GrowthofPulmonaryMicrovasculatureinVentilatedPretermInfants
...ghed.BiopsiestakenfromtherightupperlobeweretreatedwithRNAlater(Ambion,Inc.,Austin,TX),asdescribedpreviously(21).Theremainderoftherightlungwasimmersedinformalin.Theleftlungwasinflation-fixedwithformalinatastandardizedpressureof20cmH2O.Afterovernightfixation,thevolumeoftheleftlungwasmeasuredbyvolumedi...
醫源資料庫;在線期刊;美國呼吸和危急護理醫學;2006年第173卷第2期DetectionofThymidylateSynthaseModulatorsbyaNovelScreeningAssay
...asdetectedasdescribedabove.RT-PCR.RNAwaspreparedusinganRNAqueouskit(Ambion,Austin,TX).ReversetranscriptionwasdoneusingaCells-to-cDNAkit(Ambion).Polymerasechainreaction(PCR)wasperformedasdescribedpreviously(Dolnicketal.,1992).PrimersusedforTSmRNA[5‘-TTTGGACAGCCTGGGATTCTC-3‘(sense)and5‘-AAAGCACC...
醫源資料庫;在線期刊;分子藥理學雜誌;2003年第63卷第1期Preventionofnerveconductiondeficitindiabeticratsbypolyunsaturatedfattyacids
...cquisitionboard(AT-MIO-16)andtheprogramLABVIEW(bothfromNationalInstruments,Austin,TX).Actionpotentialsfrombothsiteswereaveraged68times.Conductionvelocitywascalculatedfromthedifferenceinlatenciesoftheactionpotentialsandthenervelengthseparatingthe2cathodestimulationpoints.NervedissectionRatswere...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年71卷第1期EvaluationoftheMagNAPureLCInstrumentforExtractionofHepatitisCVirusRNAfortheCOBASAMPLICORHepatitisCVirusTest,Version2.0
...cience)fortechnicalassistance.WethankDwightB.DuBois(CenetronDiagnosticsLLC,Austin,Tex.)forprovidingWHOstandard96/790.Wealsothankallofthemembersofourhepatitis/HIVlaboratorywhomadethisstudypossible.ThisstudywasreviewedandapprovedbytheMayoInstitutionalReviewBoard(no.2002-01). REFERENCESTopAbstractInt...
醫源資料庫;在線期刊;微生物臨牀雜誌;2003年第41卷第8期MicrobiologicalAspectsoftheInvestigationThatTracedthe1998OutbreakofListeriosisintheUnitedStatestoContaminatedHotDogsandEstablishmentofM
...gricultureandFoodSafetyandInspectionService,Washington,D.C.Dalton,C.B.,C.C.Austin,J.Sobel,P.S.Hayes,W.F.Bibb,L.M.Graves,B.Swaminathan,M.E.Proctor,andP.M.Griffin.1997.AnoutbreakofgastroenteritisandfeverduetoListeriamonocytogenesinmilk.N.Engl.J.Med.336:100-105.Evans,M.R.,B.Swaminathan,L.M.Graves,E.Alt...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第5期Niche-DependentTranslineageCommitmentofEndothelialProgenitorCells,NotCellFusioninGeneral,IntoMyocardialLineageCells
...y7daysaftercoculture.RT-PCRCoculturedcellswerelysedinRNAlysisbuffer(Ambion,Austin,Tex).However,EPCorPBS-injectedmyocardialsampleswerehomogenizedinRNAlysisbuffer.RNAwasextractedusingRNAextractionkit(Ambion).DNAasedigestionwasperformedafterRNAextraction.TheRT-PCRwasperformedbyasystemaccordingtothemanu...
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2005年第25卷第7期DevelopmentofOne-Step,Real-Time,QuantitativeReverseTranscriptasePCRAssaysforAbsoluteQuantitationofHumanCoronavirusesOC43and229E
...gofthePCRproductwasusedforinvitrotranscription(MEGAshortscriptT7kit;Ambion,Austin,TX),performedat37°Covernightina20-μlreactionmixturecontaining2μlofreactionbuffer,2μlofeachnucleosidetriphosphate,and2μlenzymemix.ThecDNAswereremovedbydigestionwith2UofRNase-freeDNaseIfor15minat37°C.ThecRNAswerepr...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第11期MolecularEpidemiologyofCommunity-andHealthCare-AssociatedMethicillin-ResistantStaphylococcusaureusinManitoba,Canada
...em(Mandel,Guelph,Canada)andanalyzedusingBionumericsversion3.0(AppliedMaths,Austin,TX).IsolatesrepresentinguniquePFGEpatternsoriginallyidentifiedwithBioImagewerererunforinclusionintheBionumericsdatabase.DendrogramsreportedinthispaperwereconstructedusingBionumericswiththeDICEcoefficientandtheunweighte...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第6期EndothelialCellCortactinPhosphorylationbySrcContributestoPolymorphonuclearLeukocyteTransmigrationInVitro
...ncortactinmRNAandascrambledversionofthissequencewerepurchasedfromAmbionInc(Austin,Tex).BLASTsearcheswereperformedonbotholigonucleotidestoensuretheabsenceofnonspecifictargets.Thesequencesoftheoligonucleotidesare:cortactinsiRNAoligonucleotide(sense),GGGAGAAUGUCUUUCAAGATT;scrambledcontrol,UAAGCUGAUAGUA...
醫源資料庫;在線期刊;循環研究雜誌;2006年第96卷第2期Expressionandrelativeabundanceofshorttransientreceptorpotentialchannelsintheratrenalmicrocirculation
...ntrifugationandresuspendedinTriReagent(Sigma,St.Louis,MO)orRNALater(Ambion,Austin,TX)forextractionofRNAorinLaemmlisamplebuffer(50mMTris·HCl,100mMdithiothreitol,2%SDS,10%glycerol)forSDS-PAGE.Segmentsofthoracicaortaandbrain(cerebrum)wereobtainedfromratsimmediatelyafterremovalofthekidneys.Aortic...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第3期