上海藥物所發現萘酰胺類VEGFR-2酪氨酸激酶抑制劑
...胺類VEGFR-2抑制劑。在這類抑制劑中,代表高活性化合物14c在酶和細胞水平均顯示出很強的抑制活性。激酶譜測試表明,14c是一個多靶點抑制劑,對VEGFR-1,PDGFR-β和RET也有較強的抑制活性。此外,化合物14c在HUVEC管腔形成實驗中,...
醫藥經濟;生物技術;技術要聞PhospholipidTransferProtein–DeficientMiceAbsorbLessCholesterol
...olesteroland[9,10(N)-3H]trioleinwerepurchasedfromNENLifeScienceProducts.[4-14C]Cholesteroland[9,10(N)-3H]oleicacidwerefromAmersham.[5,6-3H]SitostanolwasobtainedfromAmericanRadiolabeledChemicals.MiceandDietAge-andsex-matchedwild-type(WT)andPLTPKOmiceinaC57BL/6backgroundwereinvestigatedinthesestudies....
醫源資料庫;在線期刊;動脈硬化血栓血管生物學雜誌;2007年第27卷第9期現代分子生物學實驗室中放射性同位素輻射的防護和放射性污染的處理
...材料與儀器(1)放射性核素製劑溶液選用三種(32P、3H、14C),分別配成3.7×109Bq/ml。(2)選用稀紗布、2mm厚玻片、2mm厚塑料片、6mm厚塑料片、0.5mm厚鋁鉑片作防護實驗材料,選用塗漆木板、玻璃板、光面瓷磚、毛面瓷磚作爲去...
合作平臺;在線期刊;中華醫學實踐雜誌;2004年第3卷第7期;論著DirectInteractionoftheCellDivisionCycle37HomologInhibitsEndothelialNitricOxideSynthaseActivity
...mineNOreleaseusingacGMPreportercellassayorlysedtodetermineeNOSactivitybyL-[14C]-argininetoL-[14C]-citrullineconversion.ExpressionofMutantS13A-Cdc37Usingsite-directedmutagenesis,wehavepreviouslygeneratedthecDNAforaS13A-Cdc37mutantconstruct.25AnadenoviruscontainingthecDNAforS13A-Cdc37(AdS13A-Cdc37)was...
醫源資料庫;在線期刊;循環研究雜誌;2006年第96卷第2期Sex-DependentExpressionandActivityoftheATP-BindingCassetteTransporterBreastCancerResistanceProtein(BCRP/ABCG2)inLiver
...furantoin,xylazine,andcimetidinewerefromSigma-Aldrich(St.Louis,MO);PhIPand[14C]PhIP(10Ci/mol)werefromTorontoResearchChemicals,Inc.(Toronto,ON,Canada);topotecan(Hycamtin)and[14C]topotecan(56Ci/mol)werefromSmithKlineBeecham(KingofPrussia,PA);[N-methyl-3H]cimetidine(20Ci/mmol)wasfromAmershamBiosciences...
醫源資料庫;在線期刊;分子藥理學雜誌;2005年第67卷第5期Acidosisimpairsinsulinreceptorsubstrate-1-associatedphosphoinositide3-kinasesignalinginmusclecells:consequencesonproteolysis
...cagelthin-layerchromatographyplateswerefromWhatman(Maidstone,Kent,UK);L-[U-14C]phenylalanine(Phe)wasfromNewEnglandNuclear,DuPont(Boston,MA);anti-p85PI3KantibodieswerefromUpstateBiotechnology(LakePlacid,NY);anti-p110PI3KantibodieswerefromSantaCruzBiotechnology(SantaCruz,CA);anti-AKTandphospho-AKTanti...
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第10期NHE3Na+/H+exchangersupportsproximaltubularproteinreabsorptioninvivo
...3-/-)mice.InhibitionofNHE3byEIPAorS-3226reducedthefractionalreabsorptionof[14C]cytochromecby50%duringearlyproximalmicroinfusion.Duringearlydistalmicroinfusion,noproteinreabsorptioncouldbedetected.UrinaryproteinexcretionofNhe3-/-orheterozygousmutantmicewassignificantlyhighercomparedwithwild-typemice....
醫源資料庫;在線期刊;美國生理學雜誌;2004年第287卷第9期Placentaldeliveryofarachidonicanddocosahexaenoicacids:implicationsforthelipidnutritionofpreterminfants
...icprocessisratelimited,especiallybythe6-desaturaseforLAandALA(32).Testswith14C-andtritium-labeledparent,precursor,andproductsfattyacidsshowedthattheproportionsofbrainAArecoveredfromAAitselfwas10timesgreaterthanthatrecoveredfromtheprecursorLA.SimilarresultsweredescribedforacomparisonofALAandDHA(33).T...
醫源資料庫;在線期刊;美國臨牀營養學雜誌;2000年71卷第1期喹諾酮類抗生素那氟沙星
...[4,11,12] 大鼠和犬正常皮膚分別給藥(塗抹)[14C]-那氟沙星7.4mg/kg和2.2mg/kg後的血藥濃度峯值Cmax分別爲42ng/ml和5.8ng/ml,隨後血藥濃度逐漸下降。大鼠損傷皮膚給藥[14C]-那氟沙星7.4mg/kg後的Cmax爲1325ng/ml,Tmax爲1h,t1/2...
醫源資料庫;在線期刊;中華實用醫藥雜誌;2009年第9卷第3期TheRoleofProteinSynthesisandDegradationinthePost-TranscriptionalRegulationofRatMultidrugResistance-AssociatedProtein2(Mrp2,Abcc2)
...atedratsusinginvivometabolic-labelingstudieswith[35S]cysteine/methionineor[14C]NaHCO3,polysomaldistributionanalysesandribonucleaseprotectionassays(RPA).Mrp2proteinwassignificantlyincreasedinratstreatedwithPCNfor2daysbutsignificantlydecreasedin19-daypregnantratsrelativetocontrols;nosignificantdiffere...
醫源資料庫;在線期刊;分子藥理學雜誌;2006年第68卷第3期