MembraneorganizationandfunctionofM1andM23isoformsofaquaporin-4inepithelialcells
...ionswereidenticaltothoseusedforthefunctionalmeasurements.Aregionofinterest(ROI)representingthecontourofeachindividualcellmembraneexaminedwasoutlinedutilizingIPLabsoftware,andthemeanpixelintensitywithintheROIwastakenasameasureofunitdensityofAQP4atorneartheplasmamembrane.Thisprocedurewasrepeatedonbatc...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第9期AlternativeTranslationInitiationofHumanRegulatorsofG-ProteinSignaling-2YieldsaSetofFunctionallyDistinctProteins
...swereidentifiedusing488±5nmexcitationandselectedasregionsofinterest(ROIs).RelativeGFPfluorescence(RGSexpression)andfura-2ratiometric(intracellularcalcium)wasdeterminedforeachROIandwascalculatedasmeanpixelfluorescencevalueafter200-and100-msexposure,respectively.Forfura-2imaging,alternatingexci...
醫源資料庫;在線期刊;分子藥理學雜誌;2008年第70卷第1期ExtracellularATP-inducedcalciumsignalinginmIMCD-3cellsrequiresbothP2XandP2Ypurinoceptors
...experiment,eitheranumberofsinglecellsoragroupofcells[i.e.,regionofinterest(ROI)]wasselectedusingthesoftware.Thefluorescentemissionsaspairedsignals,atawavelengthof510nmfromtheROI,weremeasuredaccordinglytoexcitationwavelengthsof340and380nmatatimeintervalofevery3s.Backgroundfluorescencewassubtractedonl...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第8期ModifiedReceptorInternalizationuponCoexpressionof5-HT1BReceptorand5-HT2BReceptors
...latetheinternalizationkineticsofthereceptors,weselected10regionsofinterest(ROIs)ontheplasmamembranepercellandfollowedtherelativeintensitychangesoftheseROIsbytime,includingatleastthreetofiveindividualcellsperexperiment.Datarepresentmorethanfourindependentexperiments.Thekineticcurveswerecorrectedforbl...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第6期NeotendonformationinducedbymanipulationoftheSmad8signallingpathwayinmesenchymalstemcells
...ble.Themeasurementcanbeverifiedbyusingaprogressivelylargerregionofinterest(ROI)andbysubtractingbackgroundsthatarethesame.AnincreaseintheROIhasnoappreciableeffectonthetotalintensitythatismeasured.Beforelightdetection,theratswereanesthetizedwithaketamine-xylazinemixture,whichwasinjectedintraperitoneal...
醫源資料庫;在線期刊;臨牀研究雜誌;2006年第116卷第4期