植物抗蟲基因工程的研究進展
...是從植物中分離出來的抗蟲基因,如蛋白酶抑制劑基因(PI基因)、澱粉酶抑制劑基因、外源凝集素基因等。其中Bt基因和PI基因在農業上利用最廣,本文對這兩類基因的轉基因工程研究作一簡要綜述。 1、Bt毒蛋白基因 1.lBt...
醫藥經濟;生物技術;實驗技術;生物工程下游技術ANaphthoquinoneDerivative,Shikonin,HasInsulin-LikeActionsbyInhibitingBothPhosphataseandTensinHomologDeletedonChromosome10andTyrosinePhosph
...sfarweakerthanthatofpervanadate,awidelyusedtyrosinephosphataseinhibitor,despitetheobservationthattheeffectofshikoninonPKBwasmorepotentthanthatofpervanadate.TheseresultssuggestedthattheinhibitionofPTENprovidesacluetoitspotentinsulin-likeactions.Wealsofoundthatnaphthoquinones,including1,2-naphthoquino...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第9期雌黃(三硫化二砷)誘導HL-60細胞凋亡與壞死的實驗研究
...和增殖抑制的作用。通過對細胞進行AnnexinV和碘化丙錠(PI)雙重染色,應用流式細胞儀可以區分活細胞、凋亡細胞與壞死細胞。結果2.5μmol/LAs2S3作用18h後誘導HL-60小部分細胞凋亡和大部分細胞壞死,7.5μmol/LAs2S3作用8h後可引起HL-60...
醫源資料庫;在線期刊;中華中西醫雜誌;2006年第7卷第6期PredictorsofHIVDrug-ResistanceMutationsinaLargeAntiretroviral-NaiveCohortInitiatingTripleAntiretroviralTherapy
...ishColumbia,Canada.Methods.Allplasmasampleswithplasmavirusloads(pVLs)1000copies/mLcollectedduringthefirst30monthsoffollow-upweregenotypedfordrugresistance.Theprimaryoutcomemeasurewastimetothefirstdetectionofmajordrug-resistancemutation(s).Coxproportionalhazardregressionwasusedtoidentifyfactorssignif...
醫源資料庫;在線期刊;傳染病學雜誌;2005年第191卷第3期RegulationofHumanConeCyclicNucleotide-GatedChannelsbyEndogenousPhospholipidsandExogenouslyAppliedPhosphatidylinositol3,4,5-trisphosphate
...CNGA3+CNGB3channelswerepreventedbyinhibitionofphosphatidylinositol3-kinase(PI3-kinase)usingwortmanninor2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-onehydrochloride(LY294002),whichsuggeststhatphospholipidmetabolismcanregulatethechannels.Directapplicationofphosphatidylinositol3,4,5-trisphosphate(PIP...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第1期TCD及轉頸試驗在椎動脈型頸椎病中的應用價值
...暈,並與體位變換、頭部過度伸曲或過度旋轉有關,可伴頭痛、視物不清、耳鳴、噁心、嘔吐,少數患者有肢體突然麻木、感覺異常等。頸椎片結果:頸椎生理曲度變直68例,不同程度的頸椎體增生226例,椎間隙狹窄80例,椎間盤...
合作平臺;在線期刊;中華醫學實踐雜誌;2004年第3卷第8期;醫學影像SpondylitisDuetoMycobacteriumxenopiinaHumanImmunodeficiencyVirusType1-InfectedPatient:CaseReportandReviewoftheLiterature
ServicedeMaladiesInfectieusesetTropicalesAServiced‘AnatomopathologieServicedeRhumatologieServicedeBacteriologie,HpitalBichat-ClaudeBernard,Paris,FranceABSTRACTNontuberculousmycobacterialinfectionsarewellknowntooccurinpatientswithhumanimmunodeficiencyvirusinfection.However,spondylitisduetomycobacte...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第3期ASingleAminoAcidAlterationinCytoplasmicDomainDeterminesIL-2PromoterActivationbyLigationofCD28butNotInducibleCostimulator(ICOS)
...theCD28cytoplasmicdomainisaknownbindingsiteforphosphatidylinositol3-kinase(PI3-K)andGrb2.ICOSpossessestheYMFMmotifinthecorrespondingregionofCD28thatbindsPI3-KbutnotGrb2.WepostulatedthatthereasonthatICOSdoesnothavetheabilitytoinduceIL-2productionisbecauseitfailstorecruitGrb2.Toverifythishypothesis,we...
醫源資料庫;在線期刊;美國病理學雜誌;2003年第162卷第2期ASingleAminoAcidAlterationinCytoplasmicDomainDeterminesIL-2PromoterActivationbyLigationofCD28butNotInducibleCostimulator(ICOS)
...theCD28cytoplasmicdomainisaknownbindingsiteforphosphatidylinositol3-kinase(PI3-K)andGrb2.ICOSpossessestheYMFMmotifinthecorrespondingregionofCD28thatbindsPI3-KbutnotGrb2.WepostulatedthatthereasonthatICOSdoesnothavetheabilitytoinduceIL-2productionisbecauseitfailstorecruitGrb2.Toverifythishypothesis,we...
醫源資料庫;在線期刊;實驗醫學雜誌;2003年第197卷第2期PhospholipaseA2-ModifiedLow-DensityLipoproteinActivatesthePhosphatidylinositol3-Kinase-AktPathwayandIncreasesCellSurvivalinMonocyticCells
...ivestress-inducedcelldeath,aneffectabolishedbyphosphatidylinositol3-kinase(PI3K)inhibition.Inaddition,PLA-LDLincreasedsurvivalofhumanmonocytes.WenoticedthatlipidproductsderivedfromLDLphospholipolysisaremediatorsofPLA-LDL-inducedAktactivation.Arachidonicacid,whichisreleasedonphospholipasetreatmentofL...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2006年第26卷第11期