesIsolates
...methoddoesnotroutinelydiscriminatebetweenFusariumspecies,eachspecimenwassubjectedtosequencingofbotha480-bpfragmentofthe28SRNAgeneregion(9)anda656-bpfragmentoftheelongationfactor1alpha(EF-1a)generegion(12).Bothsequencingmethodswereusedbecauseithasbeenreportedthatthe28SRNAgeneregionsequencingmaybeinad...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第10期