IsolationandcharacterizationoftheXenopusoocyteplasmamembrane:anewmethodforstudyingactivityofwaterandsolutetransporters
...ATCC(Manassas,VA).CappedcRNAwaspreparedwithanmMessagemMachinekitfromAmbion(Austin,TX).Oocyteswereinjectedwith10ngRNAandincubatedat18°Cfor3days.Expressionwasconfirmedbyplacingoocytesindistilledwaterandmonitoringtheswellingandburstingtimesbeforemembranepreparation.ExpressionofmouseUT-A3.MouseUT-A3(Ge...
醫源資料庫;在線期刊;美國生理學雜誌;2005年第288卷第7期Effectsofmaternaldocosahexaenoicacidintakeonvisualfunctionandneurodevelopmentinbreastfedterminfants
...150;32.SlossonR,NicholsonC,HibpshmanT.SlossonIntelligenceTestrevised.Austin,TX:Pro-Ed,Inc,1998.MoosRH.FamilyEnvironmentScaleformRitembooklet.RedwoodCity,CA:MindGarden,2002.TellerD.TellerAcuityCardhandbook.Dayton,OH:VistechConsultants,1989.MayerDL,DobsonV.Gratingacuitycards:validityandreliabili...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2005年82卷第1期TheGln4863AlaMutationwithinaPutative,Pore-LiningTrans-MembraneHelixoftheCardiacRyanodineReceptorChannelAltersBoththeKineticsofRyanoidInte
...rsat1and5kHzandthensampledat20kHzwithaPCI-6036eADboard(NationalInstruments,Austin,TX)foracquisitionandviewingusingAcquire4.0/5.0.1(BruxtonCorporation,Seattle,WA).DatawereexportedfromReview4.0(Bruxton)asASCIItextfilesandwerereformattedwithActivePerl5.8(ActiveState,Vancouver,BC,Canada).Single-channelc...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第3期TheSizeofaSingleResidueoftheSulfonylureaReceptorDictatestheEffectivenessofKATPChannelOpeners
...ncing,linearized,andtranscribedinvitrousingtheT7mMessagemMachinekit(Ambion,Austin,TX).cRNAswereelectrophoresedonformaldehydegels,andconcentrationswereestimatedfromtwodilutionsusingRNAmarkerasastandard.cRNAscodingKir6.2(2ng)andwild-typeormutantSURs(6ng)werecoinjectedintodefoliculatedX.laevisoocytes.I...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第4期AngiotensinII,interstitialinflammation,andthepathogenesisofsalt-sensitivehypertension
...formedinconscious,restrainedratsbytail-cuffplethysmography(NarcoBiosystems,Austin,TX).Ratswereconditionedtwicebeforethebloodpressurewasmeasuredatabasalperiod,everyweekduringthefirst2wkandevery2wkfortherestofthestudy.MicropunctureExperimentsFormicropuncturestudies,theratswereanesthetizedwithpentobarb...
醫源資料庫;在線期刊;美國生理學雜誌;2006年第289卷第12期SpontaneousCoronaryVasospasminKATPMutantMiceArisesFromaSmoothMuscleeCExtrinsicProcess
...ndedindiethylpyrocarbonate-treatedwaterandDNasetreated(TurboDNAfree,Ambion,Austin,Tex).ComplementaryDNAwassynthesizedusingSuperscriptIIIreversetranscriptase(Invitrogen).Transgene-specificprimerswereusedtodetectproductsfromtheSM22-SUR2Btransgene(SM22promoterforward:5‘AGACACCGAAGCTACTCTCC3‘,SUR2ex...
醫源資料庫;在線期刊;循環研究雜誌;2006年第96卷第3期OsteogenicResponsesinFibroblastsActivatedbyElastinDegradationProductsandTransformingGrowthFactor-ß
....OnemicrogramoftotalRNAwasthenreversetranscribedusingRetroScriptkit(Ambion,Austin,TX).ThecDNAsamplewasfurtheramplifiedonaRotorgene3000thermalcycler(CorbettResearch,Mortlake,NSW,Australia)andusingQuantiTectSYBRGreenPCRkit(Qiagen),whichallowsforreal-timequantitydetectionofpolymerasechainreaction(PCR)p...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第7期MolecularPharmacologyofAdenosineTransportinTrypanosomabrucei:P1/P2Revisited
...,followedbyethanolprecipitation.AfterDNasetreatment(DNA-away;AmbionBiotech,Austin,TX),cDNAwassynthesizedfrom1eofRNAwithavianmyeloblastosisvirusreversetranscriptase(Roche,Mannheim,Germany)andT16primerinavolumeof15e.PCRwasperformedwithTaqpolymerase(QIAGEN,Hilden,Germany)on3eofcDNA.Negativecontrolslack...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第3期MicroRNAsAreAberrantlyExpressedinHypertrophicHeart
...NAswereisolatedfrommouseheartsusingthemirVanamiRNAisolationkit(Ambion,Inc.,Austin,TX).miRNAexpressionprofilingwasdeterminedbymiRNAmicroarrayanalysisusingthemousemiRNAarrayprobes(ChipIDmiMouse7.1version;LCScience,Houston,TX)thatinclude233maturemousemiRNAs.QuantitativeReverseTranscriptase-PolymeraseCh...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第6期-IsExpressedinHepaticMyofibroblastsandNotOvalCellsinStemCell-MediatedLiverRegeneration
...lysisbuffer.TotalRNAwasisolatedbyRNAqueousmicrokit(catalogno.AM1931;Ambion,Austin,TX).AhighcapacitycDNAreversetranscriptionkit(catalogno.4368814;ABI)wasusedforcDNAsynthesisasrecommendedbythesupplier.PCRwasperformedbytheABIPrism7300sequencedetectionsystem(AppliedBiosystems,Weiterstadt,Germany),usingA...
醫源資料庫;在線期刊;美國病理學雜誌;2007年第169卷第11期